Invitrogen

GPX1 Monoclonal Antibody (8B10)

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Datasheet
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Datasheet
Protocols
Questions & Answers

Product Details

MA5-49180

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL
-

Immunocytochemistry (ICC/IF)

5 µg/mL
-

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

8B10

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human GPX1 (116-146aa EVNGAGAHPLFAFLREALPAPSDDATALMTD).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

0.05mg sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_3074838

Product Specific Information

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Immunogen sequence is different from the related mouse sequence by six amino acids and from the related rat sequence by five amino acids.

Positive Control - WB: human placenta tissue, human U-87 whole cell, human THP-1 whole cell, human U-937 whole cell, human HepG2 whole cell, rat brain tissue, rat thymus tissue, rat lung tissue, rat liver tissue, mouse brain tissue, mouse thymus tissue, mouse lung tissue, mouse liver tissue. IHC: human lung cancer tissue, human mammary cancer tissue. Flow: U87 cell, U251 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Information

Glutathione peroxidases (Gpxs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organic hydroperoxides by glutathione. There are several isoforms which differ in their primary structure and localization. The classical cytosolic/mitochondrial GPx1 (cGPx) is a selenium-dependent enzyme, first of the GPx family to be discovered. GPx2, also known as gastrointestinal GPx (GI-GPx), is an intracellular enzyme expressed only at the epithelium of the gastrointestinal tract. Extracellular plasma GPx (pGPx or GPx3) is mainly expressed by the kidney from where it is released into the blood circulation. Phospholipid hydroperoxide GPx4 (PH-GPx) expressed in most tissues, can reduce many hydroperoxides including hydroperoxides integrated in membranes, hydroperoxy lipids in low density lipoprotein or thymine. All mammalian GPx family members, except for the recently described Cys containing GPx3 and epididymis-specific secretory GPx (eGPx or GPx5) isoforms, possess selenocysteine at the active site.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Cellular glutathione peroxidase; Glutathione peroxidase 1; GPx-1; HGNC:4553; MGC14399; MGC88245; OTTHUMP00000210766; Phospholipid-hydroperoxide glutathione peroxidase GPX1; Selenium-dependent glutathione peroxidase 1

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Gene Aliases: AI195024; AL033363; CGPx; Gpx; GPx-1; GPX1; GPXD; GSHPx; GSHPx-1; GSHPX1

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UniProt ID: (Human) P07203, (Rat) P04041, (Mouse) P11352

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Entrez Gene ID: (Human) 2876, (Rat) 24404, (Mouse) 14775

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Function(s)
glutathione peroxidase activity selenium binding SH3 domain binding glutathione binding phospholipid-hydroperoxide glutathione peroxidase activity peroxidase activity protein binding oxidoreductase activity
Process(es)
response to reactive oxygen species temperature homeostasis endothelial cell development negative regulation of inflammatory response to antigenic stimulus lipid metabolic process triglyceride metabolic process glutathione metabolic process apoptotic process response to oxidative stress response to lipid hydroperoxide aging sensory perception of sound cell proliferation intrinsic apoptotic signaling pathway in response to oxidative stress response to xenobiotic stimulus response to symbiotic bacterium response to wounding response to toxic substance UV protection response to hormone response to glucose response to selenium ion response to gamma radiation response to organic cyclic compound myotube differentiation protein oxidation response to estradiol response to hydroperoxide regulation of mammary gland epithelial cell proliferation response to nicotine regulation of gene expression, epigenetic vasodilation response to hydrogen peroxide hydrogen peroxide catabolic process negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process skeletal muscle tissue regeneration regulation of neuron apoptotic process blood vessel endothelial cell migration fat cell differentiation cell redox homeostasis skeletal muscle fiber development myoblast proliferation response to folic acid interaction with symbiont positive regulation of protein kinase B signaling heart contraction angiogenesis involved in wound healing regulation of proteasomal protein catabolic process negative regulation of release of cytochrome c from mitochondria negative regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway oxidation-reduction process
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