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GPR61 Antibody (PA3-038) in IHC (P)
Immunohistochemistry analysis of GPR61 was performed on human enteric ganglion tissue. To expose target proteins, antigen retrieval was performed by microwaving tissues for 20 minutes in 10mM sodium citrate buffer (pH 6.0). Tissue slides were probed with a GPR61 polyclonal antibody (Product # PA3-038) at a dilution of 1:3000, overnight at 4C in a humidified chamber. Tissues were washed, and detection was pe... View More
Product Details
PA3-038
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated COOH-terminal amino acids of 432-452 (SDIIMSDSYLRPAASPRLES)
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2664682
Product Specific Information
IHC(P) analysis shows positive staining of GPR61 in human pancreatic islets and enteric ganglion.
WB analysis shows GPR61 in glyco-protein enriched fractions from mock transfected or GPR61 overexpressed 293 cells. The GPR61 antiserum showed specific detection of GPR61 over other tested GPRs.
GPR61 exists in different glycosylated forms and can form dimers and oligomers. Consequently it appears as a long smear on WB or is detected at multiple molecular weights.
Target Information
G protein-coupled receptors (GPCRs, or GPRs) contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. GPCR GPR61 is a member of this family (subfamily Orphan-A). It has been reported to be expressed in several regions of brain, including caudate, putamen, and thalamus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Biogenic amine receptor-like G-protein coupled receptor; biogenic amine receptor-like GPCR; G-protein coupled receptor 61
Gene Aliases: BALGR; GPCR3; GPR61
UniProt ID: (Human) Q9BZJ8
Entrez Gene ID: (Human) 83873
Performance Guarantee
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