Proteintech

Galectin-3 Monoclonal Antibody (1C1B2)

Galectin-3 Antibody in Immunocytochemistry (ICC/IF)

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Galectin-3 Antibody (60207-1-IG) in ICC/IF

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Galectin-3 antibody (Product # 60207-1-Ig) (Clone: 1C1B2) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Galectin-3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Galectin-3 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

Galectin-3 Antibody in Western Blot (WB)

Galectin-3 Antibody in Flow Cytometry (Flow)

Product Image Gallery

Product Details

60207-1-IG

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

View 17 publications 17 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:2,000

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 12 publications 12 publications

Flow Cytometry (Flow)

Assay-dependent

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

1C1B2

Immunogen

Galectin-3 Fusion Protein Ag6753 (1-250 aa encoded by BC001120)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.83 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MADNFSLHD ALSGSGNPNP QGWPGAWGNQ PAGAGGYPGA SYPGAYPGQA PPGAYPGQAP PGAYPGAPGA YPGAPAPGVY PGPPSGPGAY PSSGQPSATG AYPATGPYGA PAGPLIVPYN LPLPGGVVPR MLITILGTVK PNANRIALDF QRGNDVAFHF NPRFNENNRR VIVCNTKLDN NWGREERQSV FPFESGKPFK IQVLVEPDHF KVAVNDAHLL QYNHRVKKLN EISKLGISGD IDLTSASYTM I (1-250 aa encoded by BC001120)

Target Information

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Transmission of NLRP3-IL-1β Signals in Cerebral Ischemia and Reperfusion Injury: from Microglia to Adjacent Neuron and Endothelial Cells via IL-1β/IL-1R1/TRAF6.

Molecular neurobiology

Pan J,Peng J,Li X,Wang H,Rong X,Peng Y

Mon May 01 00:00:00 UTC 2023

Involvement of Galectin-3 in neurocognitive impairment in obstructive sleep apnea via regulating inflammation and oxidative stress through NLRP3.

Sleep medicine

Zong D,Liu X,Shen C,Liu T,Ouyang R

Sun Jan 01 00:00:00 UTC 2023

Hepatocyte-derived MASP1-enriched small extracellular vesicles activate HSCs to promote liver fibrosis.

Hepatology (Baltimore, Md.)

Liu X,Tan S,Liu H,Jiang J,Wang X,Li L,Wu B

Sat Apr 01 00:00:00 UTC 2023

Hepatocyte-derived MASP1-enriched small extracellular vesicles activate HSCs to promote liver fibrosis.

Hepatology (Baltimore, Md.)

Liu X,Tan S,Liu H,Jiang J,Wang X,Li L,Wu B

Sat Apr 01 00:00:00 UTC 2023

CD248 Regulates Wnt Signaling in Pericytes to Promote Angiogenesis and Tumor Growth in Lung Cancer.

Cancer research

Hong CL,Yu IS,Pai CH,Chen JS,Hsieh MS,Wu HL,Lin SW,Huang HP

Mon Oct 17 00:00:00 UTC 2022

CD248 Regulates Wnt Signaling in Pericytes to Promote Angiogenesis and Tumor Growth in Lung Cancer.

Cancer research

Hong CL,Yu IS,Pai CH,Chen JS,Hsieh MS,Wu HL,Lin SW,Huang HP

Mon Oct 17 00:00:00 UTC 2022

CD248 Regulates Wnt Signaling in Pericytes to Promote Angiogenesis and Tumor Growth in Lung Cancer.

Cancer research

Hong CL,Yu IS,Pai CH,Chen JS,Hsieh MS,Wu HL,Lin SW,Huang HP

Mon Oct 17 00:00:00 UTC 2022

Galectin-3 Is a Natural Binding Ligand of MCAM (CD146, MUC18) in Melanoma Cells and Their Interaction Promotes Melanoma Progression.

Biomolecules

Pang Y,Maxwell E,Sindrewicz-Goral P,Shapanis A,Li S,Morgan M,Yu LG

Mon Oct 10 00:00:00 UTC 2022

Modified Citrus Pectin Alleviates Cerebral Ischemia/Reperfusion Injury by Inhibiting NLRP3 Inflammasome Activation via TLR4/NF-ĸB Signaling Pathway in Microglia.

Journal of inflammation research

Cui Y,Zhang NN,Wang D,Meng WH,Chen HS

Sat Jul 16 00:00:00 UTC 2022

Modified Citrus Pectin Alleviates Cerebral Ischemia/Reperfusion Injury by Inhibiting NLRP3 Inflammasome Activation via TLR4/NF-ĸB Signaling Pathway in Microglia.

