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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • GSK3B Antibodies

          Proteintech

          GSK3B Polyclonal Antibody

          4 References
          View all (84) GSK3B antibodies

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          Datasheet
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          Cite GSK3B Polyclonal Antibody

          • Antibody Testing Data (9)
          GSK3B Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          GSK3B Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 9

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          GSK3B Antibody (24198-1-AP) in ICC/IF

          Immunofluorescent analysis of Ethacrynic acid treated HeLa cells using 24198-1-AP (GSK3B antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IGG. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          GSK3B Antibody in Immunocytochemistry (ICC/IF)
          GSK3B Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          GSK3B Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          GSK3B Antibody in Western Blot (WB)
          GSK3B Antibody in Western Blot (WB)
          GSK3B Antibody in Western Blot (WB)
          GSK3B Antibody in Western Blot (WB)
          GSK3B Antibody in Flow Cytometry (Flow)
          GSK3B Antibody in Immunoprecipitation (IP)

          Product Details

          24198-1-AP

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          View 3 publications 3 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:20-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:10-1:100
          -

          Flow Cytometry (Flow)

          0.05 µg/1x10^6 cells
          -

          Immunoprecipitation (IP)

          0.5-4.0 µg
          -

          Not applicable (N/A)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          GSK3B Fusion Protein Ag21438 (1-433 aa encoded by BC000251)

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.35 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          Immunogen sequence: MSGRPRTTS FAESCKPVQQ PSAFGSMKVS RDKDGSKVTT VVATPGQGPD RPQEVSYTDT KVIGNGSFGV VYQAKLCDSG ELVAIKKVLQ DKRFKNRELQ IMRKLDHCNI VRLRYFFYSS GEKKDEVYLN LVLDYVPETV YRVARHYSRA KQTLPVIYVK LYMYQLFRSL AYIHSFGICH RDIKPQNLLL DPDTAVLKLC DFGSAKQLVR GEPNVSYICS RYYRAPELIF GATDYTSSID VWSAGCVLAE LLLGQPIFPG DSGVDQLVEI IKVLGTPTRE QIREMNPNYT EFKFPQIKAH PWTKDSSGTG HFTSGVRVFR PRTPPEAIAL CSRLLEYTPT ARLTPLEACA HSFFDELRDP NVKLPNGRDT PALFNFTTQE LSSNPPLATI LIPPHARIQA AASTPTNATA ASDANTGDRG QTNNAASASA SNST (1-433 aa encoded by BC000251)

          Target Information

          GSK3B is a proline-directed serine/threonine protein kinase involved in energy metabolism, neuronal cell development and body pattern formation. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. GSK3B is a critical component of the Wnt/beta-catenin signaling pathway, and its activity results in the inhibition of Wnt signaling by mediating the degradation of beta-catenin. GSK3B is also involved in the Hedgehog, Notch, and insulin signaling pathways.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: EC 2.7.11.26; Glycogen synthase kinase-3 beta; GSK 3 beta; GSK 3B; GSK-3 beta; GSK-3 beta GSK3B; GSK-3b; GSK3 beta; GSK3beta isoform; pp37; Serine/threonine-protein kinase GSK3B

          View more View less

          Gene Aliases: 7330414F15Rik; 8430431H08Rik; C86142; GSK-3; GSK-3beta; GSK3; GSK3B

          View more View less

          UniProt ID: (Human) P49841, (Mouse) Q9WV60

          View more View less

          Entrez Gene ID: (Human) 2932, (Mouse) 56637

          View more View less

          Function(s)
          RNA polymerase II transcription factor binding p53 binding protein kinase activity protein serine/threonine kinase activity integrin binding protein binding ATP binding beta-catenin binding kinase activity protein kinase binding ubiquitin protein ligase binding protein kinase A catalytic subunit binding ionotropic glutamate receptor binding tau protein binding tau-protein kinase activity NF-kappaB binding nucleotide binding transcription factor binding transferase activity transferase activity, transferring phosphorus-containing groups
          Process(es)
          re-entry into mitotic cell cycle epithelial to mesenchymal transition positive regulation of cell-matrix adhesion carbohydrate metabolic process glycogen metabolic process regulation of gene expression by genetic imprinting protein phosphorylation protein export from nucleus ER overload response cytoskeleton organization establishment or maintenance of cell polarity multicellular organism development nervous system development axonogenesis myoblast fusion circadian rhythm cell proliferation organ morphogenesis negative regulation of signal transduction negative regulation of cardiac muscle hypertrophy positive regulation of peptidyl-threonine phosphorylation regulation of neuron projection development negative regulation of neuron projection development negative regulation of neuron maturation myotube differentiation Wnt signaling pathway phosphorylation cell migration peptidyl-serine phosphorylation hippocampus development establishment of cell polarity cell differentiation negative regulation of protein complex assembly positive regulation of protein complex assembly negative regulation of protein binding positive regulation of protein binding positive regulation of proteasomal ubiquitin-dependent protein catabolic process regulation of microtubule-based process positive regulation of peptidyl-serine phosphorylation protein localization to microtubule intracellular signal transduction cellular response to interleukin-3 positive regulation of apoptotic process negative regulation of apoptotic process negative regulation of MAP kinase activity positive regulation of GTPase activity hypermethylation of CpG island canonical Wnt signaling pathway involved in positive regulation of apoptotic process fat cell differentiation positive regulation of axon extension positive regulation of transcription from RNA polymerase II promoter protein autophosphorylation positive regulation of protein export from nucleus regulation of neuronal synaptic plasticity rhythmic process negative regulation of dendrite morphogenesis negative regulation of NFAT protein import into nucleus canonical Wnt signaling pathway superior temporal gyrus development cellular response to mechanical stimulus extrinsic apoptotic signaling pathway in absence of ligand positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway positive regulation of stem cell differentiation dopamine receptor signaling pathway aging positive regulation of mitochondrial membrane potential response to activity peptidyl-threonine phosphorylation response to estradiol response to insulin negative regulation of smooth muscle cell apoptotic process cellular response to hepatocyte growth factor stimulus cellular response to erythropoietin response to drug proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of neuron apoptotic process negative regulation of glycogen biosynthetic process positive regulation of protein catabolic process positive regulation of smooth muscle cell proliferation negative regulation of nitric-oxide synthase activity intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress cellular response to hydrogen peroxide cellular response to iron(II) ion cellular response to lithium ion response to epinephrine negative regulation of canonical Wnt signaling pathway chemical synaptic transmission, postsynaptic regulation of cellular response to heat negative regulation of protein localization to nucleus positive regulation of neuron death response to L-glutamate cellular response to dopamine negative regulation of dopaminergic neuron differentiation beta-catenin destruction complex assembly beta-catenin destruction complex disassembly response to insulin-like growth factor stimulus response to ultrasound response to angiotensin negative regulation of type B pancreatic cell development positive regulation of excitatory postsynaptic potential negative regulation of glycogen (starch) synthase activity positive regulation of DNA biosynthetic process negative regulation of neuron migration
          It has to be done as per old AB suggested Products section.
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