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Product Details
MA5-47669
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
M331
Immunogen
Clone (M331) was generated from a full length recombinant rat Grb2 protein. This sequence has high homology to human and mouse Grb2.
Conjugate
Form
Liquid
Concentration
0.125 mg/mL
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2942650
Product Specific Information
This antibody detects a 24 kDa* protein corresponding to the molecular mass of Grb2 on SDS-PAGE immunoblots of rat PC12.
Target Information
GRB 2 protein contains one SH2 domain flanked on both sides by an SH3 domain. In response to EGF, GRB 2 binds, through its SH3 domains, to the guanine nucleotide exchange factor, Sos. The GRB 2-Sos complex then binds the EGF receptor. These interactions play a critical role in the activation of the p21 ras signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: abundant SRC homology; Adapter protein GRB2; ASH protein; epidermal growth factor receptor-binding protein GRB2; Growth factor receptor-bound protein 2; growth factor receptor-bound protein 3; HT027; OTTHUMP00000166097; OTTHUMP00000166098; Protein Ash; SH2/SH3 adapter GRB2
Gene Aliases: AA408164; ASH; EGFRBP-GRB2; GRB2; Grb3-3; MST084; MSTP084; NCKAP2
UniProt ID: (Human) P62993, (Mouse) Q60631, (Rat) P62994
Entrez Gene ID: (Human) 2885, (Mouse) 14784, (Rat) 81504
SH3/SH2 adaptor activity
Ras guanyl-nucleotide exchange factor activity
epidermal growth factor receptor binding
neurotrophin TRKA receptor binding
protein binding
1-phosphatidylinositol-3-kinase activity
SH3 domain binding
protein kinase binding
protein phosphatase binding
identical protein binding
insulin receptor substrate binding
poly(A) RNA binding
ephrin receptor binding
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
phosphotyrosine binding
protein domain specific binding
phosphoprotein binding
Ras protein signal transduction
aging
insulin receptor signaling pathway
fibroblast growth factor receptor signaling pathway
positive regulation of signal transduction
cell differentiation
positive regulation of actin filament polymerization
receptor internalization
signal transduction in response to DNA damage
regulation of MAPK cascade
anatomical structure formation involved in morphogenesis
protein heterooligomerization
branching involved in labyrinthine layer morphogenesis
cellular response to ionizing radiation
positive regulation of reactive oxygen species metabolic process
MAPK cascade
epidermal growth factor receptor signaling pathway
cell-cell signaling
axon guidance
regulation of phosphatidylinositol 3-kinase signaling
viral process
T cell costimulation
phosphatidylinositol-3-phosphate biosynthetic process
Fc-epsilon receptor signaling pathway
Fc-gamma receptor signaling pathway involved in phagocytosis
ERBB2 signaling pathway
negative regulation of epidermal growth factor receptor signaling pathway
positive regulation of GTPase activity
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
leukocyte migration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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