Invitrogen

GluR3 Monoclonal Antibody (3B3)

Advanced Verification

6 Published Figures

9 References

View all (15) GluR3 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (6)
Advanced Verification (2)

FIGURE: 1 / 8

GluR3 Antibody (32-0400) in WB

iScience 2023 - Generation and molecular analysis of global double GluA1 and GluA3 knockout mice ( Gria1/3 -/- ) and constitutive mutants ( Gria1/3 DeltaFb ) with specific loss of the GluA1 and GluA3 subunits in excitatory forebrain neurons (A) Gria1/3 -/- were generated by matings of GluA1 and GluA3 knockout mice. A three-week-old Gria1/3 -/- mouse raised by a foster mother can be recognized by its smaller body size and i... View More

Product Image Gallery

GluR3 Antibody — Figure 4. A , B , Changes in AMPA receptor subunit expression of the AMPA receptor subunits GluR-A, GluR-B, GluR-C, and GluR-D in hippocampus ( A ) and cerebellum ( B ) of wild-type (WT) and GluR-C knock-out (GluR-C -/-) mice from P2 to P90. Total proteins of the hippocampus and the cerebellum were isolated, and for each sample, 10 mug of protein was separated on a 7.5% SDS-PAGE and transferred on nitrocellulose. The glutamate receptor subunits were visualized by selective antibodies and monitored by autoradiography. The alpha-subunit of the Ca 2+ /calmodulin-dependent protein kinase II (alpha-CaMKII) and betaIII-tubulin (betaIII-Tub.) was used as a positive control for developmental controlled gene expression in hippocampus and cerebellum, respectively. Loading was controlled by beta-actin. The experiment was repeated with at least three sets of mice. One representative example for each subunit is given. {KO}

Product Details

32-0400

Applications

Tested Dilution

Publications

Western Blot (WB)

5 µg/mL

View 8 publications 8 publications

Immunocytochemistry (ICC/IF)

1-3 µg/mL

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Non-human primate, Rat

Published species

Mouse, Rat, Tag

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

3B3

Immunogen

Fusion protein encompassing the N-terminal region of rat GluR3.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2533059

Target Information

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA->GGA; R->G). Alternative splicing at this locus results in different isoforms, which may vary in their signal transduction properties.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Distinct effects of AMPAR subunit depletion on spatial memory.

iScience

Eltokhi A,Bertocchi I,Rozov A,Jensen V,Borchardt T,Taylor A,Proenca CC,Rawlins JNP,Bannerman DM,Sprengel R

Published figure using GluR3 monoclonal antibody (Product # 32-0400) in Western Blot

Fri Nov 17 00:00:00 UTC 2023

Hippocampal AMPA receptor assemblies and mechanism of allosteric inhibition.

Nature

Yu J,Rao P,Clark S,Mitra J,Ha T,Gouaux E

32-0400 was used in Western Blot to demonstrate how the receptor-TARP-γ8 stoichiometry explains the mechanism of and submaximal inhibition by a clinically relevant, brain-region-specific allosteric inhibitor.

Tue Jun 01 00:00:00 UTC 2021

Cognitive aging is associated with redistribution of synaptic weights in the hippocampus.

Proceedings of the National Academy of Sciences of the United States of America

Buss EW,Corbett NJ,Roberts JG,Ybarra N,Musial TF,Simkin D,Molina-Campos E,Oh KJ,Nielsen LL,Ayala GD,Mullen SA,Farooqi AK,D'Souza GX,Hill CL,Bean LA,Rogalsky AE,Russo ML,Curlik DM,Antion MD,Weiss C,Chetkovich DM,Oh MM,Disterhoft JF,Nicholson DA

32-0400 was used in Western Blot to show that the synaptic architecture of hippocampal regions CA1 and CA3 is maintained in a young adult-like state in aged rats that performed comparably to their young adult counterparts in both trace eyeblink conditioning and Morris water maze learning.

Tue Feb 23 00:00:00 UTC 2021

Cognitive aging is associated with redistribution of synaptic weights in the hippocampus.

Proceedings of the National Academy of Sciences of the United States of America

Tue Feb 23 00:00:00 UTC 2021

L-Stepholidine rescues memory deficit and synaptic plasticity in models of Alzheimer's disease via activating dopamine D1 receptor/PKA signaling pathway.

