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FIGURE: 1 / 3
Globo H Antibody (14-9700-82) in IHC (P)
Immunohistochemistry of formalin-fixed paraffin embedded human breast cancer tissue stained with 5 µg/mL of Mouse IgG3 K Isotype Control Purified (left) or 5 µg/mL of Anti-Human Globo H Purified (right) followed by Anti-Mouse IgG Biotin, Streptavidin HRP, and DAB visualization.Nuclei are counterstained with hematoxylin.
Product Details
14-9700-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG3, kappa
Class
Monoclonal
Type
Antibody
Clone
VK9
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with proprietary stabilizer
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2572961
Product Specific Information
Description: The VK9 monoclonal antibody recognizes human Globo H, a hexasaccharide formed from the pentasaccharide precursor, SSEA3 (stage-specific embryonic antigen 3). It is a member of a family of antigenic carbohydrates expressed on the surface of cancer cells as glycolipids and possibly glycoproteins. Globo H is overexpressed on many carcinomas, such as breast, colon, ovarian, gastric, pancreatic, lung, and prostate. Expression is observed in more than half of all ductal, lobular, and tubular breast carcinomas, but not in non-epithelial breast tumors. Globo H is also expressed on cancer stem cells and human embryonic stem cells, although expression decreases when cells are differentiated toward both neural progenitor cells and to a lesser extent in endodermal lineage cells. Expression in normal tissues is limited to cells composing secretory borders. Globo H is considered a target for immunotherapy of many epithelial cancers.
Applications Reported: This VK9 antibody has been reported for use in flow cytometric analysis, western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and microscopy.
Applications Tested: This VK9 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using either high or low pH antigen retrieval at less than or equal to 5 µg/mL. The VK9 antibody has also been tested by western blot of reduced MCF-7 cell lysate and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Globo H is a hexasaccharide formed from the pentasaccharide precursor, SSEA3 (stage-specific embryonic antigen 3). It is a member of a family of antigenic carbohydrates expressed on the surface of cancer cells as glycolipids and possibly glycoproteins. Globo H is overexpressed on many carcinomas, such as breast, colon, ovarian, gastric, pancreatic, lung, and prostate. Expression is observed in more than half of all ductal, lobular, and tubular breast carcinomas, but not in non-epithelial breast tumors. Globo H is also expressed on cancer stem cells and human embryonic stem cells, although expression decreases when cells are differentiated toward both neural progenitor cells and to a lesser extent in endodermal lineage cells. Expression in normal tissues is limited to cells composing secretory borders. Globo H is considered a target for immunotherapy of many epithelial cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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