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Invitrogen
GUCY1B2 Polyclonal Antibody
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FIGURE: 1 / 2
GUCY1B2 Antibody (PA5-103135) in ICC/IF
Immunocytochemistry analysis of GUCY1B2 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with GUCY1B2 polyclonal antibody (Product # PA5-103135) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-103135
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human GUCY1B2(Accession O75343), corresponding to amino acid residues P581-L617.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2852505
Product Specific Information
Antibody detects endogenous levels of total GCS beta 2.
Target Information
Guanylate cyclases belong to the adenylyl cyclase class-4/guanylyl cyclase family. There are two forms of guanylate cyclase. The soluble form, known as GCS or sGC, act as receptors for nitric oxide. The membrane-bound receptor form, known as GC, are peptide hormone receptors. GCS is a cGMP-synthesizing enzyme, which is the major receptor for the neurotransmitter nitric oxide (NO). It plays a crucial role in smooth muscle contractility, platelet reactivity and neurotransmission. GCS is a heme containing heterodimer, consisting of one 72 kDa alpha subunit and one 65 kDa beta subunit. The heme moeity mediates NO activation, and this heme group also binds carbon monoxide, which weakly stimulates the enzyme.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GCS beta2; GCS-beta-2; GCS-beta2; Guanylate cyclase soluble beta 2 (GTP pyrophosphate - lyase); Guanylate cyclase soluble subunit beta-2; Guanylate cyclase, soluble, beta 2 (GTP pyrophosphate - lyase); soluble guanylate cyclase beta 2a; soluble guanylate cyclase beta 2b; soluble guanylyl cyclase beta2 subunit
Gene Aliases: B930025B03; GC-SB2; Gucy1b2; Gucy1b2a; Gucy1b2b; SGC
UniProt ID: (Rat) P22717
Entrez Gene ID: (Human) 2974, (Rat) 25206, (Mouse) 239134
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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