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Invitrogen
GPX3 Polyclonal Antibody
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GPX3 Antibody (PA5-120190) in WB
Western blot analysis of GPX3 in mouse kidney. Samples were incubated with GPX3 Polyclonal antibody (Product # PA5-120190) using a dilution of 1:800, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
PA5-120190
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GPX3 (NP_0020752)
Conjugate
Form
Liquid
Concentration
2.83 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2913762
Product Specific Information
Positive test controls include: HepG2, Mouse kidney, Mouse liver. The target is usually found in the following locations: Secreted.
Immunogen sequence: QSRGQEKSKM DCHGGISG
Target Information
Glutathione peroxidases (Gpxs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organic hydroperoxides by glutathione. There are several isoforms which differ in their primary structure and localization. The classical cytosolic/mitochondrial GPx1 (cGPx) is a selenium-dependent enzyme, first of the GPx family to be discovered. GPx2, also known as gastrointestinal GPx (GI-GPx), is an intracellular enzyme expressed only at the epithelium of the gastrointestinal tract. Extracellular plasma GPx (pGPx or GPx3) is mainly expressed by the kidney from where it is released into the blood circulation. Phospholipid hydroperoxide GPx4 (PH-GPx) expressed in most tissues, can reduce many hydroperoxides including hydroperoxides integrated in membranes, hydroperoxy lipids in low density lipoprotein or thymine. All mammalian GPx family members, except for the recently described Cys containing GPx3 and epididymis-specific secretory GPx (eGPx or GPx5) isoforms, possess selenocysteine at the active site (4-5).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Extracellular glutathione peroxidase; extracellular GPx; Glutathione peroxidase 3; glutathione peroxidase 3 (plasma); GPx-3; GPx-P; Plasma glutathione peroxidase; plasma GPx
Gene Aliases: AA960521; EGPx; GPx; GPx-3; GPx-P; GPX3; GPXP; GSHPx-3; GSHPx-P
UniProt ID: (Human) P22352, (Rat) P23764, (Mouse) P46412
Entrez Gene ID: (Human) 2878, (Rat) 64317, (Mouse) 14778
response to reactive oxygen species
response to lipid hydroperoxide
hydrogen peroxide catabolic process
protein homotetramerization
oxidation-reduction process
cellular oxidant detoxification
response to molecule of fungal origin
glutathione metabolic process
response to oxidative stress
female pregnancy
response to organic substance
response to selenium ion
response to organic cyclic compound
response to drug
response to corticosterone
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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