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Glis1 Antibody (A303-676A) in WB
Detection of human Glis1 by western blot. Samples: Whole cell lysate from HeLa (15 and 50 µg), HEK293T (T; 50 µg) and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-Glis1 antibody (Product # A303-676A) used for WB at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
Product Details
A303-676A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 570 and 620 of human GLIS family zinc finger 1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
Glis1 (Gli similar 1) is also known as Gli related Kruppel-like zinc finger 1 and is encoded by the Glis1 gene located on chromosome 1 in humans. It belongs to the Kruppel-like zinc finger family of proteins that constitute mainly of transcription factors and is hence studied for its role in target gene activation and repression, specifically during embryonic development. Glis1 contains five tandem zinc finger motifs, which is the region at which it shares close homology to Glis2 and Glis 3. More recent studies on Glis1 show its interaction with Oct 3/4, Sox2 and KLF4 in efficient generation of iPSC.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GLI-similar 1; GLIS1; Zinc finger protein GLIS1
Gene Aliases: GLIS1
UniProt ID: (Human) Q8NBF1
Entrez Gene ID: (Human) 148979
RNA polymerase II regulatory region sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
metal ion binding
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