Invitrogen

Gata-3 Monoclonal Antibody (TWAJ), eBioscience™

Gata-3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 31

Gata-3 Antibody (14-9966-82) in ICC/IF

Knockdown of GATA-3 was achieved by transfecting MCF7 cells with GATA-3 specific siRNAs (Silencer® select Product # s5600; s5599). Immunofluorescence analysis was performed using untransfected MCF7 (panels a, d), transfected with non-specific scrambled siRNA (panels b,e) and transfected with GATA-3 specific siRNAs (panel c,f). The cells were labeled with GATA-3 Monoclonal Antibody (TWAJ) (Product # 14-9966-80) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight, followed by labelling with Goat anti-Rat IgG (H+L) Superclonal™ Se... View More

Gata-3 Antibody in Immunohistochemistry (IHC)

Gata-3 Antibody in Immunohistochemistry, Immunohistochemistry (PFA fixed) (IHC, IHC (PFA))

Gata-3 Antibody in Flow Cytometry (Flow)

Product Image Gallery

Product Details

14-9966-82

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

5 µg/mL

View 3 publications 3 publications

Flow Cytometry (Flow)

View 47 publications 47 publications

Miscellaneous PubMed (MISC)

View 3 publications 3 publications

Mass Spectrometry (MS)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Pig, Rhesus monkey

Published species

Bat, Human, Mouse

Host/Isotype

Rat / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

TWAJ

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1210519

Product Specific Information

Description: The monoclonal antibody TWAJ recognizes mouse and human Gata-3, a member of the Gata family of transcription factors. Gata-3 is a T cell-specific transcription factor important for thymic development and Th2 differentiation. Expression during embryonic development is found in the central nervous system, skin, mammary glands and kidney. During development, the expression of Gata-3 is essential as homozygous knock-out of Gata-3 is embryonic lethal. The Gata-3 is also essential for T cell commitment and survival. In the thymus, expression is found mainly on the CD4 single positive cells. During Th2 differentiation, Gata-3 binds to the IL-4 promoter as well as represses the expression of T-bet, thus inhibiting Th1 differentiation.

Alternative splice variants have been reported especially in the MCF7 cell line. The TWAJ Human/Mouse Gata-3 antibody will recognize both forms (50 and 45 kDa) of the protein.

Staining with the TWAJ Human/Mouse Gata-3 antibody requires the use of the Foxp3/Transcription Factor Staining Buffer Set

Target Information

The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Th2 cells inhibit growth of colon and pancreas cancers by promoting anti-tumorigenic responses from macrophages and eosinophils.

British journal of cancer

Jacenik D,Karagiannidis I,Beswick EJ

Published figure using Gata-3 monoclonal antibody (Product # 14-9966-82) in Immunohistochemistry

Sun Jan 01 00:00:00 UTC 2023

Airway Administration of Bacterial Lysate OM-85 Protects Mice Against Respiratory Syncytial Virus Infection.

Frontiers in immunology

Antunes KH,Cassão G,Santos LD,Borges SG,Poppe J,Gonçalves JB,Nunes EDS,Recacho GF,Sousa VB,Da Silva GS,Mansur D,Stein RT,Pasquali C,De Souza APD

Published figure using Gata-3 monoclonal antibody (Product # 14-9966-82) in Flow Cytometry

Tue May 24 00:00:00 UTC 2022

GM-CSF-activated human dendritic cells promote type 1 T follicular helper cell polarization in a CD40-dependent manner.

Journal of cell science

Korniotis S,Saichi M,Trichot C,Hoffmann C,Amblard E,Viguier A,Grondin S,Noel F,Mattoo H,Soumelis V

Published figure using Gata-3 monoclonal antibody (Product # 14-9966-82) in Flow Cytometry

Tue Nov 01 00:00:00 UTC 2022

The allergy mediator histamine confers resistance to immunotherapy in cancer patients via activation of the macrophage histamine receptor H1.

Cancer cell

Li H,Xiao Y,Li Q,Yao J,Yuan X,Zhang Y,Yin X,Saito Y,Fan H,Li P,Kuo WL,Halpin A,Gibbons DL,Yagita H,Zhao Z,Pang D,Ren G,Yee C,Lee JJ,Yu D

14-9966-82 was used in Immunocytochemistry to uncover that histamine and histamine receptor H1 (HRH1) are frequently increased in the tumor microenvironment and induce T cell dysfunction.

Mon Jan 10 00:00:00 UTC 2022

Stromal Reprogramming by FAK Inhibition Overcomes Radiation Resistance to Allow for Immune Priming and Response to Checkpoint Blockade.

