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GbetaL Antibody (A300-679A) in IP
Detection of human GbetaL by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GbetaL antibody (Product # A300-679A) (lot 2) used for IP at 6 µg per reaction. GbetaL was also immunoprecipitated by rabbit anti-GbetaL antibody A300-680A. For blotting immunoprecipitated GbetaL, A300-680A was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
Product Details
A300-679A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residues 275 and the C-terminus (residue 326) of human G protein Beta subunit-like.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse, Rat and Bovine
Target Information
G-protein beta subunit like (GBL protein) is a novel member of WD-40 protein family. It is a highly conserved cytosolic protein consisting of seven WD-40 repeating motifs. LST8 is a functional component of mTOR signaling complex (TORC1) and positively up-regulates the mTOR pathway. It interacts with the kinase domain of mTOR and stabilizes its interaction with raptor. LST8 participates in regulating cell growth and also in nutrient and growth factor-mediated mTOR signaling to S6K1. Reports suggest up-regulated expression of LST8 due to insulin indicating a possible role of LST8 in intracellular events related to insulin-receptor activation including insulin-stimulated glucose transport and glycogen synthesis. Northern Blot analysis detected a ubiquitous expression of LST8 with highest expression in brain and testis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: G protein beta subunit-like; Gable; GBL protein; Mammalian lethal with SEC13 protein 8; mLST8; mLST8 / mLST8; protein GbetaL; Target of rapamycin complex subunit LST8; TORC subunit gable; TORC subunit LST8; TORC subunit WAT1
Gene Aliases: GbetaL; GBL; LST8; MLST8; POP3; WAT1
UniProt ID: (Human) Q9BVC4
Entrez Gene ID: (Human) 64223
cell cycle arrest
macroautophagy
positive regulation of actin filament polymerization
T cell costimulation
positive regulation of TOR signaling
regulation of actin cytoskeleton organization
regulation of GTPase activity
phosphatidylinositol-mediated signaling
positive regulation of peptidyl-tyrosine phosphorylation
regulation of cellular response to heat
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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