Invitrogen

Galectin 3 Monoclonal Antibody (eBioM3/38 (M3/38)), PE, eBioscience™

Galectin 3 Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 5

Galectin 3 Antibody (12-5301-81) in Flow

Staining of C57BL/6 bone marrow cells with Anti-Mouse CD11b FITC (Product # 11-0112-41) and Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (left) or 0.03 µg of Anti-Human/Mouse Galectin-3 PE (right). Total viable cells were used for analysis.

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Product Details

12-5301-81

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.06 µg/test

View 4 publications 4 publications

Product Specifications

Published species

Hamster, Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioM3/38 (M3/38)

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

AB_842794

Product Specific Information

Description: The eBioM3/38 antibody reacts with mouse Galectin-3 (Mac-2). Galectins are a family of animal lectins which appear to exhibit a variety of biological functions. Galectin 3 (aka LGALS3, galactose-specific soluble lectin 3, Mac-2, L-29) is one of the more extensively studied members of this family and is a 30 kDa beta-galactoside-binding protein. Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homo-oligomerized. Galectin 3 is normally distributed in epithelia of many organs and various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins.

This monoclonal antibody eBioM3/38 crossreacts to human Galectin-3 (LGALS3).

Applications Reported: This eBioM3/38 (M3/38) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioM3/38 (M3/38) antibody has been tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal macrophages. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Development of a Syrian hamster anti-PD-L1 monoclonal antibody enables oncolytic adenoviral immunotherapy modelling in an immunocompetent virus replication permissive setting.

Frontiers in immunology

Clubb JHA,Kudling TV,Girych M,Haybout L,Pakola S,Hamdan F,Cervera-Carrascon V,Hemmes A,Grönberg-Vähä-Koskela S,Santos JM,Quixabeira DCA,Basnet S,Heiniö C,Arias V,Jirovec E,Kaptan S,Havunen R,Sorsa S,Erikat A,Schwartz J,Anttila M,Aro K,Viitala T,Vattulainen I,Cerullo V,Kanerva A,Hemminki A

12-5301-82 was used in Flow cytometry/Cell sorting to develop an anti-Syrian hamster PD-L1 mAb to enable the evaluation of safety and efficacy, when combining anti-PD-L1 with an oncolytic adenovirus encoding tumour necrosis factor alpha (TNFα) and interleukin-2 (IL-2) (Ad5/3-E2F-D24-hTNFα-IRES-hIL-2 or TILT-123).

Mon Feb 27 00:00:00 UTC 2023

Endocytic membrane repair by ESCRT-III controls antigen export to the cytosol during antigen cross-presentation.

Cell reports

Gros M,Segura E,Rookhuizen DC,Baudon B,Heurtebise-Chrétien S,Burgdorf N,Maurin M,Kapp EA,Simpson RJ,Kozik P,Villadangos JA,Bertrand MJM,Burbage M,Amigorena S

12-5301-82 was used in Immunohistochemistry-immunofluorescence to demonstrate that membrane repair proves crucial in containing antigen export to the cytosol and cross-presentation in cDCs.

Tue Aug 16 00:00:00 UTC 2022

Cell surface galectin-3 defines a subset of chemoresistant gastrointestinal tumor-initiating cancer cells with heightened stem cell characteristics.

Cell death & disease

Ilmer M,Mazurek N,Byrd JC,Ramirez K,Hafley M,Alt E,Vykoukal J,Bresalier RS

12-5301 was used in Flow cytometry/Cell sorting to examine the ability of Gal3 to characterise the cancer stem cell (CSC) phenotype, and identify a clinically important gastrointestinal cancer CSC population.

Thu Aug 11 00:00:00 UTC 2016

Maternal immune activation evoked by polyinosinic:polycytidylic acid does not evoke microglial cell activation in the embryo.

Frontiers in cellular neuroscience

Smolders S,Smolders SM,Swinnen N,Gärtner A,Rigo JM,Legendre P,Brône B

12-5301 was used in Flow cytometry/Cell sorting to indicate that activation of the fetal microglial cells is not likely to be responsible for the inflammation induced deficits in this model for neurodevelopmental disorders.

Mon Aug 24 00:00:00 UTC 2015

Galectin-9 controls the therapeutic activity of 4-1BB-targeting antibodies.

The Journal of experimental medicine

Madireddi S,Eun SY,Lee SW,Nemčovičová I,Mehta AK,Zajonc DM,Nishi N,Niki T,Hirashima M,Croft M

12-5301 was used in Flow cytometry/Cell sorting to delineate the mechanism of action of agonist anti-4-1BB in suppressing autoimmune and allergic inflammation.

Mon Jun 30 00:00:00 UTC 2014

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