Cancer discovery

Lander VE,Belle JI,Kingston NL,Herndon JM,Hogg GD,Liu X,Kang LI,Knolhoff BL,Bogner SJ,Baer JM,Zuo C,Borcherding NC,Lander DP,Mpoy C,Scott J,Zahner M,Rogers BE,Schwarz JK,Kim H,DeNardo DG

14-9966-82 was used in Mass Spectrometry to show that FAK inhibition allows RT to prime tumor immunity and unlock responsiveness to checkpoint immunotherapy.

Fri Dec 02 00:00:00 UTC 2022

Modeling human extraembryonic mesoderm cells using naive pluripotent stem cells.

Cell stem cell

Pham TXA,Panda A,Kagawa H,To SK,Ertekin C,Georgolopoulos G,van Knippenberg SSFA,Allsop RN,Bruneau A,Chui JS,Vanheer L,Janiszewski A,Chappell J,Oberhuemer M,Tchinda RS,Talon I,Khodeer S,Rossant J,Lluis F,David L,Rivron N,Balaton BP,Pasque V

14-9966-82 was used in Immunocytochemistry to investigate the mechanisms involved in differentiation of naive human pluripotent stem cells into EXM cells.

Thu Sep 01 00:00:00 UTC 2022

The molecular and epigenetic mechanisms of innate lymphoid cell (ILC) memory and its relevance for asthma.

The Journal of experimental medicine

Verma M,Michalec L,Sripada A,McKay J,Sirohi K,Verma D,Sheth D,Martin R,Dyjack N,Seibold MA,Knapp JR,Tu TH,O'Connor BP,Gorska MM,Alam R

Published figure using Gata-3 monoclonal antibody (Product # 14-9966-82) in Flow Cytometry

Mon Jul 05 00:00:00 UTC 2021

CD8⁺ T cells inhibit metastasis and CXCL4 regulates its function.

British journal of cancer

Joseph R,Soundararajan R,Vasaikar S,Yang F,Allton KL,Tian L,den Hollander P,Isgandarova S,Haemmerle M,Mino B,Zhou T,Shin C,Martinez-Paniagua M,Sahin AA,Rodriguez-Canales J,Gelovani J,Chang JT,Acharya G,Sood AK,Wistuba II,Gibbons DL,Solis LM,Barton MC,Varadarajan N,Rosen JM,Zhang XH,Mani SA

14-9966-82 was used in Mass cytometry to reveal that intratumoral immune infiltration differs between metastatic and non-metastatic tumours.

Thu Jul 01 00:00:00 UTC 2021

Effector T cell responses unleashed by regulatory T cell ablation exacerbate oral squamous cell carcinoma.

Cell reports. Medicine

Chao JL,Korzinkin M,Zhavoronkov A,Ozerov IV,Walker MT,Higgins K,Lingen MW,Izumchenko E,Savage PA

Published figure using Gata-3 monoclonal antibody (Product # 14-9966-82) in Flow Cytometry

Tue Sep 21 00:00:00 UTC 2021

Graded RhoA GTPase Expression in Treg Cells Distinguishes Tumor Immunity From Autoimmunity.

Frontiers in immunology

Kalim KW,Yang JQ,Modur V,Nguyen P,Li Y,Zheng Y,Guo F

Published figure using Gata-3 monoclonal antibody (Product # 14-9966-82) in Flow Cytometry

Tue Jan 11 00:00:00 UTC 2022

A coordinated progression of progenitor cell states initiates urinary tract development.

Nature communications

Sanchez-Ferras O,Pacis A,Sotiropoulou M,Zhang Y,Wang YC,Bourgey M,Bourque G,Ragoussis J,Bouchard M

14-9966-80 was used in Immunocytochemistry to provide a better understanding of the cellular origin of the renal collecting duct system and associated urinary tract developmental diseases, which may inform guided differentiation of functional kidney tissue.

Tue May 11 00:00:00 UTC 2021

A coordinated progression of progenitor cell states initiates urinary tract development.

Nature communications

Sanchez-Ferras O,Pacis A,Sotiropoulou M,Zhang Y,Wang YC,Bourgey M,Bourque G,Ragoussis J,Bouchard M

Tue May 11 00:00:00 UTC 2021

A coordinated progression of progenitor cell states initiates urinary tract development.

Nature communications

Sanchez-Ferras O,Pacis A,Sotiropoulou M,Zhang Y,Wang YC,Bourgey M,Bourque G,Ragoussis J,Bouchard M

Tue May 11 00:00:00 UTC 2021

LmjF.36.3850, a novel hypothetical Leishmania major protein, contributes to the infection.

