Rockland

Gli2 Polyclonal Antibody

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

600-401-695

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500- 1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

2-20 µg/mL
-

ELISA (ELISA)

1:15,000-1:60,000
-

Immunoprecipitation (IP)

1:500-1:2,000
-
Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids from an internal region of Mouse Gli-2.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.02 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Directed against mouse Gli-2 protein. A BLAST analysis was used to suggest cross-reactivity with Gli-2 from mouse and rat sources based on 100% sequence homology with the immunogen. Reactivity with Gli-2 from other sources is not known.

Target Information

Gli-2 belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Tax helper protein; Zinc finger protein GLI2

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Gene Aliases: AW546128; Gli2; Thp

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UniProt ID: (Mouse) Q0VGT2

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Entrez Gene ID: (Mouse) 14633

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding nucleic acid binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding zinc ion binding sequence-specific DNA binding transcription regulatory region DNA binding metal ion binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter skeletal system development osteoblast differentiation in utero embryonic development kidney development morphogenesis of an epithelium chondrocyte differentiation osteoblast development transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter smoothened signaling pathway pattern specification process axon guidance ventral midline development hindgut morphogenesis heart development cell proliferation positive regulation of cell proliferation regulation of smoothened signaling pathway epidermal cell differentiation dorsal/ventral pattern formation proximal/distal pattern formation floor plate formation spinal cord dorsal/ventral patterning ventral spinal cord development spinal cord motor neuron differentiation cerebellar cortex morphogenesis smoothened signaling pathway involved in ventral spinal cord interneuron specification smoothened signaling pathway involved in spinal cord motor neuron cell fate specification dorsal/ventral neural tube patterning neural tube development smoothened signaling pathway involved in regulation of cerebellar granule cell precursor cell proliferation spinal cord ventral commissure morphogenesis pituitary gland development cell differentiation lung development mammary gland development hindbrain development negative regulation of chondrocyte differentiation tube development odontogenesis of dentin-containing tooth embryonic digit morphogenesis positive regulation of neuron differentiation positive regulation of DNA replication positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter embryonic digestive tract development developmental growth anatomical structure formation involved in morphogenesis neuron development branching morphogenesis of an epithelial tube anatomical structure development notochord regression head development prostatic bud formation mammary gland duct morphogenesis smoothened signaling pathway involved in dorsal/ventral neural tube patterning cellular response to organic cyclic compound cochlea morphogenesis cellular response to virus
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