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Invitrogen
GPX4 Polyclonal Antibody
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GPX4 Antibody (PA5-102521) in ICC/IF
Immunofluorescent analysis of GPX4 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and GPX4 polyclonal antibody (Product # PA5-102521) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-102521
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human GPX4(Accession P36969), corresponding to amino acid residues I170-F197.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2851923
Product Specific Information
Antibody detects endogenous levels of total GPX4.
Target Information
Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, experimental evidence for alternative splicing exists; alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Glutathione peroxidase 4; GPx-4; HGNC:4556; PHGPx; phospholipid hydroperoxidase; Phospholipid hydroperoxide glutathione peroxidase; Phospholipid hydroperoxide glutathione peroxidase GPX4; phospholipid hydroperoxide glutathione peroxidase, mitochondrial; phospholipid hydroperoxide glutathione peroxidase, nuclear; sperm nuclei glutathione peroxidase; sperm nucleus glutathione peroxidase
Gene Aliases: GPx-4; GPX4; GSHPx-4; MCSP; mtPHGPx; PHGPx; SMDS; snGPx; snPHGPx
UniProt ID: (Human) P36969, (Mouse) O70325, (Rat) P36970
Entrez Gene ID: (Human) 2879, (Mouse) 625249, (Rat) 29328
chromatin organization
glutathione metabolic process
response to oxidative stress
multicellular organism development
spermatogenesis
hydrogen peroxide catabolic process
regulation of inflammatory response
oxidation-reduction process
aging
response to estradiol
phospholipid metabolic process
lipoxygenase pathway
cellular oxidant detoxification
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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