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GTF2H2 Antibody (PA5-104234) in ICC/IF
Immunocytochemistry analysis of GTF2H2 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with GTF2H2 polyclonal antibody (Product # PA5-104234) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-104234
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human GTF2H2(Accession Q13888), corresponding to amino acid residues M1-E48.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2853558
Product Specific Information
Antibody detects endogenous levels of total TF2H2.
Target Information
Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription factors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in the formation of a multiprotein complex containing several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFII. TFIIH (or BTF2) is a multisubunit transcription/DNA repair factor that possesses several enzymatic activities. The core of TFIIH is composed of five subunits, designated p89 (XPB or ERCC3), p62, p52, p44 and p34. Additional subunits of the TFIIH complex are p80 (XPD or ERCC2) and the ternary kinase complex composed of Cdk7, cyclin H and MAT1. Both p89 and p80 have ATP-dependent helicase activity. The p62, p52 and p44 subunits have been shown to be involved in nucleotide excision repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: basal transcription factor 2, p44 subunit; Basic transcription factor 2 44 kDa subunit; BTF2 p44; BTF2-p44; general transcription factor II H, polypeptide 2 (44 kDa subunit); General transcription factor IIH polypeptide 2; General transcription factor IIH subunit 2; general transcription factor IIH, polypeptide 2 (44 kDa subunit); general transcription factor IIH, polypeptide 2, 44kD subunit; general transcription factor IIH, polypeptide 2, 44kDa; TFIIH basal transcription factor complex p44 subunit
Gene Aliases: 44kDa; BTF2; BTF2 p44; BTF2-p44; BTF2P44; GTF2H2; p44; T-BTF2P44; TFIIH
UniProt ID: (Human) Q13888, (Mouse) Q9JIB4, (Rat) A0JN27
Entrez Gene ID: (Human) 2966, (Mouse) 23894, (Rat) 294693
nucleic acid binding
transcription factor activity, sequence-specific DNA binding
protein kinase activity
protein binding
DNA-dependent ATPase activity
translation factor activity, RNA binding
zinc ion binding
RNA polymerase II carboxy-terminal domain kinase activity
protein N-terminus binding
metal ion binding
molecular_function
nucleotide-excision repair, DNA duplex unwinding
G-protein coupled receptor internalization
transcription-coupled nucleotide-excision repair
nucleotide-excision repair, preincision complex stabilization
nucleotide-excision repair, preincision complex assembly
nucleotide-excision repair, DNA incision, 3'-to lesion
nucleotide-excision repair, DNA incision, 5'-to lesion
regulation of transcription, DNA-templated
transcription initiation from RNA polymerase I promoter
transcription elongation from RNA polymerase I promoter
termination of RNA polymerase I transcription
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
7-methylguanosine mRNA capping
translation
protein phosphorylation
response to UV
nucleotide-excision repair, DNA incision
global genome nucleotide-excision repair
DNA repair
nucleotide-excision repair
transcription, DNA-templated
cellular response to DNA damage stimulus
biological_process
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