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Invitrogen
GRK2 Polyclonal Antibody
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FIGURE: 1 / 5
GRK2 Antibody (PA5-77852) in WB
Western blot was performed using GRK2 Polyclonal Antibody (Product # PA5-77852) and a 80 kDa band corresponding to GRK2 was observed across cell lines and tissue extracts along with an uncharacterized band (*) at ~140 kDa in the cell lines tested. Whole cell extracts (30 µg lysate) of HEK293 (Lane 1), MCF7 (Lane 2), Jurkat (Lane 3), THP1 (Lane 4), tissue extracts (30 µg lysate) of Mouse Cerebellum (Lane 5), Mouse Spleen (Lane 6) and Mouse Heart (Lane 7) of Fig a; and MCF7 (Lane 1), MCF10A (Lane 2), Mouse Spleen (Lane 3) and Mouse Heart... View More
Product Details
PA5-77852
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GRK2. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.212 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2735885
Product Specific Information
Positive Control: Jurkat, Raji, NCI-H929
Predicted Reactivity: Mouse (100%), Rat (100%), Pig (100%), Bovine (100%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ADRBK1 (GRK2) is a serine/threonine kinase. Many of the G protein-coupled receptors are known to be phosphorylated by G protein-dependent receptor kinases (GRKs). G protein-coupled receptor kinases (GRKs) are important regulators of G protein-coupled receptors(GPCRs). GRK2 (83 kDa), one of six members of this family that have been identified, is ubiquitously expressed in mammals. After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs (e.g., a2-adrenergic receptor) are phosphorylated by GRKs. Internalization of the GPCRs, regulated by beta-arrestin-1, leads to activation of the Ras - Raf - ERK1&2 signaling pathway. GRK2 activity is tightly controlled by different mechanisms including phosphorylation by kinases such as PKC, Src and ERK1&2, as well as interaction with various proteins. ERK phosphorylates and thus inactivates GRK2 on serine 670 in a negative feedback mechanism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: adrenergic beta receptor kinase 1; adrenergic, beta, receptor kinase 1; Beta-adrenergic receptor kinase 1; beta-AR kinase-1; Beta-ARK-1; EC 2.7.1.126; G-protein coupled receptor kinase 2; G-protein-coupled receptor kinase 2; grk 2
Gene Aliases: Adrbk-1; ADRBK1; BARK; Bark-1; BARK1; beta ARK; BETA-ARK1; betaARK1; GRK2
UniProt ID: (Mouse) Q99MK8, (Human) P25098
Entrez Gene ID: (Mouse) 110355, (Human) 156
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
G-protein coupled receptor kinase activity
protein binding
ATP binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Edg-2 lysophosphatidic acid receptor binding
beta-adrenergic receptor kinase activity
alpha-2A adrenergic receptor binding
regulation of the force of heart contraction
desensitization of G-protein coupled receptor protein signaling pathway
negative regulation of the force of heart contraction by chemical signal
protein phosphorylation
response to oxidative stress
signal transduction
G-protein coupled receptor signaling pathway
tachykinin receptor signaling pathway
heart development
response to organic cyclic compound
phosphorylation
peptidyl-serine phosphorylation
peptidyl-threonine phosphorylation
viral genome replication
receptor internalization
positive regulation of catecholamine secretion
termination of G-protein coupled receptor signaling pathway
follicle-stimulating hormone signaling pathway
negative regulation of G-protein coupled receptor protein signaling pathway
negative regulation of striated muscle contraction
viral entry into host cell
cardiac muscle contraction
G-protein coupled acetylcholine receptor signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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