Hamburger Menu Button
Thermo Fisher Scientific Logo
Sign in
Don't have an account ? Create Account
  • Products
    • Antibodies
    • Cell Culture Media
    • Chemicals
    • Chromatography Columns and Cartridges
    • Lab Equipment
    • Lab Plasticware and Supplies
    • Microplates
    • Oligos, Primers, Probes and Genes
    • TaqMan Real-Time PCR Assays
    • Greener Products
    • See all product categories
  • Applications
    • Bioprocessing
    • Cell Culture and Transfection
    • Cell and Gene Therapy
    • Chromatography
    • Molecular Testing
    • Digital Solutions
    • DNA and RNA Extraction and Analysis
    • Spectroscopy, Elemental and Isotope Analysis
    • See all applications and techniques
  • Services
    • 360° CDMO and CRO Solutions
    • CDMO Services
    • CRO Services
    • Custom Services
    • Enterprise Services
    • Financial and Leasing Services
    • Instrument Services
    • Lab Informatics
    • OEM and Commercial Supply
    • Training Services
    • Unity Lab Services
    • See all services
  • Help and Support
    • How to Order
    • Instrument Support
    • Learning Centers
    • Register for an Account
    • Technical Support Centers
    • See all help and support topics
  • Popular
    • TaqMan Real-Time PCR Assays
      TaqMan Real-Time PCR Assays
    • Antibodies
      Antibodies
    • Oligos, Primers & Probes
      Oligos, Primers & Probes
    • GeneArt Gene Synthesis
      GeneArt Gene Synthesis
    • Cell Culture Plastics
      Cell Culture Plastics
  • Contact Us
  • Quick Order
  • Order Status and Tracking
  • Documents and Certificates
Thermo Fisher Scientific Logo

Search Thermo Fisher Scientific

Search All
Search button Close
          • Order Status
          • Quick Order
          • Sign in
            Sign in
            Don't have an account ? Create Account
            • Account
            • Check Order Status
            • Aspire Member Program
            • Connect: Lab, Data, Apps
            • Custom Products & Projects
            • Services Central

          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Galectin 3 Antibodies

          Invitrogen

          Galectin 3 Monoclonal Antibody (eBioM3/38 (M3/38)), eBioscience™

          Advanced Verification
          36 Published Figures
          48 References
          View all (131) Galectin 3 antibodies

          Promotion

          View available promotion(s)

          Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

          Datasheet
          Protocols
          Questions & Answers
          Datasheet
          Protocols
          Questions & Answers

          Cite Galectin 3 Monoclonal Antibody (eBioM3/38 (M3/38)), eBioscience™

          • Antibody Testing Data (5)
          • Published Figures (36)
          • Advanced Verification (2)
          Galectin 3 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Galectin 3 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 43

          {{ $ctrl.currentElement.advancedVerification.fullName }}
          {{ $ctrl.currentElement.advancedVerification.text }}

          Galectin 3 Antibody (14-5301-82) in ICC/IF

          Immunofluorescence analysis of Galectin 3 was performed using 70% confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Galectin 3 Monoclonal Antibody (eBioM3/38 (M3/38)), eBioscience™ (Product # 14-5301-85) at 1:100 dilut... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          View Product

          {{$ctrl.videoDesc}}

          {{$ctrl.videoLongDesc}}

          Galectin 3 Antibody in Immunocytochemistry (ICC/IF)
          Galectin 3 Antibody in Western Blot (WB)
          Galectin 3 Antibody in Western Blot (WB)
          Galectin 3 Antibody in Western Blot (WB)
          Galectin 3 Antibody in Flow Cytometry (Flow)
          Galectin 3 Antibody in Immunocytochemistry (ICC/IF)
          Galectin 3 Antibody in Immunocytochemistry (ICC/IF)
          Galectin 3 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          Galectin 3 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)
          Galectin 3 Antibody in Immunohistochemistry, Immunohistochemistry (Paraffin) (IHC, IHC (P))
          Galectin 3 Antibody in Immunohistochemistry (IHC)
          Galectin 3 Antibody in Immunohistochemistry (IHC)
          Galectin 3 Antibody in Immunohistochemistry (IHC)
          Galectin 3 Antibody in Immunohistochemistry (IHC)
          Galectin 3 Antibody in Immunohistochemistry (IHC)
          Galectin 3 Antibody in Immunohistochemistry (IHC)
          Galectin 3 Antibody in Immunohistochemistry (IHC)
          Galectin 3 Antibody in Immunohistochemistry (IHC)
          Galectin 3 Antibody in Immunohistochemistry (IHC)
          Galectin 3 Antibody in Immunohistochemistry (IHC)
          Galectin 3 Antibody in Immunohistochemistry (IHC)
          Galectin 3 Antibody in Immunohistochemistry (IHC)
          Galectin 3 Antibody in Western Blot, Immunohistochemistry (WB, IHC)
          Galectin 3 Antibody in Immunohistochemistry (IHC)
          Galectin 3 Antibody in Immunohistochemistry (IHC)
          Galectin 3 Antibody in Immunohistochemistry (IHC)
          Galectin 3 Antibody in Immunohistochemistry (IHC)
          Galectin 3 Antibody in Immunohistochemistry (IHC)
          Galectin 3 Antibody in Immunohistochemistry (IHC)
          Galectin 3 Antibody in Immunohistochemistry (IHC)
          Galectin 3 Antibody in Immunohistochemistry (IHC)
          Galectin 3 Antibody in Immunohistochemistry (IHC)
          Galectin 3 Antibody in Immunohistochemistry (IHC)
          Galectin 3 Antibody in Western Blot (WB)
          Galectin 3 Antibody in Western Blot (WB)
          Galectin 3 Antibody in Western Blot (WB)
          Galectin 3 Antibody in Western Blot (WB)
          Galectin 3 Antibody in Western Blot, Flow Cytometry (WB, Flow)
          Galectin 3 Antibody in Flow Cytometry (Flow)
          Galectin 3 Antibody in Flow Cytometry (Flow)
          Galectin 3 Antibody in Flow Cytometry (Flow)
          Galectin 3 Antibody
          Galectin 3 Antibody

