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GPX1 Antibody (711797) in WB
Western blot analysis was performed on Whole cell extracts (30 µg lysate) of C2C12 (Lane 1), L6 (Lane 2), HT-29 (Lane 3) and tissue extract of Rat Liver (Lane 4). The blots were probed with Anti-GPX1 Recombinant Rabbit Superclonal™ Antibody (Product # 711797, 2.5 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 22 kDa band corresponding to GPX1 was observed across the cell lines and tissue tested. Known quantit... View More
Product Details
711797
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Bovine
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
5HCLC
Immunogen
Peptides corresponding to human GPX1 [1) aa15-aa34 2) aa129-aa144]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2723218
Product Specific Information
This antibody is predicted to react with Monkey, Pig, Bovine.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
Glutathione peroxidases (Gpxs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organic hydroperoxides by glutathione. There are several isoforms which differ in their primary structure and localization. The classical cytosolic/mitochondrial GPx1 (cGPx) is a selenium-dependent enzyme, first of the GPx family to be discovered. GPx2, also known as gastrointestinal GPx (GI-GPx), is an intracellular enzyme expressed only at the epithelium of the gastrointestinal tract. Extracellular plasma GPx (pGPx or GPx3) is mainly expressed by the kidney from where it is released into the blood circulation. Phospholipid hydroperoxide GPx4 (PH-GPx) expressed in most tissues, can reduce many hydroperoxides including hydroperoxides integrated in membranes, hydroperoxy lipids in low density lipoprotein or thymine. All mammalian GPx family members, except for the recently described Cys containing GPx3 and epididymis-specific secretory GPx (eGPx or GPx5) isoforms, possess selenocysteine at the active site.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cellular glutathione peroxidase; Glutathione peroxidase 1; GPx-1; HGNC:4553; MGC14399; MGC88245; OTTHUMP00000210766; Phospholipid-hydroperoxide glutathione peroxidase GPX1; Selenium-dependent glutathione peroxidase 1
Gene Aliases: AI195024; AL033363; CGPx; Gpx; GPx-1; GPX1; GPXD; GSHPx; GSHPx-1; GSHPX1
UniProt ID: (Human) P07203, (Rat) P04041, (Mouse) P11352
Entrez Gene ID: (Human) 2876, (Rat) 24404, (Mouse) 14775
response to reactive oxygen species
temperature homeostasis
endothelial cell development
negative regulation of inflammatory response to antigenic stimulus
lipid metabolic process
triglyceride metabolic process
glutathione metabolic process
apoptotic process
response to oxidative stress
response to lipid hydroperoxide
aging
sensory perception of sound
cell proliferation
intrinsic apoptotic signaling pathway in response to oxidative stress
response to xenobiotic stimulus
response to symbiotic bacterium
response to wounding
response to toxic substance
UV protection
response to hormone
response to glucose
response to selenium ion
response to gamma radiation
response to organic cyclic compound
myotube differentiation
protein oxidation
response to estradiol
response to hydroperoxide
regulation of mammary gland epithelial cell proliferation
response to nicotine
regulation of gene expression, epigenetic
vasodilation
response to hydrogen peroxide
hydrogen peroxide catabolic process
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
skeletal muscle tissue regeneration
regulation of neuron apoptotic process
blood vessel endothelial cell migration
fat cell differentiation
cell redox homeostasis
skeletal muscle fiber development
myoblast proliferation
response to folic acid
interaction with symbiont
positive regulation of protein kinase B signaling
heart contraction
angiogenesis involved in wound healing
regulation of proteasomal protein catabolic process
negative regulation of release of cytochrome c from mitochondria
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
oxidation-reduction process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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