Journal of inflammation research

Cui Y,Zhang NN,Wang D,Meng WH,Chen HS

Sat Jul 16 00:00:00 UTC 2022

Transcriptomic Analysis Reveals that Activating Transcription Factor 3/c-Jun/Lgals3 Axis Is Associated with Central Diabetes Insipidus after Hypothalamic Injury.

Neuroendocrinology

Zhou M,Ou Y,Wu G,Li K,Peng J,Wang X,Che M,Gong H,Niu P,Liu Y,Feng Z,Qi S

Tue Aug 23 00:00:00 UTC 2022

Single-cell RNA sequencing reveals a novel inhibitory effect of ApoA4 on NAFL mediated by liver-specific subsets of myeloid cells.

Frontiers in immunology

Liu XH,Zhou JT,Yan CX,Cheng C,Fan JN,Xu J,Zheng Q,Bai Q,Li Z,Li S,Li X

Mon Nov 28 00:00:00 UTC 2022

Thiamine pretreatment improves endotoxemia-related liver injury and cholestatic complications by regulating galactose metabolism and inhibiting macrophage activation.

International immunopharmacology

Li T,Bai J,Du Y,Tan P,Zheng T,Chen Y,Cheng Y,Cai T,Huang M,Fu W,Wen J

Fri Jul 01 00:00:00 UTC 2022

Thiamine pretreatment improves endotoxemia-related liver injury and cholestatic complications by regulating galactose metabolism and inhibiting macrophage activation.

International immunopharmacology

Li T,Bai J,Du Y,Tan P,Zheng T,Chen Y,Cheng Y,Cai T,Huang M,Fu W,Wen J

Fri Jul 01 00:00:00 UTC 2022

Asparagine endopeptidase cleaves synaptojanin 1 and triggers synaptic dysfunction in Parkinson's disease.

Neurobiology of disease

Zou L,Zhang X,Xiong M,Meng L,Tian Y,Pan L,Yuan X,Chen G,Wang Z,Bu L,Yao Z,Zhang Z,Ye K,Zhang Z

Thu Jul 01 00:00:00 UTC 2021

Pro64His (rs4644) Polymorphism Within Galectin-3 Is a Risk Factor of Differentiated Thyroid Carcinoma and Affects the Transcriptome of Thyrocytes Engineered via CRISPR/Cas9 System.

Thyroid : official journal of the American Thyroid Association

Corrado A,Aceto R,Silvestri R,Dell'Anno I,Ricci B,Miglietta S,Romei C,Giovannoni R,Poliseno L,Evangelista M,Vitiello M,Cipollini M,Garritano S,Giusti L,Zallocco L,Elisei R,Landi S,Gemignani F

Thu Jul 01 00:00:00 UTC 2021

Galectin‑3 facilitates the proliferation and migration of nasopharyngeal carcinoma cells via activation of the ERK1/2 and Akt signaling pathways, and is positively correlated with the inflammatory state of nasopharyngeal carcinoma.

Molecular medicine reports

Li M,Chen YB,Liu F,Qu JQ,Ren LC,Chai J,Tang CE

Sat May 01 00:00:00 UTC 2021

Molecular medicine reports

Li M,Chen YB,Liu F,Qu JQ,Ren LC,Chai J,Tang CE

Sat May 01 00:00:00 UTC 2021

Yin-xing-tong-mai decoction attenuates atherosclerosis via activating PPARγ-LXRα-ABCA1/ABCG1 pathway.

Pharmacological research

Zheng S,Huang H,Li Y,Wang Y,Zheng Y,Liang J,Zhang S,Liu M,Fang Z

Thu Jul 01 00:00:00 UTC 2021

Quantitative proteomics reveals the selectivity of ubiquitin-binding autophagy receptors in the turnover of damaged lysosomes by lysophagy.

eLife

Eapen VV,Swarup S,Hoyer MJ,Paulo JA,Harper JW

Wed Sep 29 00:00:00 UTC 2021

Quantitative proteomics reveals the selectivity of ubiquitin-binding autophagy receptors in the turnover of damaged lysosomes by lysophagy.

eLife

Eapen VV,Swarup S,Hoyer MJ,Paulo JA,Harper JW

Wed Sep 29 00:00:00 UTC 2021

Tumor cell membrane-based peptide delivery system targeting the tumor microenvironment for cancer immunotherapy and diagnosis.