Immunology

Zutshi S,Sarode AY,Ghosh SK,Jha MK,Sudan R,Kumar S,Sadhale LP,Roy S,Saha B

Published figure using Gata-3 monoclonal antibody (Product # 14-9966-82) in Flow Cytometry

Sun Aug 01 00:00:00 UTC 2021

Histone deacetylase 3 represses cholesterol efflux during CD4⁺ T-cell activation.

eLife

Wilfahrt D,Philips RL,Lama J,Kizerwetter M,Shapiro MJ,McCue SA,Kennedy MM,Rajcula MJ,Zeng H,Shapiro VS

Published figure using Gata-3 monoclonal antibody (Product # 14-9966-82) in Flow Cytometry

Thu Dec 02 00:00:00 UTC 2021

Microbial exposure during early human development primes fetal immune cells.

Cell

Mishra A,Lai GC,Yao LJ,Aung TT,Shental N,Rotter-Maskowitz A,Shepherdson E,Singh GSN,Pai R,Shanti A,Wong RMM,Lee A,Khyriem C,Dutertre CA,Chakarov S,Srinivasan KG,Shadan NB,Zhang XM,Khalilnezhad S,Cottier F,Tan ASM,Low G,Chen P,Fan Y,Hor PX,Lee AKM,Choolani M,Vermijlen D,Sharma A,Fuks G,Straussman R,Pavelka N,Malleret B,McGovern N,Albani S,Chan JKY,Ginhoux F

14-9966-82 was used in Immunohistochemistry, Immunocytochemistry, Western Blot to indicate selective presence of live microbes in fetal organs during the 2nd trimester of gestation and have broader implications toward the establishment of immune competency and priming before birth.

Thu Jun 24 00:00:00 UTC 2021

Cell Types Promoting Goosebumps Form a Niche to Regulate Hair Follicle Stem Cells.

Cell

Shwartz Y,Gonzalez-Celeiro M,Chen CL,Pasolli HA,Sheu SH,Fan SM,Shamsi F,Assaad S,Lin ET,Zhang B,Tsai PC,He M,Tseng YH,Lin SJ,Hsu YC

14-9966-82 was used in Immunohistochemistry to demonstrate sympathetic nerves can modulate stem cells through synapse-like connections and neurotransmitters to couple tissue production with demands.

Thu Aug 06 00:00:00 UTC 2020

Attenuation of TCR-induced transcription by Bach2 controls regulatory T cell differentiation and homeostasis.

Nature communications

Sidwell T,Liao Y,Garnham AL,Vasanthakumar A,Gloury R,Blume J,Teh PP,Chisanga D,Thelemann C,de Labastida Rivera F,Engwerda CR,Corcoran L,Kometani K,Kurosaki T,Smyth GK,Shi W,Kallies A

Published figure using Gata-3 monoclonal antibody (Product # 14-9966-82) in Flow Cytometry

Tue Jan 14 00:00:00 UTC 2020

Bendamustine with total body irradiation conditioning yields tolerant T-cells while preserving T-cell-dependent graft-versus-leukemia.

Oncoimmunology

Stokes J,Hoffman EA,Molina MS,Kummet N,Simpson RJ,Zeng Y,Katsanis E

Published figure using Gata-3 monoclonal antibody (Product # 14-9966-82) in Flow Cytometry

Wed Jul 14 00:00:00 UTC 2021

Intestinal vitamin D receptor knockout protects from oxazolone-induced colitis.

Cell death & disease

Shi Y,Liu Z,Cui X,Zhao Q,Liu T

14-9966 was used in Western Blotting to identify that intestinal vitamin D receptor knockout protected against oxazolone-induced colitis in mice by blocking Th2 cell response and reducing the function of intestinal invariant natural killer T cells.

Mon Jun 15 00:00:00 UTC 2020

In Situ Characterization of Follicular Helper CD4 T Cells Using Multiplexed Imaging.

Frontiers in immunology

Ioannidou K,Ndiaye DR,Noto A,Fenwick C,Fortis SP,Pantaleo G,Petrovas C,de Leval L

14-9966-82 was used in Mass Spectrometry to provide the base for future studies aiming to a comprehensive understanding of Tfh cell tissue heterogeneity.

Wed Jun 23 00:00:00 UTC 2021

The obesity-induced adipokine sST2 exacerbates adipose T_reg and ILC2 depletion and promotes insulin resistance.

Science advances

Zhao XY,Zhou L,Chen Z,Ji Y,Peng X,Qi L,Li S,Lin JD

14-9966 was used in Flow cytometry/Cell sorting to identify sST2 as an obesity-induced adipokine that contributes to regulatory T cell and group 2 innate lymphoid cell dysregulation and insulin resistance in mice.

Fri May 01 00:00:00 UTC 2020

The obesity-induced adipokine sST2 exacerbates adipose T_reg and ILC2 depletion and promotes insulin resistance.

Science advances

Zhao XY,Zhou L,Chen Z,Ji Y,Peng X,Qi L,Li S,Lin JD

Fri May 01 00:00:00 UTC 2020

Compartmentalized gut lymph node drainage dictates adaptive immune responses.

