Invitrogen

Galectin 3 Monoclonal Antibody (A3A12)

Galectin 3 Antibody in Immunocytochemistry (ICC/IF)

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Galectin 3 Antibody (MA1-940) in ICC/IF

Immunofluorescent analysis of Galectin 3 (green) showing staining in the cytoplasm and nucleus of NIH-3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Galectin 3 monoclonal antibody (Product # MA1-940) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC i... View More

Galectin 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Galectin 3 Antibody in Immunohistochemistry (IHC)

Galectin 3 Antibody in Western Blot (WB)

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Product Details

MA1-940

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 8 publications 8 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunohistochemistry (Frozen) (IHC (F))

6 µg/mL

Immunocytochemistry (ICC/IF)

1:20-1:200

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

A3A12

Immunogen

Full length recombinant human galectin-3.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2136775

Product Specific Information

MA1-940 detects galectin-3 from human, mouse and rat tissues.

MA1-940 has been successfully used in Western blot, immunohistochemistry, immunofluorescence, immunocytochemistry and ELISA procedures. By Western blot, this antibody detects an ~30 kDa protein representing galectin-3 from Jurkat cells transfected with human galectin-3. Immunohistochemical staining of galectin-3 in rat olfactory bulb with MA1-940 yields a pattern consistent with nuclear and plasma membrane staining. This antibody is not suitable for mouse NIH-3T3 in Western blot analysis. This antibody also detects a non-specific band in Hela cell lysates at ~21 kDa.

MA1-940 antigen is full length recombinant human galectin-3. The epitope for this antibody has been mapped to the first 58 amino acids of galectin-3.

Target Information

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Deregulation of mTORC1-TFEB axis in human iPSC model of GBA1-associated Parkinson's disease.

Frontiers in neuroscience

Mubariz F,Saadin A,Lingenfelter N,Sarkar C,Banerjee A,Lipinski MM,Awad O

MA1-940 was used in Western Blotting to investigate the effect of GBA1 mutations on the transcription factor EB (TFEB), the master regulator of the autophagy-lysosomal pathway (ALP).

Fri Jul 14 00:00:00 UTC 2023

Deregulation of mTORC1-TFEB axis in human iPSC model of GBA1-associated Parkinson's disease.

Frontiers in neuroscience

Mubariz F,Saadin A,Lingenfelter N,Sarkar C,Banerjee A,Lipinski MM,Awad O

MA1-940 was used in Western Blotting to investigate the effect of GBA1 mutations on the transcription factor EB (TFEB), the master regulator of the autophagy-lysosomal pathway (ALP).

Fri Jul 14 00:00:00 UTC 2023

Impairment of the ER/mitochondria compartment in human cardiomyocytes with PLN p.Arg14del mutation.

EMBO molecular medicine

Cuello F,Knaust AE,Saleem U,Loos M,Raabe J,Mosqueira D,Laufer S,Schweizer M,van der Kraak P,Flenner F,Ulmer BM,Braren I,Yin X,Theofilatos K,Ruiz-Orera J,Patone G,Klampe B,Schulze T,Piasecki A,Pinto Y,Vink A,Hübner N,Harding S,Mayr M,Denning C,Eschenhagen T,Hansen A

MA1-940 was used in Western Blot to identify impairment of the ER/mitochondria compartment without SR dysfunction as a novel disease mechanism underlying PLN p.Arg14del cardiomyopathy.

Mon Jun 07 00:00:00 UTC 2021

Impairment of the ER/mitochondria compartment in human cardiomyocytes with PLN p.Arg14del mutation.

EMBO molecular medicine

MA1-940 was used in Western Blot to identify impairment of the ER/mitochondria compartment without SR dysfunction as a novel disease mechanism underlying PLN p.Arg14del cardiomyopathy.

Mon Jun 07 00:00:00 UTC 2021

Stimulation of β-adrenoceptors up-regulates cardiac expression of galectin-3 and BIM through the Hippo signalling pathway.

British journal of pharmacology

Zhao WB,Lu Q,Nguyen MN,Su Y,Ziemann M,Wang LN,Kiriazis H,Puthalakath H,Sadoshima J,Hu HY,Du XJ

MA1-940 was used in Western Blotting to study the mechanism by which B-adrenoceptors regulate the expression of pro-fibrotic galectin-3 and apoptotic BIM.

Mon Jul 01 00:00:00 UTC 2019

Stimulation of β-adrenoceptors up-regulates cardiac expression of galectin-3 and BIM through the Hippo signalling pathway.

British journal of pharmacology

Zhao WB,Lu Q,Nguyen MN,Su Y,Ziemann M,Wang LN,Kiriazis H,Puthalakath H,Sadoshima J,Hu HY,Du XJ

MA1-940 was used in Western Blotting to study the mechanism by which B-adrenoceptors regulate the expression of pro-fibrotic galectin-3 and apoptotic BIM.

Mon Jul 01 00:00:00 UTC 2019

Immunogold labelling in environmental scanning electron microscopy: applicative features for complementary cytological interpretation.

Journal of microscopy

Cafiero G,Papale F,Grimaldi A,Rosso F,Barbarisi M,Tortora C,Marino G,Barbarisi A

MA1-940 was used in immunohistochemistry to evaluate a new environmental scanning electron microscopy techniques with immunogold labelling for the study of cell morphology

Sat Jan 01 00:00:00 UTC 2011

A novel phenylpyridazinone, T-3999, reduces the progression of autoimmune myocarditis to dilated cardiomyopathy.

