Invitrogen

GRP78 Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-29705

Applications
Tested Dilution
Publications

Western Blot (WB)

1:5,000-1:20,000
View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-
Product Specifications

Species Reactivity

Chicken, Human, Mouse, Rat

Published species

Human, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Grp78. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.56 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2547179

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, HepG2, NIH-3T3, JC, BCL-1, PC-12, Rat2.

Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (95%), Japanese Medaka (95%), Xenopus laevis (99%), Chicken (99%), Rhesus Monkey (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

GRP78 is a 78 kDa glucose regulated protein that belongs to the family of heat shock proteins that include HSP70. GRP78 is a resident protein of the endoplasmic reticulum (ER) and associates transiently with a variety of newly synthesized secretory and membrane proteins. GRP78 may also interact with mutant and misfolded proteins, marking them for degradation. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C-terminus of GRP78 and other resident ER proteins including glucose regulated protein 94 (GRP94) and protein disulfide isomerase (PDI). The KDEL signal is recognized by the KDEL receptor and this interaction ensures recycling of escaped ER proteins back to the ER. GRP78’s involvement in correct folding of proteins and degradation of misfolded proteins is via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate. GRP78 is critical for maintenance of cell homeostasis and the prevention of apoptosis. GRP78 protein levels have been shown to be a reliable biomarker of hypoglycemia and serves a neuroprotective function in neurons exposed to glutamate and oxidative stress. GRP78 levels are reduced in the brains of Alzheimer's Disease patients, and decreased expression of GRP78 is found associated with missense mutations in the human presenilin-1 (PS1) gene. Further, the induction of the GRP78 protein has been associated with the development of drug-resistance to antitumor drugs.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 78 kDa glucose-regulated protein; Binding-immunoglobulin protein; BiP; Endoplasmic reticulum chaperone BiP; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; epididymis secretory sperm binding protein Li 89n; FLJ26106; glucose regulated protein, 78 kDa; glucose-regulated protein, 78kDa; GRP 78; GRP-78; heat shock 70 kDa protein 5; heat shock 70kD protein 5; heat shock 70kD protein 5 (glucose-regulated protein, 78kD); heat shock 70kDa protein 5 (glucose-regulated protein); heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); Heat shock protein 70 family protein 5; Heat shock protein family A member 5; HSP70 family protein 5; HSPA 5; Immunoglobulin heavy chain-binding protein; Steroidogenesis-activator polypeptide; XAP-1 antigen

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Gene Aliases: AL022860; AU019543; baffled; BIP; D2Wsu141e; D2Wsu17e; GRP78; HEL-S-89n; Hsce70; HSPA5; mBiP; MIF2; SEZ-7; Sez7

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UniProt ID: (Chicken) Q90593, (Human) P11021, (Rat) P06761, (Mouse) P20029

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Entrez Gene ID: (Chicken) 396487, (Human) 3309, (Rat) 25617, (Mouse) 14828

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Function(s)
protein binding ATP binding protein domain specific binding ubiquitin protein ligase binding ribosome binding misfolded protein binding glycoprotein binding calcium ion binding ATPase activity enzyme binding unfolded protein binding chaperone binding cadherin binding involved in cell-cell adhesion nucleotide binding
Process(es)
ER overload response activation of signaling protein activity involved in unfolded protein response cerebellum structural organization cerebellar Purkinje cell layer development substantia nigra development positive regulation of cell migration negative regulation of transforming growth factor beta receptor signaling pathway positive regulation of protein ubiquitination response to endoplasmic reticulum stress maintenance of protein localization in endoplasmic reticulum cellular response to glucose starvation negative regulation of apoptotic process proteolysis involved in cellular protein catabolic process cellular response to antibiotic cellular response to interleukin-4 toxin transport positive regulation of embryonic development ER-associated ubiquitin-dependent protein catabolic process endoplasmic reticulum unfolded protein response protein folding in endoplasmic reticulum IRE1-mediated unfolded protein response PERK-mediated unfolded protein response ATF6-mediated unfolded protein response regulation of protein folding in endoplasmic reticulum cellular response to manganese ion negative regulation of protein homodimerization activity cell-cell adhesion regulation of ATF6-mediated unfolded protein response regulation of IRE1-mediated unfolded protein response regulation of PERK-mediated unfolded protein response positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress
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