Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Glypican 1 Polyclonal Antibody
Promotion
View available promotion(s)
Promo code: {{promo.promoCode}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 1
Glypican 1 Antibody (PA5-88920) in WB
Western blot analysis of Glypican 1 in extracts of various cell lines. Samples were incubated with Glypican 1 Polyclonal antibody (Product # PA5-88920) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
PA5-88920
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 290-420 of human GPC1 (NP_0020722)
Conjugate
Form
Liquid
Concentration
1.4 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805221
Product Specific Information
Immunogen sequence: WRNLLDSMVL ITDKFWGTSG VESVIGSVHT WLAEAINALQ DNRDTLTAKV IQGCGNPKVN PQGPGPEEKR RRGKLAPRER PPSGTLEKLV SEAKAQLRDV QDFWISLPGT LCSEKMALST ASDDRCWNGM A; Positive Samples: NIH/3T3, Mouse brain, Rat brain; Cellular Location: Cell membrane, Endosome, Extracellular side, GPI-anchor, Lipid-anchor, extracellular space
Target Information
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: glypican proteoglycan 1; Glypican-1; GPI-anchored cell surface heparan sulfate proteoglycan; HSPG M12
Gene Aliases: AI462976; glypican; GPC1; HSPG M12; HSPGM12
UniProt ID: (Human) P35052, (Mouse) Q9QZF2, (Rat) P35053
Entrez Gene ID: (Human) 2817, (Mouse) 14733, (Rat) 58920
Schwann cell differentiation
heparan sulfate proteoglycan catabolic process
myelin assembly
negative regulation of fibroblast growth factor receptor signaling pathway
retinoid metabolic process
glycosaminoglycan biosynthetic process
glycosaminoglycan catabolic process
axon guidance
glycosaminoglycan metabolic process
positive regulation of skeletal muscle cell differentiation
biological_process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support