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Product Details
PA5-90339
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 230-439 of human GTF2E1 (NP_0055042)
Conjugate
Form
Liquid
Concentration
2.82 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2806033
Product Specific Information
Immunogen sequence: LAGGHHREAW ATKGPSYEDL YTQNVVINMD DQEDLHRASL EGKSAKERPI WLRESTVQGA YGSEDMKEGG IDMDAFQERE EGHAGPDDNE EVMRALLIHE KKTSSAMAGS VGAAAPVTAA NGSDSESETS ESDDDSPPRP AAVAVHKREE DEEEDDEFEE VADDPIVMVA GRPFSYSEVS QRPELVAQMT PEEKEAYIAM GQRMFEDLFE; Positive Samples: HeLa, Jurkat, A-431, Mouse brain, Mouse spleen, Mouse testis; Cellular Location: Nucleus
Target Information
The general transcription factor TFIIE has an essential role in eukaryotic transcription initiation together with RNA polymerase II and other general factors. Human TFIIE consists of two subunits of relative molecular mass 57, 000 (TFIIE-alpha) and 34, 000 (TFIIE-beta) and joins the preinitiation complex after RNA polymerase II and TFIIF. TFIIE-alpha is necessary for transcription initiation together with TFIIE-beta, and recombinant TFIIE-alpha can fully replace the natural subunit in an in vitro transcription assay. Its sequence contains several interesting structural motifs (leucine repeat, zinc finger and helix-turn-helix) and sequence similarities to bacterial sigma factors that suggest direct involvement in the regulation of transcription initiation.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: general transcription factor II E, polypeptide 1 (alpha subunit, 56kDa); General transcription factor IIE 56 kDa subunit; General transcription factor IIE subunit 1; general transcription factor IIE, polypeptide 1 (alpha subunit); general transcription factor IIE, polypeptide 1 (alpha subunit, 56kDa); general transcription factor IIE, polypeptide 1, alpha 56kDa; TFIIE alpha subunit; TFIIE-alpha; Transcription initiation factor IIE subunit alpha
Gene Aliases: 2610024P03Rik; 56kDa; AI303800; FE; GTF2E1; TF2E1; TFIIE-A
UniProt ID: (Human) P29083, (Mouse) Q9D0D5
Entrez Gene ID: (Human) 2960, (Rat) 303918, (Mouse) 74197
transcription initiation from RNA polymerase II promoter
biological_process
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
viral process
snRNA transcription from RNA polymerase II promoter
transcription, DNA-templated
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