Nature

Esterházy D,Canesso MCC,Mesin L,Muller PA,de Castro TBR,Lockhart A,ElJalby M,Faria AMC,Mucida D

Published figure using Gata-3 monoclonal antibody (Product # 14-9966-82) in Flow Cytometry

Wed May 01 00:00:00 UTC 2019

IL-33-mediated mast cell activation promotes gastric cancer through macrophage mobilization.

Nature communications

Eissmann MF,Dijkstra C,Jarnicki A,Phesse T,Brunnberg J,Poh AR,Etemadi N,Tsantikos E,Thiem S,Huntington ND,Hibbs ML,Boussioutas A,Grimbaldeston MA,Buchert M,O'Donoghue RJJ,Masson F,Ernst M

Published figure using Gata-3 monoclonal antibody (Product # 14-9966-82) in Flow Cytometry

Fri Jun 21 00:00:00 UTC 2019

Perturbed myoepithelial cell differentiation in BRCA mutation carriers and in ductal carcinoma in situ.

Nature communications

Ding L,Su Y,Fassl A,Hinohara K,Qiu X,Harper NW,Huh SJ,Bloushtain-Qimron N,Jovanović B,Ekram M,Zi X,Hines WC,Alečković M,Gil Del Alcazar C,Caulfield RJ,Bonal DM,Nguyen QD,Merino VF,Choudhury S,Ethington G,Panos L,Grant M,Herlihy W,Au A,Rosson GD,Argani P,Richardson AL,Dillon D,Allred DC,Babski K,Kim EMH,McDonnell CH,Wagner J,Rowberry R,Bobolis K,Kleer CG,Hwang ES,Blum JL,Cristea S,Sicinski P,Fan R,Long HW,Sukumar S,Park SY,Garber JE,Bissell M,Yao J,Polyak K

14-9966-82 was used in Mass cytometry to investigate myoepithelial cells in normal breast tissues of BRCA1 and BRCA2 germline mutation carriers and in non-carrier controls, and in sporadic DCIS.

Fri Sep 13 00:00:00 UTC 2019

Tissue-Restricted Adaptive Type 2 Immunity Is Orchestrated by Expression of the Costimulatory Molecule OX40L on Group 2 Innate Lymphoid Cells.

Immunity

Halim TYF,Rana BMJ,Walker JA,Kerscher B,Knolle MD,Jolin HE,Serrao EM,Haim-Vilmovsky L,Teichmann SA,Rodewald HR,Botto M,Vyse TJ,Fallon PG,Li Z,Withers DR,McKenzie ANJ

Published figure using Gata-3 monoclonal antibody (Product # 14-9966-82) in Flow Cytometry

Tue Jun 19 00:00:00 UTC 2018

Oxysterol Sensing through the Receptor GPR183 Promotes the Lymphoid-Tissue-Inducing Function of Innate Lymphoid Cells and Colonic Inflammation.

Immunity

Emgård J,Kammoun H,García-Cassani B,Chesné J,Parigi SM,Jacob JM,Cheng HW,Evren E,Das S,Czarnewski P,Sleiers N,Melo-Gonzalez F,Kvedaraite E,Svensson M,Scandella E,Hepworth MR,Huber S,Ludewig B,Peduto L,Villablanca EJ,Veiga-Fernandes H,Pereira JP,Flavell RA,Willinger T

14-9966 was used in Immunocytochemistry-immunofluorescence to show that GPR183 promotes lymphoid organ development and indicate that oxysterol-GPR183-dependent positioning within tissues controls ILC3 activity and intestinal homeostasis.

Tue Jan 16 00:00:00 UTC 2018

Fas ligand promotes an inducible TLR-dependent model of cutaneous lupus-like inflammation.

The Journal of clinical investigation

Mande P,Zirak B,Ko WC,Taravati K,Bride KL,Brodeur TY,Deng A,Dresser K,Jiang Z,Ettinger R,Fitzgerald KA,Rosenblum MD,Harris JE,Marshak-Rothstein A

14-9966 was used in Flow cytometry/Cell sorting to provide a relevant system for exploring the pathophysiology of cutaneous lupus erythematosus (CLE) as well as the negative regulatory role of TLR9.

Mon Jul 02 00:00:00 UTC 2018

Mass cytometry reveals innate lymphoid cell differentiation pathways in the human fetal intestine.

The Journal of experimental medicine

Li N,van Unen V,Höllt T,Thompson A,van Bergen J,Pezzotti N,Eisemann E,Vilanova A,Chuva de Sousa Lopes SM,Lelieveldt BPF,Koning F

14-9966-82 was used in Flow Cytometry to identify a previously unknown intermediate innate subset that can differentiate into ILC3 and NK cells.

Mon May 07 00:00:00 UTC 2018

CD4⁺ T Cell Fate Decisions Are Stochastic, Precede Cell Division, Depend on GITR Co-Stimulation, and Are Associated With Uropodium Development.

