Proteintech

Glucagon Polyclonal Antibody

Glucagon Antibody in Immunocytochemistry (ICC/IF)

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Glucagon Antibody (15954-1-AP) in ICC/IF

Immunofluorescent analysis of (4% PFA) fixed human pancreas tissue using (Product # 15954-1-AP) (Glucagon antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The B cells of the islets of Langerhans was labelled in red with 66198-1-Ig (INS antibody).

Glucagon Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Glucagon Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

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Product Details

15954-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

Immunohistochemistry (IHC)

View 9 publications 9 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 20 publications 20 publications

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Glucagon Fusion Protein Ag8677 (1-180 aa encoded by BC005278)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.24 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MKSIYFVAG LFVMLVQGSW QRSLQDTEEK SRSFSASQAD PLSDPDQMNE DKRHSQGTFT SDYSKYLDSR RAQDFVQWLM NTKRNRNNIA KRHDEFERHA EGTFTSDVSS YLEGQAAKEF IAWLVKGRGR RDFPEEVAIV EELGRRHADG SFSDEMNTIL DNLAARDFIN WLIQTKITDR K (1-180 aa encoded by BC005278)

Target Information

Glucagon is a 29-residue polypeptide hormone (MW 3482), produced in the pancreas. A related hormone, enteroglucagon (or oxyntomodulin), which is produced in the mucosa of the small and large intestine, consists of the 29 amino acid sequence of pancreatic glucagon extended by 8 additional residues at the C-terminus. The biological activities of pancreatic glucagon include glycogenolysis, lipolysis, gluconeogenesis, and ketogensis, which are antagonistic effects to those of insulin action, thus leading to increased blood glucose levels. Immunocytochemical studies have revealed the presence of pancreatic glucagon inside the A or alpha cells, which constitute 15-20% of the islet cell population. These cells are located preferentially at the periphery of the human pancreatic islets. Pathological manifestations of the glucagon-type peptide residue almost exclusively with the exsistence of tumors or glucagonomas, as no states of glucagon-cell deficiency or hyperplasia have been identified. Glucagon-specific antibodies would prove useful as a cell and tumor markers applying immunohistochemical techniques, and as an analytical tool in qualification of the hormone.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Paternal sleep deprivation induces metabolic perturbations in male offspring via altered LRP5 DNA methylation of pancreatic islets.

Journal of pineal research

Zeng Y,Zhang Z,Liang S,Chang X,Qin R,Chen H,Guo L

Mon May 01 00:00:00 UTC 2023

Fufang-zhenzhu-tiaozhi formula protects islet against injury and promotes β cell regeneration in diabetic mice.

Journal of ethnopharmacology

Chen X,Yin J,Zhong Q,Wang K,Zhang X,Liang M,Lin Q,Wang H,Wang W,Wang L,Hu X,Bei W,Guo J

Mon Jan 30 00:00:00 UTC 2023

FTZ promotes islet β-cell regeneration in T1DM mice via the regulation of nuclear proliferation factors.

Journal of ethnopharmacology

Zhong Q,Yin J,Wang K,Chen X,Wang H,Hu X,Wang W,Wang L,Bei W,Guo J

Sat Oct 28 00:00:00 UTC 2023

Combined therapy of GABA and sitagliptin prevents high-fat diet impairment of beta-cell function.

Molecular and cellular endocrinology

Wang Z,Fan L,Ni Y,Wu D,Ma A,Zhao Y,Li J,Cui Q,Zhou Y,Zhang L,Lou YR,Prud'homme GJ,Wang Q

Sun Jan 01 00:00:00 UTC 2023

VAMP4 regulates insulin levels by targeting secretory granules to lysosomes.

The Journal of cell biology

Li M,Feng F,Feng H,Hu P,Xue Y,Xu T,Song E

Mon Oct 03 00:00:00 UTC 2022

Type IIB PKA is highly expressed in β cells and controls cell proliferation via regulating Cyclin D1 expression.

The FEBS journal

Jiang Y,Zhu L,Wu D,Ni Y,Huang C,Ye H,Yang Y,Liu R,Li Y

Sun May 01 00:00:00 UTC 2022

A Chinese medical nutrition therapy diet accompanied by intermittent energy restriction alleviates type 2 diabetes by enhancing pancreatic islet function and regulating gut microbiota composition.

Food research international (Ottawa, Ont.)

Luo W,Zhou J,Yang X,Wu R,Liu H,Shao H,Huang B,Kang X,Yang L,Liu D

Tue Nov 01 00:00:00 UTC 2022

cGAS-STING mediates cytoplasmic mitochondrial-DNA-induced inflammatory signal transduction during accelerated senescence of pancreatic β-cells induced by metabolic stress.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Hu H,Zhao R,He Q,Cui C,Song J,Guo X,Zang N,Yang M,Zou Y,Yang J,Li J,Wang L,Xia L,Wang L,He F,Hou X,Yan F,Chen L

Sun May 01 00:00:00 UTC 2022

Small Hepatitis B Virus Surface Antigen Promotes Hepatic Gluconeogenesis via Enhancing Glucagon/cAMP/Protein Kinase A/CREB Signaling.

