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FIGURE: 1 / 4
GSDMC Antibody (MA5-51281) in WB
Western blot analysis of GSDMC in extracts of various cell lines. Samples were incubated with GSDMC Recombinant monoclonal antibody (Product # MA5-51281) using a dilution of 1:10,000 followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
MA5-51281
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
1G8H5
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-150 of human GSDMC (NP_113603.1).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_3094312
Product Specific Information
Immunogen sequence: MPSMLERISK NLVKEIGSKD LTPVKYLLSA TKLRQFVILR KKKDSRSSFW EQSDYVPVEF SLNDILEPSS SVLETVVTGP FHFSDIMIQK HKADMGVNVG IEVSVSGEAS VDHGCSLEFQ IVTIPSPNLE DFQKRKLLDP EPSFLKECRR
Target Information
The N-terminal moiety promotes pyroptosis. May be acting by homooligomerizing within the membrane and forming pores (PubMed:27281216). The physiological relevance of this observation is unknown (Probable). [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: gasdermin C1; Gasdermin-C; Gasdermin-C1; melanoma-derived leucine zipper, extra-nuclear factor; Melanoma-derived leucine zipper-containing extranuclear factor; melanoma-derived leucine zipper-containing extranuclear factor {ECO:0000250|UniProtKB:Q9BYG8}; mMLZE
Gene Aliases: GSDMC; Gsdmc1; MLZE
UniProt ID: (Human) Q9BYG8, (Mouse) Q99NB5
Entrez Gene ID: (Human) 56169, (Mouse) 83492, (Rat) 299908
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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