Proteintech

GRP75 Polyclonal Antibody

GRP75 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 15

GRP75 Antibody (14887-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells using 14887-1-AP (GRP75 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IGG.

GRP75 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

GRP75 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

14887-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 20 publications 20 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 5 publications 5 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 2 publications 2 publications

Not applicable (N/A)

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

GRP75 Fusion Protein Ag6673 (331-679 aa encoded by BC000478)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.23 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody recognized the endogenous HSPA9 protein in NIH/3T3 cells.

Immunogen sequence: YLTMDSSGP KHLNMKLTRA QFEGIVTDLI RRTIAPCQKA MQDAEVSKSD IGEVILVGGM TRMPKVQQTV QDLFGRAPSK AVNPDEAVAI GAAIQGGVLA GDVTDVLLLD VTPLSLGIET LGGVFTKLIN RNTTIPTKKS QVFSTAADGQ TQVEIKVCQG EREMAGDNKL LGQFTLIGIP PAPRGVPQIE VTFDIDANGI VHVSAKDKGT GREQQIVIQS SGGLSKDDIE NMVKNAEKYA EEDRRKKERV EAVNMAEGII HDTETKMEEF KDQLPADECN KLKEEISKMR ELLARKDSET GENIRQAASS LQQASLKLFE MAYKKMASER EGSGSSGTGE QKEDQKEEKQ (331-679 aa encoded by BC000478)

Target Information

The HSP70 family is composed of four highly conserved proteins: HSP70, HSC70, GRP75 and GRP78. These proteins serve a variety of roles. GRP75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP75 and GRP78 are unresponsive to heat stress and are induced by glucose deprivation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TOM complex-independent transport pathway of myoglobin into mitochondria in C2C12 myotubes.

Physiological reports

Koma R,Shibaguchi T,Araiso Y,Yamada T,Nonaka Y,Jue T,Masuda K

Sat Apr 01 00:00:00 UTC 2023

Pathological convergence of APP and SNCA deficiency in hippocampal degeneration of young rats.

Cell death & disease

Wang Y,Miao Z,Xu C,Cai Y,Yang Y,Hu Y,Zhao M,Shao Y,Li Z,Chen J,Chen S,Wang L

Sat May 13 00:00:00 UTC 2023

Development of a Novel Circulating Autoantibody Biomarker Panel for the Identification of Patients with 'Actionable' Pulmonary Nodules.

Cancers

Auger C,Moudgalya H,Neely MR,Stephan JT,Tarhoni I,Gerard D,Basu S,Fhied CL,Abdelkader A,Vargas M,Hu S,Hulett T,Liptay MJ,Shah P,Seder CW,Borgia JA

Wed Apr 12 00:00:00 UTC 2023

Potent ClpP agonists with anticancer properties bind with improved structural complementarity and alter the mitochondrial N-terminome.

Structure (London, England : 1993)

Mabanglo MF,Wong KS,Barghash MM,Leung E,Chuang SHW,Ardalan A,Majaesic EM,Wong CJ,Zhang S,Lang H,Karanewsky DS,Iwanowicz AA,Graves LM,Iwanowicz EJ,Gingras AC,Houry WA

Thu Feb 02 00:00:00 UTC 2023

The p97-UBXD8 complex regulates ER-Mitochondria contact sites by altering membrane lipid saturation and composition.

Nature communications

Ganji R,Paulo JA,Xi Y,Kline I,Zhu J,Clemen CS,Weihl CC,Purdy JG,Gygi SP,Raman M

Mon Feb 06 00:00:00 UTC 2023

Role of human HSPE1 for OPA1 processing independent of HSPD1.

iScience

Yeung N,Murata D,Iijima M,Sesaki H

Fri Feb 17 00:00:00 UTC 2023

Disruption of Mitochondrial-associated ER membranes by HIV-1 tat protein contributes to premature brain aging.

CNS neuroscience & therapeutics

Arjona SP,Allen CNS,Santerre M,Gross S,Soboloff J,Booze R,Sawaya BE

Sun Jan 01 00:00:00 UTC 2023

Disruption of mitochondria-associated ER membranes impairs insulin sensitivity and thermogenic function of adipocytes.

Frontiers in cell and developmental biology

Wang CH,Wang CH,Hung PJ,Wei YH

Wed Sep 28 00:00:00 UTC 2022

Disruption of mitochondria-associated ER membranes impairs insulin sensitivity and thermogenic function of adipocytes.