Journal of virology

Chen Y,Wang B,Ou X,Wu Y,He Y,Lin X,Lin X

Wed Dec 14 00:00:00 UTC 2022

Long-Term High-Fat High-Fructose Diet Induces Type 2 Diabetes in Rats through Oxidative Stress.

Nutrients

Zhao Y,Wang QY,Zeng LT,Wang JJ,Liu Z,Fan GQ,Li J,Cai JP

Tue May 24 00:00:00 UTC 2022

The potential mechanism of Liu-Wei-Di-Huang Pills in treatment of type 2 diabetic mellitus: from gut microbiota to short-chain fatty acids metabolism.

Acta diabetologica

Yi ZY,Chen L,Wang Y,He D,Zhao D,Zhang SH,Yu R,Huang JH

Sat Oct 01 00:00:00 UTC 2022

SARS-CoV-2 infects human pancreatic β cells and elicits β cell impairment.

Cell metabolism

Wu CT,Lidsky PV,Xiao Y,Lee IT,Cheng R,Nakayama T,Jiang S,Demeter J,Bevacqua RJ,Chang CA,Whitener RL,Stalder AK,Zhu B,Chen H,Goltsev Y,Tzankov A,Nayak JV,Nolan GP,Matter MS,Andino R,Jackson PK

Tue Aug 03 00:00:00 UTC 2021

Inhibition of Notch activity promotes pancreatic cytokeratin 5-positive cell differentiation to beta cells and improves glucose homeostasis following acute pancreatitis.

Cell death & disease

Zhang X,Tao J,Yu J,Hu N,Zhang X,Wang G,Feng J,Xiong X,Li M,Chai D,Li H,Rong Y,Tang Z,Wang W,Peng Z,Shi Q

Thu Sep 23 00:00:00 UTC 2021

JunD Regulates Pancreatic β-Cells Function by Altering Lipid Accumulation.

Frontiers in endocrinology

Wang K,Cui Y,Lin P,Yao Z,Sun Y

Thu Dec 23 00:00:00 UTC 2021

Nfe2l1 deficiency mitigates streptozotocin-induced pancreatic β-cell destruction and development of diabetes in male mice.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Bao S,Zheng H,Chen C,Zhang Y,Bao L,Yang B,Hou Y,Chen Y,Zhang Q,Pi J,Fu J

Wed Dec 01 00:00:00 UTC 2021

The active GLP-1 analogue liraglutide alleviates H9N2 influenza virus-induced acute lung injury in mice.

Microbial pathogenesis

Bai Y,Lian P,Li J,Zhang Z,Qiao J

Fri Jan 01 00:00:00 UTC 2021

Mesenchymal stem cell-conditioned medium alleviates high fat-induced hyperglucagonemia via miR-181a-5p and its target PTEN/AKT signaling.

Molecular and cellular endocrinology

Song J,He Q,Guo X,Wang L,Wang J,Cui C,Hu H,Yang M,Cui Y,Zang N,Yan F,Liu F,Sun Y,Liang K,Qin J,Zhao R,Wang C,Sun Z,Hou X,Li W,Chen L

Mon Nov 01 00:00:00 UTC 2021

Mof acetyltransferase inhibition ameliorates glucose intolerance and islet dysfunction of type 2 diabetes via targeting pancreatic α-cells.

Molecular and cellular endocrinology

Guo X,Cui C,Song J,He Q,Zang N,Hu H,Wang X,Li D,Wang C,Hou X,Li X,Liang K,Yan F,Chen L

Mon Nov 01 00:00:00 UTC 2021

Genetic activation of α-cell glucokinase in mice causes enhanced glucose-suppression of glucagon secretion during normal and diabetic states.

Molecular metabolism

Bahl V,Lee May C,Perez A,Glaser B,Kaestner KH

Thu Jul 01 00:00:00 UTC 2021

Disrupted glucose homeostasis and skeletal-muscle-specific glucose uptake in an exocyst knockout mouse model.

The Journal of biological chemistry

Fujimoto BA,Young M,Nakamura N,Ha H,Carter L,Pitts MW,Torres D,Noh HL,Suk S,Kim JK,Polgar N

Fri Aug 27 00:00:00 UTC 2021

Morphological and biochemical changes in the pancreas associated with acute systemic hypoxia.

Human cell

Morioka F,Tani N,Ikeda T,Hirokawa T,Ikeda K,Shida A,Aoki Y,Ishikawa T

Mon Mar 01 00:00:00 UTC 2021

HGF can reduce accumulation of inflammation and regulate glucose homeostasis in T2D mice.