          Product Details

          14-5301-82

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.5 µg/mL
          View 7 publications 7 publications

          Immunohistochemistry (IHC)

          -
          View 30 publications 30 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          Assay-Dependent
          View 3 publications 3 publications

          Immunohistochemistry (Frozen) (IHC (F))

          Assay-Dependent
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          1:100
          View 4 publications 4 publications

          Flow Cytometry (Flow)

          0.25 µg/test
          View 3 publications 3 publications

          ELISA (ELISA)

          Assay-Dependent
          -

          Immunoprecipitation (IP)

          Assay-Dependent
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Rat / IgG2a, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          eBioM3/38 (M3/38)

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • Biotin 
          • eFluor 660
          • PE 
          • Request custom conjugation

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.2

          Contains

          0.09% sodium azide

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_837132

          Product Specific Information

          Description: The eBioM3/38 antibody reacts with mouse Galectin-3 (Mac-2). Galectins are a family of animal lectins which appear to exhibit a variety of biological functions. Galectin 3 (aka LGALS3, galactose-specific soluble lectin 3, Mac-2, L-29) is one of the more extensively studied members of this family and is a 30 kDa beta-galactoside-binding protein. Due to a C-terminal carbohydrate binding site, Galectin 3 is capable of binding IgE and mammalian cell surfaces only when homodimerized or homo-oligomerized. Galectin 3 is normally distributed in epithelia of many organs and various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins.

          This monoclonal antibody eBioM3/38 crossreacts to human Galectin-3 (LGALS3).

          Applications Reported: This eBioM3/38 (M3/38) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), immunohistology staining of frozen tissue sections, immunohistology staining of paraffin embedded tissue sections, and ELISA.

          Applications Tested: This eBioM3/38 (M3/38) antibody has been tested by flow cytometric analysis of mouse PECs (thioglycollated elicied peritioneal cells). This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Purity: Greater than 90%, as determined by SDS-PAGE.

          Aggregation: Less than 10%, as determined by HPLC.

          Filtration: 0.2 µm post-manufacturing filtered.

          Target Information

          Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: 35 kDa lectin; advanced glycation end-product receptor 3; Carbohydrate-binding protein 35; CBP 35; CBP-30; Gal-3; Galactose-specific lectin 3; Galactoside-binding protein; GALBP; Galectin-3; Galectin3; IgE-binding protein; L-29; L-31; L-34 galactoside-binding lectin; Laminin-binding protein; Lectin L-29; lectin, galactoside-binding, soluble, 3; MAC-2 antigen

          View more View less

          Gene Aliases: CBP35; GAL3; GALBP; GALIG; GBP; L-34; L31; LGALS2; LGALS3; Mac-2; MAC2

          View more View less

          UniProt ID: (Human) P17931

          View more View less

          Entrez Gene ID: (Human) 3958, (Mouse) 16854

          View more View less

          Function(s)
          protein binding IgE binding carbohydrate binding chemoattractant activity laminin binding poly(A) RNA binding Fc-gamma receptor I complex binding monosaccharide binding disaccharide binding advanced glycation end-product receptor activity
          Process(es)
          monocyte chemotaxis mRNA processing RNA splicing neutrophil chemotaxis epithelial cell differentiation regulation of T cell proliferation innate immune response negative regulation of endocytosis eosinophil chemotaxis macrophage chemotaxis negative regulation of T cell receptor signaling pathway positive chemotaxis regulation of T cell apoptotic process mononuclear cell migration positive regulation of mononuclear cell migration positive regulation of calcium ion import regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of immunological synapse formation negative regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell positive regulation of dendritic cell differentiation negative regulation of extrinsic apoptotic signaling pathway skeletal system development immune system process signal transduction positive regulation of cell proliferation positive regulation of serotonin secretion cell differentiation extracellular matrix organization negative regulation of apoptotic process positive regulation of angiogenesis negative regulation of cell proliferation in bone marrow
          It has to be done as per old AB suggested Products section.
          guarantee_icon

          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
          help_icon

          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

          Contact tech support
          Ordering Plus Icon Minus Icon
          • Order Status
          • Order Help
          • Quick Order
          • Supply Center
          • eProcurement
          Support Plus Icon Minus Icon
          • Help and Support
          • Contact Us
          • Technical Support Centers
          • Documents and Certificates
          • Report a Site Issue
          Resources Plus Icon Minus Icon
          • Learning Centers
          • Promotions
          • Events and Webinars
          • Social Media
          About Thermo Fisher Plus Icon Minus Icon
          • About Us
          • Careers
          • Investors
          • News
          • Social Responsibility
          • Trademarks
          • Consumer Health Data Privacy Policy
          Our Portfolio Plus Icon Minus Icon
          • Thermo Scientific
          • Applied Biosystems
          • Invitrogen
          • Gibco
          • Ion Torrent
          • Fisher Scientific
          • Unity Lab Services
          • Patheon
          • PPD
          • Terms & Conditions
          • Privacy Information Center
          • Price & Freight Policy
          © 2006-2025 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
          United States flag icon
          United States

          Your items have has been added!


          Host server : magellan-search-67c77f6dc7-n6nf2:80/100.66.135.186:80.
          git-commit: 545e792480003146e539d624bd2a21b51d198228
          git-url: https://github.com/thermofisher/magellan-search
          git-branch: release/2.31.0-2025.06.23-1.0