Frontiers in cell and developmental biology

Wang CH,Wang CH,Hung PJ,Wei YH

Wed Sep 28 00:00:00 UTC 2022

GRP75-driven, cell-cycle-dependent macropinocytosis of Tat/pDNA-Ca²⁺ nanoparticles underlies distinct gene therapy effect in ovarian cancer.

Journal of nanobiotechnology

Su L,Sun Z,Qi F,Su H,Qian L,Li J,Zuo L,Huang J,Yu Z,Li J,Chen Z,Zhang S

Wed Jul 20 00:00:00 UTC 2022

GRP75-driven, cell-cycle-dependent macropinocytosis of Tat/pDNA-Ca²⁺ nanoparticles underlies distinct gene therapy effect in ovarian cancer.

Journal of nanobiotechnology

Su L,Sun Z,Qi F,Su H,Qian L,Li J,Zuo L,Huang J,Yu Z,Li J,Chen Z,Zhang S

Wed Jul 20 00:00:00 UTC 2022

AMPK-dependent phosphorylation of MTFR1L regulates mitochondrial morphology.

Science advances

Tilokani L,Russell FM,Hamilton S,Virga DM,Segawa M,Paupe V,Gruszczyk AV,Protasoni M,Tabara LC,Johnson M,Anand H,Murphy MP,Hardie DG,Polleux F,Prudent J

Fri Nov 11 00:00:00 UTC 2022

Allosteric activation of Hsp70 reduces mutant huntingtin levels, the clustering of N-terminal fragments, and their nuclear accumulation.

Life sciences

Pinho BR,Almeida LM,Duchen MR,Oliveira JMA

Mon Nov 15 00:00:00 UTC 2021

LONP1 and mtHSP70 cooperate to promote mitochondrial protein folding.

Nature communications

Shin CS,Meng S,Garbis SD,Moradian A,Taylor RW,Sweredoski MJ,Lomenick B,Chan DC

Mon Jan 11 00:00:00 UTC 2021

A Mitochondrial DNA Variant Elevates the Risk of Gallstone Disease by Altering Mitochondrial Function.

Cellular and molecular gastroenterology and hepatology

Sun D,Niu Z,Zheng HX,Wu F,Jiang L,Han TQ,Wei Y,Wang J,Jin L

Mon Mar 07 00:00:00 UTC 2022

N-Propargylglycine: a unique suicide inhibitor of proline dehydrogenase with anticancer activity and brain-enhancing mitohormesis properties.

Amino acids

Scott GK,Mahoney S,Scott M,Loureiro A,Lopez-Ramirez A,Tanner JJ,Ellerby LM,Benz CC

Wed Dec 01 00:00:00 UTC 2021

N-Propargylglycine: a unique suicide inhibitor of proline dehydrogenase with anticancer activity and brain-enhancing mitohormesis properties.

Amino acids

Scott GK,Mahoney S,Scott M,Loureiro A,Lopez-Ramirez A,Tanner JJ,Ellerby LM,Benz CC

Wed Dec 01 00:00:00 UTC 2021

Mitochondrial DNA Haplogroup M7 Confers Disability in a Chinese Aging Population.

Frontiers in genetics

Sun D,Yao S,Wu F,Deng W,Ma Y,Jin L,Wang J,Wang X

Tue Nov 17 00:00:00 UTC 2020

ATF4 Mediates Mitochondrial Unfolded Protein Response in Alveolar Epithelial Cells.

American journal of respiratory cell and molecular biology

Jiang D,Cui H,Xie N,Banerjee S,Liu RM,Dai H,Thannickal VJ,Liu G

Thu Oct 01 00:00:00 UTC 2020

ATF4 Mediates Mitochondrial Unfolded Protein Response in Alveolar Epithelial Cells.

American journal of respiratory cell and molecular biology

Jiang D,Cui H,Xie N,Banerjee S,Liu RM,Dai H,Thannickal VJ,Liu G

Thu Oct 01 00:00:00 UTC 2020

Excess hydrogen sulfide and polysulfides production underlies a schizophrenia pathophysiology.

EMBO molecular medicine

Ide M,Ohnishi T,Toyoshima M,Balan S,Maekawa M,Shimamoto-Mitsuyama C,Iwayama Y,Ohba H,Watanabe A,Ishii T,Shibuya N,Kimura Y,Hisano Y,Murata Y,Hara T,Morikawa M,Hashimoto K,Nozaki Y,Toyota T,Wada Y,Tanaka Y,Kato T,Nishi A,Fujisawa S,Okano H,Itokawa M,Hirokawa N,Kunii Y,Kakita A,Yabe H,Iwamoto K,Meno K,Katagiri T,Dean B,Uchida K,Kimura H,Yoshikawa T

Sun Dec 01 00:00:00 UTC 2019

TDP-43 induces mitochondrial damage and activates the mitochondrial unfolded protein response.