Cell death & disease

Hao JR,Sun N,Lei L,Li XY,Yao B,Sun K,Hu R,Zhang X,Shi XD,Gao C

32-0400 was used in western blot to utilize models of Alzheimer's disease via activating dopamine D1 receptor/PKA signaling pathway where L-Stepholidine rescues memory deficit and synaptic plasticity

Thu Nov 05 00:00:00 UTC 2015

Differential trafficking of AMPA and NMDA receptors during long-term potentiation in awake adult animals.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Williams JM,Guévremont D,Mason-Parker SE,Luxmanan C,Tate WP,Abraham WC

Wed Dec 19 00:00:00 UTC 2007

Involvement of the AMPA receptor GluR-C subunit in alcohol-seeking behavior and relapse.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Sanchis-Segura C,Borchardt T,Vengeliene V,Zghoul T,Bachteler D,Gass P,Sprengel R,Spanagel R

Wed Jan 25 00:00:00 UTC 2006

Involvement of the AMPA receptor GluR-C subunit in alcohol-seeking behavior and relapse.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Sanchis-Segura C,Borchardt T,Vengeliene V,Zghoul T,Bachteler D,Gass P,Sprengel R,Spanagel R

Wed Jan 25 00:00:00 UTC 2006

Involvement of the AMPA receptor GluR-C subunit in alcohol-seeking behavior and relapse.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Sanchis-Segura C,Borchardt T,Vengeliene V,Zghoul T,Bachteler D,Gass P,Sprengel R,Spanagel R

Wed Jan 25 00:00:00 UTC 2006

A juvenile form of postsynaptic hippocampal long-term potentiation in mice deficient for the AMPA receptor subunit GluR-A.

The Journal of physiology

Jensen V,Kaiser KM,Borchardt T,Adelmann G,Rozov A,Burnashev N,Brix C,Frotscher M,Andersen P,Hvalby Ø,Sakmann B,Seeburg PH,Sprengel R

Mon Dec 15 00:00:00 UTC 2003

Glutamate receptor subunit 3 (GluR3) immunoreactivity delineates a subpopulation of parvalbumin-containing interneurons in the rat hippocampus.

The Journal of comparative neurology

Moga DE,Janssen WG,Vissavajjhala P,Czelusniak SM,Moran TM,Hof PR,Morrison JH

Mon Jul 14 00:00:00 UTC 2003

Glutamate receptor subunit 3 (GluR3) immunoreactivity delineates a subpopulation of parvalbumin-containing interneurons in the rat hippocampus.

The Journal of comparative neurology

Moga DE,Janssen WG,Vissavajjhala P,Czelusniak SM,Moran TM,Hof PR,Morrison JH

Mon Jul 14 00:00:00 UTC 2003

Bioinformatics

Protein Aliases: AMPA-selective glutamate receptor 3; dJ1171F9.1; GluR-3; GluR-C; GluR-K3; glutamate receptor; Glutamate receptor 3; glutamate receptor C; Glutamate receptor ionotropic, AMPA 3; glutamate receptor subunit 3; glutamate receptor, ionotrophic, AMPA 3; glutamate receptor, ionotropic, AMPA 3; glutamate receptor, ionotropic, AMPA3 (alpha 3); OTTHUMP00000024262; RP11-349N19.3

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Gene Aliases: GluA3; GluR-3; GLUR-C; GLUR-K3; GLUR3; GLURC; GRIA3; MRX94

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UniProt ID: (Human) P42263, (Rat) P19492

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Entrez Gene ID: (Human) 2892, (Rat) 29628

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Function(s)

AMPA glutamate receptor activity excitatory extracellular ligand-gated ion channel activity extracellular-glutamate-gated ion channel activity ionotropic glutamate receptor activity protein binding PDZ domain binding

Process(es)

regulation of receptor recycling response to lithium ion ion transmembrane transport ionotropic glutamate receptor signaling pathway synaptic transmission, glutamatergic response to fungicide transport glutamate receptor signaling pathway chemical synaptic transmission

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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GluR3 Monoclonal Antibody (3B3)

GluR3 Antibody (32-0400) in WB

Product Details

32-0400

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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