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Glucoamylase Antibody (200-101-234-0100) in WB
Rockland Goat anti Glucoamylase antibody (100-101-234 lot 7844) was used to detect purified glucoamylase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 488 conjugated Donkey anti goat (605-741-125 lot 21094 1:10K in TBS/MB-070 1 hr RT) . Ima... View More
Product Details
200-101-234-0100
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Fungi
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Glucoamylase [Rhizopus]
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Ion-exchange chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-GLUCOAMYLASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glucoamylase [Rhizopus]. Cross reactivity against Glucoamylase from other sources is unknown.
Target Information
Rhizopus glucoamylase exists in multiple forms, Gluc 1, Gluc 2, and Gluc 3, all of which hydrolyze gelatinized starch at similar rates, but only the largest one (Gluc 1) is able to adsorb raw starch. Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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