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Invitrogen
Gemin 2 Monoclonal Antibody (2E17)
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FIGURE: 1 / 4
Gemin 2 Antibody (MA1-5777) in ICC/IF
Immunofluorescence analysis of Gemin 2 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Gemin 2 Mouse Monoclonal Antibody (Product # MA1-5777) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at... View More
Product Details
MA1-5777
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Xenopus
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
2E17
Immunogen
His-Gemin 2 fusion protein (amino acids 12 - 280, starting at 2nd methionine)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2187799
Product Specific Information
MA1-5777 detects Gemin2 in human and xenopus samples.
MA1-5777 has been successfully used in Western blot, immunoprecipitation, immunofluorescence and ELISA procedures. MA1-5777 detects a band of approximately 34 kDa.
The MA1-5777 immunogen is His-Gemin2 (starting at 2nd methionine).
Store at 4ºC for one month, or in small aliquots at -20ºC for longer periods.
Target Information
SIP1 is one of the proteins found in the SMN complex, which consists of the survival of motor neuron (SMN) protein and several gemin proteins. The SMN complex is localized to a subnuclear compartment called gems (gemini of coiled bodies) and is required for assembly of spliceosomal snRNPs and for pre-mRNA splicing. SIP1 interacts directly with the SMN and it is required for formation of the SMN complex. A knockout mouse targeting the mouse homolog of this gene exhibited disrupted snRNP assembly and motor neuron degeneration. However, knockdown of the SIP1 mRNA in motor neurons showed normal motor axons while that of SMN mRNA did show abnormal motor axon outgrowth, indicating that SIP1 may have additional roles outside of the SMN complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Component of gems 2; gem (nuclear organelle) associated protein 2; Gem-associated protein 2; Gemin-2; SIP 1; SIP-1; SIP1 delta; SMN interacting protein 1-delta; SMN-interacting protein 1; survival of motor neuron protein interacting protein 1; Survival of motor neuron protein-interacting protein 1; survivor of motor neuron protein interacting protein 1
Gene Aliases: 1700012N19Rik; GEMIN2; SIP1; SIP1-delta; XELAEV_18042638mg
UniProt ID: (Human) O14893, (Mouse) Q9CQQ4
Entrez Gene ID: (Human) 8487, (Mouse) 66603, (Xenopus) 494588
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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