Heart and vessels

Kamal FA,Watanabe K,Ma M,Abe Y,Elbarbary R,Kodama M,Aizawa Y

MA1-940 was used in western blot to investigate the therapeutic effectiveness of phenylpyridazinone T-3999 in autoimmune myocarditis

Sat Jan 01 00:00:00 UTC 2011

Biofilm-like extracellular viral assemblies mediate HTLV-1 cell-to-cell transmission at virological synapses.

Nature medicine

Pais-Correia AM,Sachse M,Guadagnini S,Robbiati V,Lasserre R,Gessain A,Gout O,Alcover A,Thoulouze MI

MA1-940 was used in immunocytochemistry to study the mechanism of HTLV-1 cell-to-cell transmission at virological synapses

Fri Jan 01 00:00:00 UTC 2010

Partial inactivation of cardiac 14-3-3 protein in vivo elicits endoplasmic reticulum stress (ERS) and activates ERS-initiated apoptosis in ERS-induced mice.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Sari FR,Watanabe K,Widyantoro B,Thandavarayan RA,Harima M,Zhang S,Muslin AJ,Kodama M,Aizawa Y

MA1-940 was used in western blot to investigate the role of 14-3-3 protein in endoplasmic reticulum stress and apoptosis

Wed Dec 08 00:00:00 UTC 2010

Galectin-3 reduces the severity of pneumococcal pneumonia by augmenting neutrophil function.

The American journal of pathology

Farnworth SL,Henderson NC,Mackinnon AC,Atkinson KM,Wilkinson T,Dhaliwal K,Hayashi K,Simpson AJ,Rossi AG,Haslett C,Sethi T

MA1-940 was used in western blot to find galectin-3(-/-) mice infected with Streptococcus pneumoniae develop severe pneumonia

Fri Feb 01 00:00:00 UTC 2008

Up-regulation of advanced glycated products receptors in the brain of diabetic rats is prevented by antioxidant treatment.

Endocrinology

Aragno M,Mastrocola R,Medana C,Restivo F,Catalano MG,Pons N,Danni O,Boccuzzi G

MA1-940 was used in western blot to investigate the effect of antioxidant on advanced glycated products receptors in the brain.

Thu Dec 01 00:00:00 UTC 2005

Galectin-3-positive cell infiltration in human diabetic nephropathy.

Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association

Kikuchi Y,Kobayashi S,Hemmi N,Ikee R,Hyodo N,Saigusa T,Namikoshi T,Yamada M,Suzuki S,Miura S

MA1-940 was used in immunohistochemistry to investigate the role of galectin-3-positive cell infiltration in the progression of human diabetic nephropathy

Mon Mar 01 00:00:00 UTC 2004

Galectin-3 messenger ribonucleic acid and protein are expressed in benign thyroid tumors.

The Journal of clinical endocrinology and metabolism

Martins L,Matsuo SE,Ebina KN,Kulcsar MA,Friguglietti CU,Kimura ET

MA1-940 was used in immunohistochemistry to investigate galectin-3 expression in benign thyroid tumors.

Tue Oct 01 00:00:00 UTC 2002

Expression of galectin-3 modulates T-cell growth and apoptosis.

Proceedings of the National Academy of Sciences of the United States of America

Yang RY,Hsu DK,Liu FT

MA1-940 was used in western blot to investigate the impacts of galectin-3 on the growth and apoptosis of T cells

Tue Jun 25 00:00:00 UTC 1996

Bioinformatics

Protein Aliases: 35 kDa lectin; advanced glycation end-product receptor 3; Carbohydrate-binding protein 35; CBP 35; CBP-30; epsilon BP; Gal-3; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; Galectin-3; Galectin3; IgE binding protein; IgE-binding protein; L-29; L-31; L-34 galactoside-binding lectin; Laminin-binding protein; lectin galactoside-binding soluble 3 protein; Lectin L-29; lectin, galactose binding, soluble 3; lectin, galactoside-binding, soluble, 3; MAC-2 antigen

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Gene Aliases: AGE-R3; CBP30; CBP35; gal-3; GAL3; GALBP; GALIG; GBP; L-34; L31; LGALS2; LGALS3; Mac-2; MAC2

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UniProt ID: (Human) P17931, (Rat) P08699

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Entrez Gene ID: (Human) 3958, (Mouse) 16854, (Rat) 83781

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Function(s)

protein binding IgE binding carbohydrate binding chemoattractant activity laminin binding poly(A) RNA binding Fc-gamma receptor I complex binding monosaccharide binding disaccharide binding advanced glycation end-product receptor activity

Process(es)

monocyte chemotaxis mRNA processing RNA splicing neutrophil chemotaxis epithelial cell differentiation regulation of T cell proliferation innate immune response negative regulation of endocytosis eosinophil chemotaxis macrophage chemotaxis negative regulation of T cell receptor signaling pathway positive chemotaxis regulation of T cell apoptotic process mononuclear cell migration positive regulation of mononuclear cell migration positive regulation of calcium ion import regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of immunological synapse formation negative regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell positive regulation of dendritic cell differentiation negative regulation of extrinsic apoptotic signaling pathway skeletal system development immune system process signal transduction positive regulation of cell proliferation positive regulation of serotonin secretion cell differentiation extracellular matrix organization negative regulation of apoptotic process positive regulation of angiogenesis negative regulation of cell proliferation in bone marrow

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Galectin 3 Monoclonal Antibody (A3A12)

Galectin 3 Antibody (MA1-940) in ICC/IF

Product Details

MA1-940

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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