PLoS genetics

Wang P,Deng J,Dong J,Liu J,Bigio EH,Mesulam M,Wang T,Sun L,Wang L,Lee AY,McGee WA,Chen X,Fushimi K,Zhu L,Wu JY

Wed May 01 00:00:00 UTC 2019

TP53-induced glycolysis and apoptosis regulator is indispensable for mitochondria quality control and degradation following damage.

Oncology letters

Feng J,Luo L,Liu Y,Fu S,Chen J,Duan X,Xiang L,Zhang Y,Wu J,Fan J,Wen Q,Zhang Y,Yang J,Peng J,Zhao M,Yang L

Mon Jan 01 00:00:00 UTC 2018

TP53-induced glycolysis and apoptosis regulator is indispensable for mitochondria quality control and degradation following damage.

Oncology letters

Feng J,Luo L,Liu Y,Fu S,Chen J,Duan X,Xiang L,Zhang Y,Wu J,Fan J,Wen Q,Zhang Y,Yang J,Peng J,Zhao M,Yang L

Mon Jan 01 00:00:00 UTC 2018

TP53-induced glycolysis and apoptosis regulator is indispensable for mitochondria quality control and degradation following damage.

Oncology letters

Feng J,Luo L,Liu Y,Fu S,Chen J,Duan X,Xiang L,Zhang Y,Wu J,Fan J,Wen Q,Zhang Y,Yang J,Peng J,Zhao M,Yang L

Mon Jan 01 00:00:00 UTC 2018

TP53-induced glycolysis and apoptosis regulator is indispensable for mitochondria quality control and degradation following damage.

Oncology letters

Feng J,Luo L,Liu Y,Fu S,Chen J,Duan X,Xiang L,Zhang Y,Wu J,Fan J,Wen Q,Zhang Y,Yang J,Peng J,Zhao M,Yang L

Mon Jan 01 00:00:00 UTC 2018

FAM210B is an erythropoietin target and regulates erythroid heme synthesis by controlling mitochondrial iron import and ferrochelatase activity.

The Journal of biological chemistry

Yien YY,Shi J,Chen C,Cheung JTM,Grillo AS,Shrestha R,Li L,Zhang X,Kafina MD,Kingsley PD,King MJ,Ablain J,Li H,Zon LI,Palis J,Burke MD,Bauer DE,Orkin SH,Koehler CM,Phillips JD,Kaplan J,Ward DM,Lodish HF,Paw BH

Fri Dec 21 00:00:00 UTC 2018

FAM210B is an erythropoietin target and regulates erythroid heme synthesis by controlling mitochondrial iron import and ferrochelatase activity.

The Journal of biological chemistry

Fri Dec 21 00:00:00 UTC 2018

LONP1 Is Required for Maturation of a Subset of Mitochondrial Proteins, and Its Loss Elicits an Integrated Stress Response.

Molecular and cellular biology

Zurita Rendón O,Shoubridge EA

Mon Oct 15 00:00:00 UTC 2018

Loss of OMA1 delays neurodegeneration by preventing stress-induced OPA1 processing in mitochondria.

The Journal of cell biology

Korwitz A,Merkwirth C,Richter-Dennerlein R,Tröder SE,Sprenger HG,Quirós PM,López-Otín C,Rugarli EI,Langer T

Mon Jan 18 00:00:00 UTC 2016

Loss of OMA1 delays neurodegeneration by preventing stress-induced OPA1 processing in mitochondria.

The Journal of cell biology

Korwitz A,Merkwirth C,Richter-Dennerlein R,Tröder SE,Sprenger HG,Quirós PM,López-Otín C,Rugarli EI,Langer T

Mon Jan 18 00:00:00 UTC 2016

ABT737 reverses cisplatin resistance by regulating ER-mitochondria Ca2+ signal transduction in human ovarian cancer cells.

International journal of oncology

Xie Q,Su J,Jiao B,Shen L,Ma L,Qu X,Yu C,Jiang X,Xu Y,Sun L

Thu Dec 01 00:00:00 UTC 2016

ABT737 reverses cisplatin resistance by regulating ER-mitochondria Ca2+ signal transduction in human ovarian cancer cells.

