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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Glucocorticoid Receptor beta Antibodies

          Invitrogen

          Glucocorticoid Receptor beta Polyclonal Antibody

          6 Published Figures
          35 References
          View all (2) Glucocorticoid Receptor beta antibodies

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          Datasheet
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          Datasheet
          Protocols
          Questions & Answers

          Cite Glucocorticoid Receptor beta Polyclonal Antibody

          • Antibody Testing Data (1)
          • Published Figures (6)
          Glucocorticoid Receptor beta Antibody in Western Blot (WB)
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          Glucocorticoid Receptor beta Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 7

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          Glucocorticoid Receptor beta Antibody (PA3-514) in WB

          Western blot of hGR beta transfected COS-7 whole cell extract using Product # PA3-514. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Glucocorticoid Receptor beta Antibody in Western Blot (WB)
          Glucocorticoid Receptor beta Antibody in Immunohistochemistry (IHC)
          Glucocorticoid Receptor beta Antibody in Immunohistochemistry (IHC)
          Glucocorticoid Receptor beta Antibody in Western Blot (WB)
          Glucocorticoid Receptor beta Antibody in Western Blot (WB)
          Glucocorticoid Receptor beta Antibody in Western Blot (WB)
          Glucocorticoid Receptor beta Antibody in Western Blot (WB)

          Product Details

          PA3-514

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500
          View 18 publications 18 publications

          Immunohistochemistry (IHC)

          -
          View 9 publications 9 publications

          Immunocytochemistry (ICC/IF)

          1:1,500
          View 3 publications 3 publications

          Flow Cytometry (Flow)

          -
          View 3 publications 3 publications

          Immunoprecipitation (IP)

          Assay-dependent
          -

          Gel Shift (GS)

          -
          View 2 publications 2 publications
          Product Specifications

          Species Reactivity

          Human

          Published species

          Human, Mouse, Pig, Sheep

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic Peptide: N(728) V M W L K P E S T S H T L I(742) C
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          Conc. Not Determined

          Storage buffer

          whole serum, PBS

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2155928

          Product Specific Information

          PA3-514 detects glucocorticoid receptor (GR) beta from human cells.

          PA3-514 has been successfully used in Western blot, immunocytochemistry and immunoprecipitation procedures. By Western blot, this antibody detects a ~90 kDa protein representing GR beta from HeLa cell lysate. Immunocytochemical staining of GR beta in HeLa cells with PA3-514 results in nuclear staining.

          PA3-514 immunizing peptide corresponds to amino acid residues 728-742 from human GR beta. This peptide (Cat. # PEP-222) is available for use in neutralization and control experiments.

          Target Information

          Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism, and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocorticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain GR. The human GR exists in two forms, alpha and beta, which are thought to be the result of alternative splicing of a single gene. Sequence analysis indicates that the alpha and beta forms of human GR are 777 and 742 amino acids long, respectively. They are identical up to residue 727, after which they diverge. After ligand binding, the 94 kDa GR alpha isoform has been observed to translocate from the cytoplasm to the nucleus where it regulates gene expression. In contrast, the 90 kDa GR beta isoform does not appear to bind either glucocorticoid agonists or antagonists, and has been localized predominantly in the nucleus independent of hormone treatment in some human cell lines. Studies suggest that human GR beta might function as a dominant negative inhibitor of GR alpha activity.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: glucocorticoid nuclear receptor variant 1; Glucocorticoid receptor; GR; GR Beta; Nuclear receptor subfamily 3 group C member 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)

          View more View less

          Gene Aliases: GCCR; GCR; GCRST; GR; GRL; NR3C1

          View more View less

          UniProt ID: (Human) P04150

          View more View less

          Entrez Gene ID: (Human) 2908

          View more View less

          Function(s)
          RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding RNA binding glucocorticoid receptor activity steroid binding protein binding zinc ion binding protein complex binding glucocorticoid-activated RNA polymerase II transcription factor binding transcription factor activity steroid hormone binding
          Process(es)
          transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter transcription initiation from RNA polymerase II promoter apoptotic process chromosome segregation mitotic nuclear division signal transduction covalent chromatin modification glucocorticoid receptor signaling pathway glucocorticoid mediated signaling pathway positive regulation of transcription from RNA polymerase II promoter cell division cellular response to steroid hormone stimulus
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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