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Invitrogen
Gemin 4 Polyclonal Antibody
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FIGURE: 1 / 1
Gemin 4 Antibody (PA5-88031) in WB
Western blot analysis of Gemin 4 in extracts of various cell lines. Samples were incubated with Gemin 4 Polyclonal antibody (Product # PA5-88031) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-88031
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 50-350 of human GEMIN4 (NP_0565362)
Conjugate
Form
Liquid
Concentration
3.63 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2804602
Product Specific Information
Immunogen sequence: LREISSAAAH SQPFAWKKKA LIIIWAKVLQ PHPVTPSDTE TRWQEDLFFS VGNMIPTINH TILFELLKSL EASGLFIQLL MALPTTICHA ELERFLEHVT VDTSAEDVAF FLDVWWEVMK HKGHPQDPLL SQFSAMAHKY LPALDEFPHP PKRLRSDPDA CPTMPLLAML LRGLTQIQSR ILGPGRKCCA LANLADMLTV FALTEDDPQE VSATVYLDKL ATVISVWNSD TQNPYHQQAL AEKVKEAERD VSLTSLAKLP SETIFVGCEF LHHLLREWGE ELQAVLRSSQ GTSYDSYRLC D; Positive Samples: HeLa, MCF-7, SH-SY5Y, Jurkat, Mouse brain, Mouse kidney, Mouse liver; Cellular Location: Cytoplasm, Nucleus, gem, nucleolus
Target Information
The product of this gene is part of a large complex localized to the cytoplasm, nucleoli, and to discrete nuclear bodies called Gemini bodies. The complex functions in spliceosomal snRNP assembly in the cytoplasm, and regenerates spliceosomes required for pre-mRNA splicing in the nucleus. The encoded protein directly interacts with a DEAD box protein and several spliceosome core proteins. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Component of gems 4; DKFZp434B131; DKFZp434D174; Gem-associated protein 4; Gemin-4; HCC-associated protein 1; p97
Gene Aliases: 4932415L08Rik; AU015359; GEMIN4; HC56; HCAP1; HHRF-1; p97
UniProt ID: (Human) P57678
Entrez Gene ID: (Human) 50628, (Mouse) 276919
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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