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Invitrogen
Glucocorticoid Receptor Monoclonal Antibody (5E4), FITC
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FIGURE: 1 / 2
Glucocorticoid Receptor Antibody (MA1-81793) in ICC/IF
Immunofluorescence analysis of Glucocorticoid Receptor was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Glucocorticoid Receptor, FITC Mouse Monoclonal Antibody (5E4) (Product # MA1-81793) at 5 µg/mL in 0... View More
Product Details
MA1-81793
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
5E4
Immunogen
26 amino acid peptide corresponding to residues 150-176 on human GCR linked thyroglobulin.
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
0.1 mg/mL
Purification
Ion-exchange chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
<0.1% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_930996
Product Specific Information
Membrane permeabilization is required for flow cytometry applications. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Mouse anti Human Glucocorticoid Receptor antibody, clone 5E4 recognizes the human glucocorticoid receptor, also known as Nuclear receptor subfamily 3 group C member 1 (NR3C1), a 777 amino acid receptor bearing 3 distinct functional domains, an N-terminal modulating domain, a DNA binding domain and a C-terminal steroid binding domain.
Target Information
Glucocorticoid Receptor (NR3C1) is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. Glucocorticoid Receptor can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Glucocorticoid Receptor is expressed in the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. Mutations in the Glucocorticoid Receptor gene are a cause of glucocorticoid resistance, or cortisol, resistance. Studies have shown that glucocorticoid receptor (GR) must be associated with a complex of chaperone proteins for ligand activation. GR binds to known steroids such as dexamethasone with nanomolar affinity
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: glucocorticoid nuclear receptor variant 1; Glucocorticoid receptor; GR; gr alpha; GR Kit; Nuclear receptor subfamily 3 group C member 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor); OTTHUMP00000222854; OTTHUMP00000222858
Gene Aliases: GCCR; GCR; GCRST; GR; GRL; NR3C1
UniProt ID: (Human) P04150
Entrez Gene ID: (Human) 2908
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
RNA binding
glucocorticoid receptor activity
steroid binding
protein binding
zinc ion binding
protein complex binding
glucocorticoid-activated RNA polymerase II transcription factor binding transcription factor activity
steroid hormone binding
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
transcription initiation from RNA polymerase II promoter
apoptotic process
chromosome segregation
mitotic nuclear division
signal transduction
covalent chromatin modification
glucocorticoid receptor signaling pathway
glucocorticoid mediated signaling pathway
positive regulation of transcription from RNA polymerase II promoter
cell division
cellular response to steroid hormone stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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