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GTPBP4 Antibody (MA5-34853) in IHC (P)
Immunohistochemistry analysis of GTPBP4 in paraffin-embedded human thyroid gland tissue. Samples were heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, 20 min) and blocked in 5% BSA (30 min, room temperature), incubated with GTPBP4 monoclonal antibody (Product # MA5-34853) at a dilution of 1:50 (30 min, room temperature), and followed by HRP conjugated compact polymer system, DAB, counters... View More
Product Details
MA5-34853
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE47-47
Immunogen
Recombinant protein within Human GTPBP4 aa 1-131
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2848761
Product Specific Information
Positive Control: 293T cell, human liver cancer tissue, human thyroid gland tissue, human skin tissue, human breast tissue, thp-1.
Target Information
GTP-binding proteins are GTPases and function as molecular switches that can flip between two states: active, when GTP is bound, and inactive, when GDP is bound. 'Active' in this context usually means that the molecule acts as a signal to trigger other events in the cell. When an extracellular ligand binds to a G-protein-linked receptor, the receptor changes its conformation and switches on the trimeric G proteins that associate with it by causing them to eject their GDP and replace it with GTP. The switch is turned off when the G protein hydrolyzes its own bound GTP, converting it back to GDP. But before that occurs, the active protein has an opportunity to diffuse away from the receptor and deliver its message for a prolonged period to its downstream target.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Chronic renal failure gene protein; FLJ10686; FLJ10690; FLJ39774; G protein-binding protein CRFG; GTP-binding protein 4; GTP-binding protein NGB; Nucleolar GTP-binding protein 1
Gene Aliases: CRFG; GTPBP4; NGB; NOG1
UniProt ID: (Human) Q9BZE4
Entrez Gene ID: (Human) 23560
regulation of cyclin-dependent protein serine/threonine kinase activity
osteoblast differentiation
negative regulation of DNA replication
negative regulation of cell proliferation
negative regulation of cell-cell adhesion
negative regulation of cell migration
negative regulation of protein ubiquitination
negative regulation of collagen binding
ribosome biogenesis
protein stabilization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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