Invitrogen

GSPT1 Polyclonal Antibody

GSPT1 Antibody in Immunohistochemistry (IHC)

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GSPT1 Antibody (PA5-62621) in IHC

Immunohistochemical staining of GSPT1 in human testis using a GSPT1 Polyclonal Antibody (Product # PA5-62621) shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.

Product Image Gallery

GSPT1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. Hep-G2 cells were transfected with, control siRNA, GSPT1 siRNA probe #1, and GSPT1 siRNA probe #2 and a decrease in signal intensity was observed in Western Blot application using Anti-GSPT1 Polyclonal Antibody (Product # PA5-62621) used at a dilution of 0.4 µg/mL. Anti-GAPDH was used as a loading control. {KD}

Product Details

PA5-62621

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human GSPT1. Recombinant protein control fragment (Product #RP-98657).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.14 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2642244

Product Specific Information

Immunogen sequence: GGAANNHGAG SGAGGRAAPV ESSQEEQSLC EGSNSAVS

Highest antigen sequence identity to the following orthologs: Mouse - 66%, Rat - 74%.

Target Information

eRF3a (eukaryotic peptide chain release factor subunit 3a), also known as GSPT1 (G1 to S phase transition 1), is a 499 amino acid protein that belongs to the GTP-binding elongation factor family and is involved in the regulation of cell growth, specifically via control of translation termination. Human eRF3a shares 94% sequence identity with its mouse counterpart, suggesting a conserved function between species. The gene encoding eRF3a maps to human chromosome 16, which encodes over 900 genes and comprises nearly 3% of the human genome. The GAN gene is located on chromosome 16 and, with mutation, may lead to giant axonal neuropathy, a nervous system disorder characterized by increasing malfunction with growth.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Pharmacological induction of translational readthrough of nonsense mutations in the retinoblastoma (RB1) gene.

PloS one

Palomar-Siles M,Yurevych V,Bykov VJN,Wiman KG

PA5-62621 was used in Western Blot to suggest that pharmacological induction of translational readthrough could be a feasible strategy for therapeutic targeting of tumors with nonsense mutant RB1.

Mon Nov 06 00:00:00 UTC 2023

Pharmacological induction of translational readthrough of nonsense mutations in the retinoblastoma (RB1) gene.

PloS one

Palomar-Siles M,Yurevych V,Bykov VJN,Wiman KG

PA5-62621 was used in Western Blot to suggest that pharmacological induction of translational readthrough could be a feasible strategy for therapeutic targeting of tumors with nonsense mutant RB1.

Mon Nov 06 00:00:00 UTC 2023

Effect of small molecule eRF3 degraders on premature termination codon readthrough.

Nucleic acids research

Baradaran-Heravi A,Balgi AD,Hosseini-Farahabadi S,Choi K,Has C,Roberge M

PA5-62621 was used in Simple Western - Size to show that PTC readthrough by aminoglycoside G418 is considerably enhanced by eRF3a and eRF3b siRNAs and cereblon E3 ligase modulators CC-885 and CC-90009, which induce proteasomal degradation of eRF3a and eRF3b.

Mon Apr 19 00:00:00 UTC 2021

Effect of small molecule eRF3 degraders on premature termination codon readthrough.

Nucleic acids research

Baradaran-Heravi A,Balgi AD,Hosseini-Farahabadi S,Choi K,Has C,Roberge M

Mon Apr 19 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: Eukaryotic peptide chain release factor GTP-binding subunit ERF3A; Eukaryotic peptide chain release factor subunit 3a; G1 to S phase transition protein 1 homolog

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Gene Aliases: 551G9.2; ERF3A; ETF3A; GSPT1; GST1

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UniProt ID: (Human) P15170

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Entrez Gene ID: (Human) 2935

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Function(s)

translation release factor activity GTPase activity protein binding GTP binding poly(A) RNA binding

Process(es)

G1/S transition of mitotic cell cycle nuclear-transcribed mRNA catabolic process, nonsense-mediated decay cytoplasmic translational termination protein methylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GSPT1 Polyclonal Antibody

GSPT1 Antibody (PA5-62621) in IHC

Product Details

PA5-62621

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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