Frontiers in immunology

Cobbold SP,Adams E,Howie D,Waldmann H

Published figure using Gata-3 monoclonal antibody (Product # 14-9966-82) in Flow Cytometry

Mon Jan 31 00:00:00 UTC 2022

IgE promotes type 2 innate lymphoid cells in murine food allergy.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology

Burton OT,Medina Tamayo J,Stranks AJ,Miller S,Koleoglou KJ,Weinberg EO,Oettgen HC

14-9966 was used in Flow cytometry/Cell sorting to determine the effect of IgE mediated mast cell activation on intestinal ILC3 responses following ingestion of food allergens.

Thu Mar 01 00:00:00 UTC 2018

Stabilization of cytokine mRNAs in iNKT cells requires the serine-threonine kinase IRE1alpha.

Nature communications

Govindarajan S,Gaublomme D,Van der Cruyssen R,Verheugen E,Van Gassen S,Saeys Y,Tavernier S,Iwawaki T,Bloch Y,Savvides SN,Lambrecht BN,Janssens S,Elewaut D,Drennan MB

14-9966 was used in Flow cytometry/Cell sorting to examine the role of the IRE1α-dependent signalling cascade in coupling constitutive cytokine mRNA expression to the rapid induction of cytokine secretion and effector functions in activated iNKT cells.

Mon Dec 17 00:00:00 UTC 2018

Flt3 ligand expands bona fide innate lymphoid cell precursors in vivo.

Scientific reports

Parigi SM,Czarnewski P,Das S,Steeg C,Brockmann L,Fernandez-Gaitero S,Yman V,Forkel M,Höög C,Mjösberg J,Westerberg L,Färnert A,Huber S,Jacobs T,Villablanca EJ

14-9966 was used in Flow cytometry/Cell sorting to indicate that Flt3L expands CHILPs in the bone marrow, which might be associated with specific inflammatory conditions.

Tue Jan 09 00:00:00 UTC 2018

Stability and function of regulatory T cells expressing the transcription factor T-bet.

Nature

Levine AG,Mendoza A,Hemmers S,Moltedo B,Niec RE,Schizas M,Hoyos BE,Putintseva EV,Chaudhry A,Dikiy S,Fujisawa S,Chudakov DM,Treuting PM,Rudensky AY

Published figure using Gata-3 monoclonal antibody (Product # 14-9966-82) in Flow Cytometry

Thu Jun 15 00:00:00 UTC 2017

Dissection of SAP-dependent and SAP-independent SLAM family signaling in NKT cell development and humoral immunity.

The Journal of experimental medicine

Chen S,Cai C,Li Z,Liu G,Wang Y,Blonska M,Li D,Du J,Lin X,Yang M,Dong Z

14-9966-82 was used in Flow Cytometry to examine NKT cell development and humoral immunity in mice completely deficient in SFR.

Wed Feb 01 00:00:00 UTC 2017

PD-1 regulates KLRG1⁺ group 2 innate lymphoid cells.

The Journal of experimental medicine

Taylor S,Huang Y,Mallett G,Stathopoulou C,Felizardo TC,Sun MA,Martin EL,Zhu N,Woodward EL,Elias MS,Scott J,Reynolds NJ,Paul WE,Fowler DH,Amarnath S

14-9966-82 was used in Flow Cytometry to report that PD-1 is required for maintaining the number, and hence function, of KLRG1+ Group 2 innate lymphoid cells.

Mon Jun 05 00:00:00 UTC 2017

NLRP3 signaling drives macrophage-induced adaptive immune suppression in pancreatic carcinoma.

The Journal of experimental medicine

Daley D,Mani VR,Mohan N,Akkad N,Pandian GSDB,Savadkar S,Lee KB,Torres-Hernandez A,Aykut B,Diskin B,Wang W,Farooq MS,Mahmud AI,Werba G,Morales EJ,Lall S,Wadowski BJ,Rubin AG,Berman ME,Narayanan R,Hundeyin M,Miller G

14-9966-82 was used in Flow Cytometry to suggest that targeting NLRP3 holds the promise for the immunotherapy of pancreatic ductal adenocarcinoma.

Mon Jun 05 00:00:00 UTC 2017

NLRP3 signaling drives macrophage-induced adaptive immune suppression in pancreatic carcinoma.

The Journal of experimental medicine

14-9966-82 was used in Flow Cytometry to suggest that targeting NLRP3 holds the promise for the immunotherapy of pancreatic ductal adenocarcinoma.

Mon Jun 05 00:00:00 UTC 2017

Protein kinase CK2 controls T-cell polarization through dendritic cell activation in response to contact sensitizers.

