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Invitrogen
GRB10 Monoclonal Antibody (5H7)
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GRB10 Antibody (MA5-49206) in WB
Western blot analysis of GRB10 using GRB10 antibody (Product # MA5-49206). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human Hek293 whole cell lysates; Lane 2: human Hela whole cell lysates; Lane 3: monkey COS-7 whole cell lysates; Lane 4: human HepG2 whole cell lysates; Lane 5: rat kidney tissue lysates; Lane 6: rat NRK whole cell lysates. After Electrophoresis, proteins were ... View More
Product Details
MA5-49206
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Non-human primate,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
5H7
Immunogen
E.coli-derived human GRB10 recombinant protein (Position: M1-K251).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3074864
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human Hek293 whole cell, human Hela whole cell, monkey COS-7 whole cell, human HepG2 whole cell, rat kidney tissue, rat NRK whole cell. Flow: THP-1 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
GRB7, a SH2 domain protein, has a single SH2 domain at its C-terminal, a central region with similarity to Ras GAP, and a proline-rich N terminus. A related SH2 domain-containing protein, GRB10, exhibits a high degree of homology with GRB7. GRB10 undergoes serine but not tyrosine phosphorylation in response to EGF treatment, but appears to bind to the EGF receptor poorly. GRB10 maps to mouse chromosome 11, in close proximity to the EGF receptor. Similarly, GRB7 maps to the same mouse chromosome near the EGF receptor-related protein HER2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GRB10 adapter protein; GRB10 adaptor protein; Growth factor receptor-bound protein 10; Insulin receptor-binding protein Grb-IR; maternally expressed gene 1; OTTHUMP00000197988; OTTHUMP00000197989; OTTHUMP00000197990; OTTHUMP00000197991; OTTHUMP00000197993; OTTHUMP00000197994
Gene Aliases: Grb-10; GRB-IR; GRB10; GRBIR; IRBP; KIAA0207; MEG1; RGD1566234; RSS
UniProt ID: (Human) Q13322
Entrez Gene ID: (Human) 2887, (Rat) 498416
negative regulation of Wnt signaling pathway
positive regulation of vascular endothelial growth factor receptor signaling pathway
negative regulation of phosphorylation
negative regulation of glucose import
negative regulation of insulin receptor signaling pathway
insulin-like growth factor receptor signaling pathway
biological_process
insulin receptor signaling pathway
positive regulation of signal transduction
response to insulin
positive regulation of phosphorylation
negative regulation of glycogen biosynthetic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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