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          • Primary Antibodies ›
          • GATA3 Antibodies
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          Invitrogen

          Gata-3 Monoclonal Antibody (TWAJ), eBioscience™

          Advanced Verification
          26 Published Figures
          63 References
          View all (115) GATA3 antibodies

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          Datasheet
          Protocols
          Datasheet
          Protocols

          Cite Gata-3 Monoclonal Antibody (TWAJ), eBioscience™

          • Antibody Testing Data (3)
          • Published Figures (26)
          • Advanced Verification (2)
          Gata-3 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Gata-3 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 31

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          Gata-3 Antibody (14-9966-95) in ICC/IF

          Knockdown of GATA-3 was achieved by transfecting MCF7 cells with GATA-3 specific siRNAs (Silencer® select Product # s5600; s5599). Immunofluorescence analysis was performed using untransfected MCF7 (panels a, d), transfected with non-specific scrambled siRNA (panels b,e) and transfected with GATA-3 specific siRNAs (panel c,f). The cells were labeled with GATA-3 Monoclonal Antibody (TWAJ) (Product # 14-9966-80) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight, followed by labelling with Goat anti-Rat IgG (H+L) Superclonal™ Se... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          Gata-3 Antibody in Immunocytochemistry (ICC/IF)
          Gata-3 Antibody in Immunocytochemistry (ICC/IF)
          Gata-3 Antibody in Western Blot (WB)
          Gata-3 Antibody in Immunohistochemistry (IHC)
          Gata-3 Antibody in Immunohistochemistry, Immunohistochemistry (PFA fixed) (IHC, IHC (PFA))
          Gata-3 Antibody in Immunohistochemistry (IHC)
          Gata-3 Antibody in Western Blot (WB)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody in Flow Cytometry (Flow)
          Gata-3 Antibody
          Gata-3 Antibody

          Product Details

          14-9966-95

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          2 µg/mL
          View 3 publications 3 publications

          Immunohistochemistry (IHC)

          -
          View 5 publications 5 publications

          Immunohistochemistry (PFA fixed) (IHC (PFA))

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          5 µg/mL
          View 3 publications 3 publications

          Flow Cytometry (Flow)

          -
          View 47 publications 47 publications

          Miscellaneous PubMed (MISC)

          -
          View 3 publications 3 publications

          Mass Spectrometry (MS)

          -
          View 1 publication 1 publication
          Product Specifications

          Published species

          Bat, Human, Mouse

          Host/Isotype

          Rat / IgG2b, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          TWAJ

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • Alexa Fluor 488 
          • Alexa Fluor 700 
          • Brilliant Violet 605
          • eFluor 450
          • eFluor 660
          • PE 
          • PE-Cyanine5
          • PE-Cyanine7
          • PE-eFluor 610
          • PerCP-eFluor 710
          • Request custom conjugation

          Form

          Liquid

          Concentration

          0.5 mg/mL

          Storage conditions

          4°C

          Shipping conditions

          Wet ice

          RRID

          AB_2865542

          Product Specific Information

          Description: The monoclonal antibody TWAJ recognizes mouse and human Gata-3, a member of the Gata family of transcription factors. Gata-3 is a T cell-specific transcription factor important for thymic development and Th2 differentiation. Expression during embryonic development is found in the central nervous system, skin, mammary glands and kidney. During development, the expression of Gata-3 is essential as homozygous knock-out of Gata-3 is embryonic lethal. The Gata-3 is also essential for T cell commitment and survival. In the thymus, expression is found mainly on the CD4 single positive cells. During Th2 differentiation, Gata-3 binds to the IL-4 promoter as well as represses the expression of T-bet, thus inhibiting Th1 differentiation.

          Alternative splice variants have been reported especially in the MCF7 cell line. The TWAJ Human/Mouse Gata-3 antibody will recognize both forms (50 and 45 kDa) of the protein.

          Staining with the TWAJ Human/Mouse Gata-3 antibody requires the use of the Foxp3/Transcription Factor Staining Buffer Set

          Target Information

          The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          It has to be done as per old AB suggested Products section.
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