Journal of physiology and biochemistry

Yan L,He X,Tang Y,Zhao X,Luo G,Wang X

Mon Nov 01 00:00:00 UTC 2021

Multifaceted secretion of htNSC-derived hypothalamic islets induces survival and antidiabetic effect via peripheral implantation in mice.

eLife

Tang Y,Zuniga-Hertz JP,Han C,Yu B,Cai D

Fri Feb 21 00:00:00 UTC 2020

Mint3 is dispensable for pancreatic and kidney functions in mice.

Biochemistry and biophysics reports

Chung Y,Saitoh Y,Hayashi T,Fukui Y,Terada N,Seiki M,Murakami Y,Sakamoto T

Tue Dec 01 00:00:00 UTC 2020

aThe characteristics of glucose metabolism in the sulfonylurea receptor 1 knockout rat model.

Molecular medicine (Cambridge, Mass.)

Zhou X,Xu C,Zou Z,Shen X,Xie T,Zhang R,Liao L,Dong J

Mon Jan 07 00:00:00 UTC 2019

aThe characteristics of glucose metabolism in the sulfonylurea receptor 1 knockout rat model.

Molecular medicine (Cambridge, Mass.)

Zhou X,Xu C,Zou Z,Shen X,Xie T,Zhang R,Liao L,Dong J

Mon Jan 07 00:00:00 UTC 2019

Simultaneous Real-Time Measurement of the β-Cell Membrane Potential and Ca²⁺ Influx to Assess the Role of Potassium Channels on β-Cell Function.

Methods in molecular biology (Clifton, N.J.)

Vierra NC,Dickerson MT,Philipson LH,Jacobson DA

Mon Jul 23 00:00:00 UTC 2018

Simultaneous Real-Time Measurement of the β-Cell Membrane Potential and Ca²⁺ Influx to Assess the Role of Potassium Channels on β-Cell Function.

Methods in molecular biology (Clifton, N.J.)

Vierra NC,Dickerson MT,Philipson LH,Jacobson DA

Mon Jul 23 00:00:00 UTC 2018

TALK-1 reduces delta-cell endoplasmic reticulum and cytoplasmic calcium levels limiting somatostatin secretion.

Molecular metabolism

Vierra NC,Dickerson MT,Jordan KL,Dadi PK,Katdare KA,Altman MK,Milian SC,Jacobson DA

Thu Mar 01 00:00:00 UTC 2018

TALK-1 reduces delta-cell endoplasmic reticulum and cytoplasmic calcium levels limiting somatostatin secretion.

Molecular metabolism

Vierra NC,Dickerson MT,Jordan KL,Dadi PK,Katdare KA,Altman MK,Milian SC,Jacobson DA

Thu Mar 01 00:00:00 UTC 2018

SIRT6-mediated transcriptional suppression of Txnip is critical for pancreatic beta cell function and survival in mice.

Diabetologia

Qin K,Zhang N,Zhang Z,Nipper M,Zhu Z,Leighton J,Xu K,Musi N,Wang P

Sun Apr 01 00:00:00 UTC 2018

TALK-1 channels control β cell endoplasmic reticulum Ca²⁺ homeostasis.

Science signaling

Vierra NC,Dadi PK,Milian SC,Dickerson MT,Jordan KL,Gilon P,Jacobson DA

Tue Sep 19 00:00:00 UTC 2017

TALK-1 channels control β cell endoplasmic reticulum Ca²⁺ homeostasis.

Science signaling

Vierra NC,Dadi PK,Milian SC,Dickerson MT,Jordan KL,Gilon P,Jacobson DA

Tue Sep 19 00:00:00 UTC 2017

CNC-bZIP protein Nrf1-dependent regulation of glucose-stimulated insulin secretion.

Antioxidants & redox signaling

Zheng H,Fu J,Xue P,Zhao R,Dong J,Liu D,Yamamoto M,Tong Q,Teng W,Qu W,Zhang Q,Andersen ME,Pi J

Wed Apr 01 00:00:00 UTC 2015

CNC-bZIP protein Nrf1-dependent regulation of glucose-stimulated insulin secretion.

Antioxidants & redox signaling

Zheng H,Fu J,Xue P,Zhao R,Dong J,Liu D,Yamamoto M,Tong Q,Teng W,Qu W,Zhang Q,Andersen ME,Pi J

Wed Apr 01 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: glicentin-related polypeptide; GLP1/2; glucagon; glucagon-like peptide 1; glucagon-like peptide 2; glucagon-like peptide-1; IL12p35; IL12P40; OXY; preproglucagon; Pro-glucagon

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Gene Aliases: GCG; GLP-1; GLP1; GLP2; Glu; GRPP; PPG

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UniProt ID: (Human) P01275, (Mouse) P55095

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Entrez Gene ID: (Human) 2641, (Mouse) 14526, (Rat) 24952

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Function(s)

receptor binding hormone activity protein binding glucagon receptor binding identical protein binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Glucagon Polyclonal Antibody

Glucagon Antibody (15954-1-AP) in ICC/IF

Product Details

15954-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (MISC)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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