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GTF2E1/TFIIE-alpha Antibody (A302-754A) in IHC (P)
Detection of human GTF2E1/TFIIE-alpha by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-GTF2E1/TFIIE-alpha (Product # A302-754A) (Lot 1) used at a dilution of 1:1,000 (1 µg/mL). Detection: DAB.
Product Details
A302-754A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 100 and 150 of human General Transcription Factor IIE, polypeptide 1, alpha,.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Based on 100% sequence identity, this antibody is predicted to react with Bovine and Orangutan
Target Information
The general transcription factor TFIIE has an essential role in eukaryotic transcription initiation together with RNA polymerase II and other general factors. Human TFIIE consists of two subunits of relative molecular mass 57, 000 (TFIIE-alpha) and 34, 000 (TFIIE-beta) and joins the preinitiation complex after RNA polymerase II and TFIIF. TFIIE-alpha is necessary for transcription initiation together with TFIIE-beta, and recombinant TFIIE-alpha can fully replace the natural subunit in an in vitro transcription assay. Its sequence contains several interesting structural motifs (leucine repeat, zinc finger and helix-turn-helix) and sequence similarities to bacterial sigma factors that suggest direct involvement in the regulation of transcription initiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: General transcription factor IIE 56 kDa subunit; General transcription factor IIE subunit 1; general transcription factor IIE, polypeptide 1, alpha 56kDa; TFIIE alpha subunit; TFIIE-alpha; Transcription initiation factor IIE subunit alpha
Gene Aliases: FE; GTF2E1; TF2E1; TFIIE-A
UniProt ID: (Human) P29083
Entrez Gene ID: (Human) 2960
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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