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Glycine Receptor alpha 1 Antibody (AGR-001-200UL) in IHC
Expression of GlyR alpha 1 in rat cerebellum - Immunohistochemical staining of rat cerebellum using Anti-Glycine receptor alpha 1 Antibody (#AGR-001), (1:100). A. GlyR alpha 1 staining (red) is revealed in the molecular layer (MOL), Bergmann glia (horizontal arrow) and in Purkinje cells (a vertical arrow). B. Nuclear staining using DAPI as the counterstain (blue). C. Merged images of A and B.
Product Details
AGR-001-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
(C)RHHKSPMLNLFQD, corresponding to amino acid residues 350-362 of rat GlyR alpha 1, 2ndintracellular loop
Conjugate
Form
Lyophilized
Concentration
0.8 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
GLRA1 is a subunit of a pentameric inhibitory glycine receptor, which mediates postsynaptic inhibition in the central nervous system. Defects in GLRA1 are a cause of startle disease (STHE), also known as hereditary hyperekplexia or congenital stiff-person syndrome. Multiple transcript variants encoding different isoforms have been found for GLRA1. Glycine receptors (GlyR) that include GLRA1 are members of the cys-loop family of ligand-gated ion channels, responsible for mediating the inhibitory effects of glycine. GLRA1 are widely distributed throughout the CNS, particularly within the hippocampus, spinal cord and brain stem. Diseases associated with GLRA1 include Hyperekplexia, Hereditary 1 and Hyperekplexia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GLRA; Glra2; glycine receptor; Glycine receptor 48 kDa subunit; Glycine receptor strychnine-binding subunit; Glycine receptor subunit alpha-1; glycine receptor, alpha 1 subunit; gra1
Gene Aliases: Glra1; Glyr; GLYRA1; nmf11; oscillator; ot; spasmodic; spd
UniProt ID: (Mouse) Q64018, (Rat) P07727
Entrez Gene ID: (Mouse) 14654, (Rat) 25674
action potential
startle response
regulation of respiratory gaseous exchange by neurological system process
transport
ion transport
anion transport
chloride transport
muscle contraction
neuropeptide signaling pathway
chemical synaptic transmission
acrosome reaction
visual perception
adult walking behavior
ion transmembrane transport
regulation of membrane potential
regulation of respiratory gaseous exchange
neuromuscular process controlling posture
neuromuscular process
protein homooligomerization
protein heterooligomerization
negative regulation of transmission of nerve impulse
synaptic transmission, glycinergic
righting reflex
inhibitory postsynaptic potential
chloride transmembrane transport
positive regulation of acrosome reaction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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