Journal of leukocyte biology

de Bourayne M,Gallais Y,El Ali Z,Rousseau P,Damiens MH,Cochet C,Filhol O,Chollet-Martin S,Pallardy M,Kerdine-Römer S

Published figure using Gata-3 monoclonal antibody (Product # 14-9966-82) in Flow Cytometry

Wed Mar 01 00:00:00 UTC 2017

NK cells regulate CXCR2+ neutrophil recruitment during acute lung injury.

Journal of leukocyte biology

Hoegl S,Ehrentraut H,Brodsky KS,Victorino F,Golden-Mason L,Eltzschig HK,McNamee EN

14996682 was used in flow cytometry to elucidate the role of Tbet in acute lung injury

Wed Feb 01 00:00:00 UTC 2017

Characterisation of innate lymphoid cell populations at different sites in mice with defective T cell immunity.

Wellcome open research

Dutton EE,Camelo A,Sleeman M,Herbst R,Carlesso G,Belz GT,Withers DR

Published figure using Gata-3 monoclonal antibody (Product # 14-9966-82) in Flow Cytometry

Thu Mar 14 00:00:00 UTC 2024

An asthma-associated IL4R variant exacerbates airway inflammation by promoting conversion of regulatory T cells to TH17-like cells.

Nature medicine

Massoud AH,Charbonnier LM,Lopez D,Pellegrini M,Phipatanakul W,Chatila TA

14-9966 was used in Flow cytometry/Cell sorting to identify a previously unknown mechanism for the development of TH17 cell inflammation in genetically prone individuals.

Thu Sep 01 00:00:00 UTC 2016

FOXP3+ Tregs require WASP to restrain Th2-mediated food allergy.

The Journal of clinical investigation

Lexmond WS,Goettel JA,Lyons JJ,Jacobse J,Deken MM,Lawrence MG,DiMaggio TH,Kotlarz D,Garabedian E,Sackstein P,Nelson CC,Jones N,Stone KD,Candotti F,Rings EH,Thrasher AJ,Milner JD,Snapper SB,Fiebiger E

14-9966 was used in Flow cytometry/Cell sorting to investigate the mechanism by which FOXP3+ T regulatory cells and WASP protein together restrain Th-2 mediated food allergy.

Mon Oct 03 00:00:00 UTC 2016

Group 2 innate lymphoid cells utilize the IRF4-IL-9 module to coordinate epithelial cell maintenance of lung homeostasis.

Mucosal immunology

Mohapatra A,Van Dyken SJ,Schneider C,Nussbaum JC,Liang HE,Locksley RM

14-9966 was used in Flow cytometry/Cell sorting to study how ILC2s link alveolar function to regulation of airway flow, revealing a key interaction between resident lymphoid and structural cells that might underlie similar organisational hierarchies in other organs.

Fri Jan 01 00:00:00 UTC 2016

Monocyte-Derived Dendritic Cells Promote Th Polarization, whereas Conventional Dendritic Cells Promote Th Proliferation.

Journal of immunology (Baltimore, Md. : 1950)

Chow KV,Lew AM,Sutherland RM,Zhan Y

14-9966 was used in Flow cytometry/Cell sorting to investigate how division of labor between moDCs and cDCs has implications for their respective roles in the immune response.

Fri Jan 15 00:00:00 UTC 2016

Ndfip-mediated degradation of Jak1 tunes cytokine signalling to limit expansion of CD4+ effector T cells.

Nature communications

O'Leary CE,Riling CR,Spruce LA,Ding H,Kumar S,Deng G,Liu Y,Seeholzer SH,Oliver PM

14996682 was used in flow cytometry to demonstrate that Ndfip1/Ndfip2 regulate cross talk between T-cell receptor and cytokine signaling pathways

Mon Apr 18 00:00:00 UTC 2016

Phenotypic and functional characterization of the major lymphocyte populations in the fruit-eating bat Pteropus alecto.

Scientific reports

Martínez Gómez JM,Periasamy P,Dutertre CA,Irving AT,Ng JH,Crameri G,Baker ML,Ginhoux F,Wang LF,Alonso S

14996682 was used in flow cytometry to describe the phenotypic and functional characterization of T cell subsets, B and NK cells in the fruit-eating bat Pteropus alecto

Thu Nov 24 00:00:00 UTC 2016

Conditions for the generation of cytotoxic CD4(+) Th cells that enhance CD8(+) CTL-mediated tumor regression.

Clinical & translational immunology

Li K,Baird M,Yang J,Jackson C,Ronchese F,Young S

14-9966 was used in Flow cytometry/Cell sorting to provide valuable data for further research into in vitro expansion of CD4(+) Th1-like cells, with potential applications to cancer treatment involving ACT.

Mon Aug 01 00:00:00 UTC 2016

Retinoic Acid Differentially Regulates the Migration of Innate Lymphoid Cell Subsets to the Gut.

