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Invitrogen
Glycerol kinase Polyclonal Antibody
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Glycerol kinase Antibody (PA5-95483) in IHC (P)
Immunohistochemistry analysis of Glycerol kinase in paraffin-embedded mouse kidney tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with Glycerol kinase polyclonal antibody (Product # PA5-95483) at a 1 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Bi... View More
Product Details
PA5-95483
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human Glycerol kinase recombinant protein (Position: M1-S180).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2807285
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human placenta tissue, human HepG2 whole cell, human SW579 whole cell, human SK-OV-3 whole cell, human U-87MG whole cell, rat testis tissue. IHC: mouse kidney tissue, rat kidney tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
The product of this gene belongs to the FGGY kinase family of proteins and encodes glycerol kinase. Glycerol kinase is a key enzyme in the regulation of glycerol uptake and metabolism. It catalyzes the phosphorylation of glycerol by ATP, yielding ADP and glycerol-3-phosphate. Defects in this gene are the cause of glycerol kinase deficiency (GKD). Alternatively spliced transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ASTP; ATP-stimulated glucocorticoid-receptor translocation promoter; ATP-stimulated glucocorticoid-receptor translocaton promoter; ATP:glycerol 3-phosphotransferase; EC 2.7.1.30; GK 3; GK3P; GKl; GKP3; GKTB; Glycerokinase; Glycerokinase 3; Glycerol kinase; Glycerolkinase
Gene Aliases: ASTP; D930012N15Rik; GK; GK1; GKD; Gyk
UniProt ID: (Human) P32189, (Rat) Q63060, (Mouse) Q64516
Entrez Gene ID: (Human) 2710, (Rat) 79223, (Mouse) 14933
glycerol metabolic process
triglyceride metabolic process
response to cold
phosphorylation
regulation of fatty acid metabolic process
triglyceride biosynthetic process
glycerol catabolic process
response to drug
glucose homeostasis
response to ethanol
glycerol-3-phosphate biosynthetic process
glycerol-3-phosphate metabolic process
response to organic substance
carbohydrate metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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