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FIGURE: 1 / 1
GPR40 Antibody (PA5-95511) in WB
Western blot analysis of GPR40 in Lane 1: human PANC-1 whole cell lysates. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with GPR40 polyclonal antibody (Product # PA5-95511) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL).
Product Details
PA5-95511
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence of human GPCR GPR40 (YVGCLRALARSGLTHRRKLRAAWVA).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2807313
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human PANC-1 whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
This gene encodes a member of the GP40 family of G protein-coupled receptors that are clustered together on chromosome 19. The encoded protein is a receptor for medium and long chain free fatty acids and may be involved in the metabolic regulation of insulin secretion. Polymorphisms in this gene may be associated with type 2 diabetes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FFAR; Free fatty acid receptor 1; G protein-coupled receptor 40; G protein-coupled receptor GPR40; G-protein coupled receptor 40; HGNC:4498
Gene Aliases: FFA1R; FFAR1; GPCR40; GPR40
UniProt ID: (Human) O14842, (Rat) Q8K3T4, (Mouse) Q76JU9
Entrez Gene ID: (Human) 2864, (Rat) 266607, (Mouse) 233081
G-protein coupled receptor signaling pathway
positive regulation of calcium ion transport into cytosol
insulin secretion
positive regulation of insulin secretion
glucose homeostasis
positive regulation of calcium ion transport
response to fatty acid
signal transduction
positive regulation of GTPase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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