Immunity

Kim MH,Taparowsky EJ,Kim CH

14996682 was used in flow cytometry to discuss tissue tropisms of innate lymphoid cells

Tue Jul 21 00:00:00 UTC 2015

Intranasal Administration of Lentiviral miR-135a Regulates Mast Cell and Allergen-Induced Inflammation by Targeting GATA-3.

PloS one

Deng YQ,Yang YQ,Wang SB,Li F,Liu MZ,Hua QQ,Tao ZZ

Published figure using Gata-3 monoclonal antibody (Product # 14-9966-82) in Flow Cytometry

Tue May 31 00:00:00 UTC 2016

Chronic Trichuris muris Infection in C57BL/6 Mice Causes Significant Changes in Host Microbiota and Metabolome: Effects Reversed by Pathogen Clearance.

PloS one

Houlden A,Hayes KS,Bancroft AJ,Worthington JJ,Wang P,Grencis RK,Roberts IS

14-9966 was used in Flow cytometry/Cell sorting to investigate the effects of T. muris infection on the faecal microbiota of mice.

Wed Apr 06 00:00:00 UTC 2016

The AGC kinase SGK1 regulates TH1 and TH2 differentiation downstream of the mTORC2 complex.

Nature immunology

Heikamp EB,Patel CH,Collins S,Waickman A,Oh MH,Sun IH,Illei P,Sharma A,Naray-Fejes-Toth A,Fejes-Toth G,Misra-Sen J,Horton MR,Powell JD

14-9966 was used in Western Blotting to characterise the range of phenotypes generated for mice with selective deletion of SGK1 in T cells.

Thu May 01 00:00:00 UTC 2014

HTLV-1 induces a Th1-like state in CD4+CCR4+ T cells.

The Journal of clinical investigation

Araya N,Sato T,Ando H,Tomaru U,Yoshida M,Coler-Reilly A,Yagishita N,Yamauchi J,Hasegawa A,Kannagi M,Hasegawa Y,Takahashi K,Kunitomo Y,Tanaka Y,Nakajima T,Nishioka K,Utsunomiya A,Jacobson S,Yamano Y

14-9966 was used in Flow cytometry/Cell sorting to study the cellular changes leading to the development of an inflammatory response to Human T-lymphotropic virus type 1 infection.

Fri Aug 01 00:00:00 UTC 2014

Differential requirement for Nfil3 during NK cell development.

Journal of immunology (Baltimore, Md. : 1950)

Seillet C,Huntington ND,Gangatirkar P,Axelsson E,Minnich M,Brady HJ,Busslinger M,Smyth MJ,Belz GT,Carotta S

14-9966 was used in Flow cytometry/Cell sorting to study how Nfil3 drives the formation of mature NK cells by inducing Eomes expression and reveal the differential requirements of NK cell subsets for Nfil3.

Sat Mar 15 00:00:00 UTC 2014

Cutting edge: Failure of antigen-specific CD4+ T cell recruitment to the kidney during systemic candidiasis.

Journal of immunology (Baltimore, Md. : 1950)

Drummond RA,Wallace C,Reid DM,Way SS,Kaplan DH,Brown GD

14-9966 was used in Flow cytometry/Cell sorting to explain the redundancy of CD4(+) T cells for defense against systemic infection with Candida albicans by characterising C. albicans-specific T cells.

Mon Dec 01 00:00:00 UTC 2014

GATA3 suppresses metastasis and modulates the tumour microenvironment by regulating microRNA-29b expression.

Nature cell biology

Chou J,Lin JH,Brenot A,Kim JW,Provot S,Werb Z

Published figure using Gata-3 monoclonal antibody (Product # 14-9966-82) in Immunohistochemistry

Fri Feb 01 00:00:00 UTC 2013

Impaired proteasome function activates GATA3 in T cells and upregulates CTLA-4: relevance for Sézary syndrome.

The Journal of investigative dermatology

Gibson HM,Mishra A,Chan DV,Hake TS,Porcu P,Wong HK

Published figure using Gata-3 monoclonal antibody (Product # 14-9966-82) in Flow Cytometry

Tue Jan 01 00:00:00 UTC 2013

Nitric oxide-induced regulatory T cells inhibit Th17 but not Th1 cell differentiation and function.

Journal of immunology (Baltimore, Md. : 1950)

Niedbala W,Besnard AG,Jiang HR,Alves-Filho JC,Fukada SY,Nascimento D,Mitani A,Pushparaj P,Alqahtani MH,Liew FY

14-9966 was used in Flow cytometry/Cell sorting to demonstrate that CD4+CD25+Foxp3- regulatory T-cells suppress Th17 but not Th1 cell function and differentiation.

Mon Jul 01 00:00:00 UTC 2013

Mesenchymal stem cells alleviate TNBS-induced colitis by modulating inflammatory and autoimmune responses.