Acta biomaterialia

Meng X,Wang J,Zhou J,Tian Q,Qie B,Zhou G,Duan W,Zhu Y

Tue Jun 01 00:00:00 UTC 2021

17β-Estradiol Attenuates LPS-Induced Macrophage Inflammation In Vitro and Sepsis-Induced Vascular Inflammation In Vivo by Upregulating miR-29a-5p Expression.

Mediators of inflammation

Zhang ML,Chen H,Yang Z,Zhang MN,Wang X,Zhao K,Li X,Xiu N,Tong F,Wang YX

Fri Jan 07 00:00:00 UTC 2022

Galectin-3 Contributes to the Inhibitory Effect of lα,25-(OH)₂D₃ on Osteoclastogenesis.

International journal of molecular sciences

Gu J,Zhang X,Zhang C,Li Y,Bian J,Liu X,Yuan Y,Zou H,Tong X,Liu Z

Sat Dec 11 00:00:00 UTC 2021

Galectin-3 Contributes to the Inhibitory Effect of lα,25-(OH)₂D₃ on Osteoclastogenesis.

International journal of molecular sciences

Gu J,Zhang X,Zhang C,Li Y,Bian J,Liu X,Yuan Y,Zou H,Tong X,Liu Z

Sat Dec 11 00:00:00 UTC 2021

Salvia miltiorrhiza-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis.

Theranostics

Yang GS,Zheng B,Qin Y,Zhou J,Yang Z,Zhang XH,Zhao HY,Yang HJ,Wen JK

Wed May 05 00:00:00 UTC 2021

Galectin-3 expression and secretion by tumor-associated macrophages in hypoxia promotes breast cancer progression.

Biochemical pharmacology

Wang L,Li YS,Yu LG,Zhang XK,Zhao L,Gong FL,Yang XX,Guo XL

Sat Aug 01 00:00:00 UTC 2020

Leukemia cells apoptosis by a newly discovered heterogeneous polysaccharide from Angelica sinensis (Oliv.) Diels.

Carbohydrate polymers

Liu W,Xiao K,Ren L,Sui Y,Chen J,Zhang T,Li XQ,Cao W

Sat Aug 01 00:00:00 UTC 2020

Fine particulate matter inhibits phagocytosis of macrophages by disturbing autophagy.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Li Y,Yong YL,Yang M,Wang W,Qu X,Dang X,Shang D,Shao Y,Liu J,Chang Y

Tue Dec 01 00:00:00 UTC 2020

Gasdermin D Drives the Nonexosomal Secretion of Galectin-3, an Insulin Signal Antagonist.

Journal of immunology (Baltimore, Md. : 1950)

Chen Y,Wang H,Shen J,Deng R,Yao X,Guo Q,Lu A,Sun B,Zhang Y,Meng G

Fri Nov 15 00:00:00 UTC 2019

Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm.

Circulation research

Ma D,Zheng B,Suzuki T,Zhang R,Jiang C,Bai D,Yin W,Yang Z,Zhang X,Hou L,Zhan H,Wen JK

Fri Mar 03 00:00:00 UTC 2017

Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm.

Circulation research

Ma D,Zheng B,Suzuki T,Zhang R,Jiang C,Bai D,Yin W,Yang Z,Zhang X,Hou L,Zhan H,Wen JK

Fri Mar 03 00:00:00 UTC 2017

Hypermethylation of IFN-γ in oral cancer tissues.

Clinical oral investigations

Tian S,Jiang C,Liu X,Xu S,Zhang Z,Chen H,Zhang Y,Liu Y,Ma D

Wed Nov 01 00:00:00 UTC 2017

Hypermethylation of IFN-γ in oral cancer tissues.

Clinical oral investigations

Tian S,Jiang C,Liu X,Xu S,Zhang Z,Chen H,Zhang Y,Liu Y,Ma D

Wed Nov 01 00:00:00 UTC 2017

Hepatocellular Carcinomas Originate Predominantly from Hepatocytes and Benign Lesions from Hepatic Progenitor Cells.

Cell reports

Tummala KS,Brandt M,Teijeiro A,Graña O,Schwabe RF,Perna C,Djouder N

Tue Apr 18 00:00:00 UTC 2017

Hepatocellular Carcinomas Originate Predominantly from Hepatocytes and Benign Lesions from Hepatic Progenitor Cells.

Cell reports

Tummala KS,Brandt M,Teijeiro A,Graña O,Schwabe RF,Perna C,Djouder N

Tue Apr 18 00:00:00 UTC 2017

Hepatocellular Carcinomas Originate Predominantly from Hepatocytes and Benign Lesions from Hepatic Progenitor Cells.