International journal of oncology

Xie Q,Su J,Jiao B,Shen L,Ma L,Qu X,Yu C,Jiang X,Xu Y,Sun L

Thu Dec 01 00:00:00 UTC 2016

DNAJC19, a mitochondrial cochaperone associated with cardiomyopathy, forms a complex with prohibitins to regulate cardiolipin remodeling.

Cell metabolism

Richter-Dennerlein R,Korwitz A,Haag M,Tatsuta T,Dargazanli S,Baker M,Decker T,Lamkemeyer T,Rugarli EI,Langer T

Tue Jul 01 00:00:00 UTC 2014

DNAJC19, a mitochondrial cochaperone associated with cardiomyopathy, forms a complex with prohibitins to regulate cardiolipin remodeling.

Cell metabolism

Richter-Dennerlein R,Korwitz A,Haag M,Tatsuta T,Dargazanli S,Baker M,Decker T,Lamkemeyer T,Rugarli EI,Langer T

Tue Jul 01 00:00:00 UTC 2014

Upregulation of phosphorylated HSP27, PRDX2, GRP75, GRP78 and GRP94 in acquired middle ear cholesteatoma growth.

International journal of molecular sciences

Ho KY,Yeh TS,Huang HH,Hung KF,Chai CY,Chen WT,Tsai SM,Chang NC,Chien CY,Wang HM,Wu YJ

Thu Jul 11 00:00:00 UTC 2013

Upregulation of phosphorylated HSP27, PRDX2, GRP75, GRP78 and GRP94 in acquired middle ear cholesteatoma growth.

International journal of molecular sciences

Ho KY,Yeh TS,Huang HH,Hung KF,Chai CY,Chen WT,Tsai SM,Chang NC,Chien CY,Wang HM,Wu YJ

Thu Jul 11 00:00:00 UTC 2013

An investigation into the cytotoxic effects of 13-acetoxysarcocrassolide from the soft coral Sarcophyton crassocaule on bladder cancer cells.

Marine drugs

Su CC,Su JH,Lin JJ,Chen CC,Hwang WI,Huang HH,Wu YJ

Thu Dec 01 00:00:00 UTC 2011

An investigation into the cytotoxic effects of 13-acetoxysarcocrassolide from the soft coral Sarcophyton crassocaule on bladder cancer cells.

Marine drugs

Su CC,Su JH,Lin JJ,Chen CC,Hwang WI,Huang HH,Wu YJ

Thu Dec 01 00:00:00 UTC 2011

Bioinformatics

Protein Aliases: 75 kDa glucose-regulated protein; C3H-specific antigen; catecholamine-regulated protein 40; epididymis secretory sperm binding protein Li 124m; GRP 75; GRP-75; Heat shock 70 kDa protein 9; heat shock 70kD protein 9B; heat shock 70kDa protein 9 (mortalin); heat shock 70kDa protein 9A; Heat shock protein; heat shock protein 9A; heat shock protein cognate 74; Heat shock protein family A member 9; heat shock protein, 74 kDa, A; heat shock protein, A; HSP; MGC4500; Mortalin; mortalin, perinuclear; mortalin-2; mortalin2; MOT; mtHSP70; p66 MOT; p66-mortalin; PBP74; Peptide-binding protein 74; Stress-70 protein, mitochondrial

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Gene Aliases: 74kDa; CRP40; CSA; EVPLS; GRP-75; GRP75; HEL-S-124m; Hsc74; Hsp74; Hsp74a; HSPA9; Hspa9a; HSPA9B; Mortalin; MOT; Mot-2; MOT2; mt-HSP70; Mthsp70; MTHSP75; PBP74; SAAN; SIDBA4

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UniProt ID: (Human) P38646, (Mouse) P38647

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Entrez Gene ID: (Human) 3313, (Rat) 291671, (Mouse) 15526

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Function(s)

protein binding ATP binding fibroblast growth factor binding heat shock protein binding ubiquitin protein ligase binding poly(A) RNA binding unfolded protein binding enzyme binding nucleotide binding

Process(es)

protein folding protein export from nucleus response to toxic substance cellular response to interleukin-1 erythrocyte differentiation negative regulation of apoptotic process negative regulation of erythrocyte differentiation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GRP75 Polyclonal Antibody

GRP75 Antibody (14887-1-AP) in ICC/IF

Product Details

14887-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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