World journal of gastroenterology

Chen QQ,Yan L,Wang CZ,Wang WH,Shi H,Su BB,Zeng QH,Du HT,Wan J

Wed Aug 07 00:00:00 UTC 2013

Transcutaneous vaccination via laser microporation.

Journal of controlled release : official journal of the Controlled Release Society

Weiss R,Hessenberger M,Kitzmüller S,Bach D,Weinberger EE,Krautgartner WD,Hauser-Kronberger C,Malissen B,Boehler C,Kalia YN,Thalhamer J,Scheiblhofer S

14-9966 was used in Flow cytometry/Cell sorting to investigate the potential of transcutaneous immunisation via laser-generated micropores for induction of specific immune responses.

Mon Sep 10 00:00:00 UTC 2012

Bcl6 controls the Th2 inflammatory activity of regulatory T cells by repressing Gata3 function.

Journal of immunology (Baltimore, Md. : 1950)

Sawant DV,Sehra S,Nguyen ET,Jadhav R,Englert K,Shinnakasu R,Hangoc G,Broxmeyer HE,Nakayama T,Perumal NB,Kaplan MH,Dent AL

14-9966 was used in Flow cytometry/Cell sorting to investigate the role of Bcl6 in Treg function.

Thu Nov 15 00:00:00 UTC 2012

Micro-RNA dysregulation in multiple sclerosis favours pro-inflammatory T-cell-mediated autoimmunity.

Brain : a journal of neurology

Guerau-de-Arellano M,Smith KM,Godlewski J,Liu Y,Winger R,Lawler SE,Whitacre CC,Racke MK,Lovett-Racke AE

14-9966 was used in Flow cytometry/Cell sorting to illustrate the biological significance and therapeutic potential of these micro-RNAs in regulating T-cell phenotypes in multiple sclerosis.

Thu Dec 01 00:00:00 UTC 2011

Micro-RNA dysregulation in multiple sclerosis favours pro-inflammatory T-cell-mediated autoimmunity.

Brain : a journal of neurology

Guerau-de-Arellano M,Smith KM,Godlewski J,Liu Y,Winger R,Lawler SE,Whitacre CC,Racke MK,Lovett-Racke AE

14-9966 was used in Flow cytometry/Cell sorting to illustrate the biological significance and therapeutic potential of these micro-RNAs in regulating T-cell phenotypes in multiple sclerosis.

Thu Dec 01 00:00:00 UTC 2011

TLR4 signaling via MyD88 and TRIF differentially shape the CD4+ T cell response to Porphyromonas gingivalis hemagglutinin B.

Journal of immunology (Baltimore, Md. : 1950)

Gaddis DE,Michalek SM,Katz J

14-9966 was used in Flow cytometry/Cell sorting to investigate how the stimulation of dendritic cells with recombinant haemagglutinin B affects the activation and differentiation of T cells in mice.

Sun May 15 00:00:00 UTC 2011

Inflammation driven by tumour-specific Th1 cells protects against B-cell cancer.

Nature communications

Haabeth OA,Lorvik KB,Hammarström C,Donaldson IM,Haraldsen G,Bogen B,Corthay A

14-9966 was used in Flow cytometry/Cell sorting to investigate the nature of protective anti-tumour immunity.

Mon Aug 01 00:00:00 UTC 2011

Role of CD44 in the differentiation of Th1 and Th2 cells: CD44-deficiency enhances the development of Th2 effectors in response to sheep RBC and chicken ovalbumin.

Journal of immunology (Baltimore, Md. : 1950)

Guan H,Nagarkatti PS,Nagarkatti M

Wed Jul 01 00:00:00 UTC 2009

Bioinformatics

Protein Aliases: GATA binding factor-3; GATA binding protein 3; GATA binding protein-3; GATA-binding factor 3; GATA-binding protein 3; MGC2346; MGC5199; MGC5445; Trans-acting T-cell-specific transcription factor GATA-3; Transacting T-cell-specific transcription factor GATA-3

View more View less

Gene Aliases: EGK_19445; Gata-3; GATA3; HDR; HDRS; jal

View more View less

UniProt ID: (Human) P23771, (Mouse) P23772

View more View less

Entrez Gene ID: (Pig) 733631, (Rhesus monkey) 713840, (Human) 2625, (Mouse) 14462

View more View less

Function(s)

transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding core promoter sequence-specific DNA binding nucleic acid binding transcription factor activity transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity interleukin-2 receptor binding protein binding transcription factor binding zinc ion binding transcription regulatory region DNA binding protein dimerization activity E-box binding HMG box domain binding sequence-specific DNA binding metal ion binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

Gata-3 Monoclonal Antibody (TWAJ), eBioscience™

Gata-3 Antibody (14-9966-82) in ICC/IF

Product Details

14-9966-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (MISC)

Mass Spectrometry (MS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!