Cell reports

Tummala KS,Brandt M,Teijeiro A,Graña O,Schwabe RF,Perna C,Djouder N

Tue Apr 18 00:00:00 UTC 2017

Hepatocellular Carcinomas Originate Predominantly from Hepatocytes and Benign Lesions from Hepatic Progenitor Cells.

Cell reports

Tummala KS,Brandt M,Teijeiro A,Graña O,Schwabe RF,Perna C,Djouder N

Tue Apr 18 00:00:00 UTC 2017

Galectin-3 mediates pulmonary vascular remodeling in hypoxia-induced pulmonary arterial hypertension.

Journal of the American Society of Hypertension : JASH

Luo H,Liu B,Zhao L,He J,Li T,Zha L,Li X,Qi Q,Liu Y,Yu Z

Sun Oct 01 00:00:00 UTC 2017

Galectin-3 mediates pulmonary vascular remodeling in hypoxia-induced pulmonary arterial hypertension.

Journal of the American Society of Hypertension : JASH

Luo H,Liu B,Zhao L,He J,Li T,Zha L,Li X,Qi Q,Liu Y,Yu Z

Sun Oct 01 00:00:00 UTC 2017

Tongxinluo inhibits neointimal formation by regulating the expression and post-translational modification of KLF5 in macrophages.

American journal of translational research

Jiang W,Zheng B,Zhang XH,Yue LY,Liu C,Ma D,Yang Z,Wen JK

Wed Jan 13 00:00:00 UTC 2021

Tongxinluo inhibits neointimal formation by regulating the expression and post-translational modification of KLF5 in macrophages.

American journal of translational research

Jiang W,Zheng B,Zhang XH,Yue LY,Liu C,Ma D,Yang Z,Wen JK

Wed Jan 13 00:00:00 UTC 2021

miR-155-dependent regulation of mammalian sterile 20-like kinase 2 (MST2) coordinates inflammation, oxidative stress and proliferation in vascular smooth muscle cells.

Biochimica et biophysica acta

Yang Z,Zheng B,Zhang Y,He M,Zhang XH,Ma D,Zhang RN,Wu XL,Wen JK

Wed Jul 01 00:00:00 UTC 2015

miR-155-dependent regulation of mammalian sterile 20-like kinase 2 (MST2) coordinates inflammation, oxidative stress and proliferation in vascular smooth muscle cells.

Biochimica et biophysica acta

Yang Z,Zheng B,Zhang Y,He M,Zhang XH,Ma D,Zhang RN,Wu XL,Wen JK

Wed Jul 01 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: 35 kDa lectin; advanced glycation end-product receptor 3; Carbohydrate-binding protein 35; CBP 35; CBP-30; epsilon BP; Gal-3; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; Galectin-3; Galectin3; IgE binding protein; IgE-binding protein; L-29; L-31; L-34 galactoside-binding lectin; Laminin-binding protein; lectin galactoside-binding soluble 3 protein; Lectin L-29; lectin, galactose binding, soluble 3; lectin, galactoside-binding, soluble, 3; MAC-2 antigen

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Gene Aliases: AGE-R3; CBP30; CBP35; gal-3; GAL3; GALBP; GALIG; GBP; L-34; L31; LGALS2; LGALS3; Mac-2; MAC2

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UniProt ID: (Human) P17931, (Rat) P08699

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Entrez Gene ID: (Human) 3958, (Mouse) 16854, (Rat) 83781

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Function(s)

protein binding IgE binding carbohydrate binding chemoattractant activity laminin binding poly(A) RNA binding Fc-gamma receptor I complex binding monosaccharide binding disaccharide binding advanced glycation end-product receptor activity

Process(es)

monocyte chemotaxis mRNA processing RNA splicing neutrophil chemotaxis epithelial cell differentiation regulation of T cell proliferation innate immune response negative regulation of endocytosis eosinophil chemotaxis macrophage chemotaxis negative regulation of T cell receptor signaling pathway positive chemotaxis regulation of T cell apoptotic process mononuclear cell migration positive regulation of mononuclear cell migration positive regulation of calcium ion import regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of immunological synapse formation negative regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell positive regulation of dendritic cell differentiation negative regulation of extrinsic apoptotic signaling pathway skeletal system development immune system process signal transduction positive regulation of cell proliferation positive regulation of serotonin secretion cell differentiation extracellular matrix organization negative regulation of apoptotic process positive regulation of angiogenesis negative regulation of cell proliferation in bone marrow

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Galectin-3 Monoclonal Antibody (1C1B2)

Galectin-3 Antibody (60207-1-IG) in ICC/IF

Product Details

60207-1-IG

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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