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Gata1 Antibody (600-401-K12) in WB
Western Blot of Rabbit Anti-Gata1 antibody. Lane 1: NIH/3T3. Lane 2: mouse brain. Load: 25 µg per lane. Primary antibody: Gata1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 60 min at RT. Block: MB-070 Fluorescent Blocking Buffer used overnight at 4°C. Predicted/Observed size: 42.7 kDa for Gata1.
Product Details
600-401-K12
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-Gata1 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to mouse Gata1 peptide.
Conjugate
Form
Liquid
Concentration
0.73 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Gata1 is directed against mouse Gata1. A BLAST analysis was used to suggest reactivity with this protein from mouse and rat based on 100% homology for the immunogen sequence and 93% to human. Cross reactivity with Gata1 homologues from other sources has not been determined.
Target Information
Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Eryf1; Erythroid transcription factor; GATA-1; GATA-binding factor 1; GATA-binding protein 1; HGNC:4170; NF-E1 DNA-binding protein
Gene Aliases: eryf1; Gata-1; Gata1; Gf-1
UniProt ID: (Mouse) P17679
Entrez Gene ID: (Mouse) 14460
transcription regulatory region sequence-specific DNA binding
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II transcription factor binding
enhancer sequence-specific DNA binding
p53 binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
zinc ion binding
DNA binding, bending
chromatin DNA binding
sequence-specific DNA binding
metal ion binding
C2H2 zinc finger domain binding
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
cell-cell signaling
negative regulation of cell proliferation
male gonad development
regulation of glycoprotein biosynthetic process
regulation of definitive erythrocyte differentiation
myeloid cell differentiation
erythrocyte differentiation
megakaryocyte differentiation
platelet formation
basophil differentiation
eosinophil differentiation
negative regulation of bone mineralization
positive regulation of osteoblast proliferation
embryonic hemopoiesis
eosinophil fate commitment
negative regulation of apoptotic process
positive regulation of erythrocyte differentiation
positive regulation of transcription from RNA polymerase II promoter
cell development
erythrocyte development
positive regulation of peptidyl-tyrosine phosphorylation
platelet aggregation
transcriptional activation by promoter-enhancer looping
dendritic cell differentiation
cellular response to thyroid hormone stimulus
negative regulation of transcription regulatory region DNA binding
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
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