Proteintech

Glypican 4 Polyclonal Antibody

Glypican 4 Antibody in Western Blot (WB)

FIGURE: 1 / 3

Glypican 4 Antibody (13048-1-AP) in WB

Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13048-1-AP (Glypican 4 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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Product Details

13048-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Glypican 4 Fusion Protein Ag3670 (200-533 aa encoded by BC017166)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: FGDVPRKLK LQVTRAFVAA RTFAQGLAVA GDVVSKVSVV NPTAQCTHAL LKMIYCSHCR GLVTVKPCYN YCSNIMRGCL ANQGDLDFEW NNFIDAMLMV AERLEGPFNI ESVMDPIDVK ISDAIMNMQD NSVQVSQKVF QGCGPPKPLP AGRISRSISE SAFSARFRPH HPEERPTTAA GTSLDRLVTD VKEKLKQAKK FWSSLPSNVC NDERMAAGNG NEDDCWNGKG KSRYLFAVTG NGLANQGNNP EVQVDTSKPD ILILRQIMAL RVMTSKMKNA YNGNDVDFFD ISDESSGEGS GSGCEYQQCP SEFDYNATDH AGKSANEKAD SAGVR (200-533 aa encoded by BC017166)

Target Information

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The GPC4 gene is adjacent to the 3' end of GPC3 and may also play a role in Simpson-Golabi-Behmel syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Investigation of Glypican-4 and -6 by Infrared Spectral Imaging during the Hair Growth Cycle.

International journal of molecular sciences

Colin-Pierre C,Untereiner V,Sockalingum GD,Ramont L,Brézillon S

Tue Feb 21 00:00:00 UTC 2023

Cocaine preference and neuroadaptations are maintained by astrocytic NMDA receptors in the nucleus accumbens.

Science advances

Shelkar GP,Gandhi PJ,Liu J,Dravid SM

Fri Jul 22 00:00:00 UTC 2022

Transient astrocytic mGluR5 expression drives synaptic plasticity and subsequent chronic pain in mice.

The Journal of experimental medicine

Danjo Y,Shigetomi E,Hirayama YJ,Kobayashi K,Ishikawa T,Fukazawa Y,Shibata K,Takanashi K,Parajuli B,Shinozaki Y,Kim SK,Nabekura J,Koizumi S

Mon Apr 04 00:00:00 UTC 2022

Transient astrocytic mGluR5 expression drives synaptic plasticity and subsequent chronic pain in mice.

The Journal of experimental medicine

Danjo Y,Shigetomi E,Hirayama YJ,Kobayashi K,Ishikawa T,Fukazawa Y,Shibata K,Takanashi K,Parajuli B,Shinozaki Y,Kim SK,Nabekura J,Koizumi S

Mon Apr 04 00:00:00 UTC 2022

Synaptic hyperexcitability of cytomegalic pyramidal neurons contributes to epileptogenesis in tuberous sclerosis complex.

Cell reports

Wu X,Sosunov AA,Lado W,Teoh JJ,Ham A,Li H,Al-Dalahmah O,Gill BJA,Arancio O,Schevon CA,Frankel WN,McKhann GM,Sulzer D,Goldman JE,Tang G

Tue Jul 19 00:00:00 UTC 2022

Neuronal Glypican4 promotes mossy fiber sprouting through the mTOR pathway after pilocarpine-induced status epilepticus in mice.

Experimental neurology

Ma KG,Hu HB,Zhou JS,Ji C,Yan QS,Peng SM,Ren LD,Yang BN,Xiao XL,Ma YB,Wu F,Si KW,Wu XL,Liu JX

Sat Jan 01 00:00:00 UTC 2022

Activity-dependent modulation of synapse-regulating genes in astrocytes.

eLife

Farhy-Tselnicker I,Boisvert MM,Liu H,Dowling C,Erikson GA,Blanco-Suarez E,Farhy C,Shokhirev MN,Ecker JR,Allen NJ

Wed Sep 08 00:00:00 UTC 2021

LRRTMs Organize Synapses through Differential Engagement of Neurexin and PTPσ.

Neuron

Roppongi RT,Dhume SH,Padmanabhan N,Silwal P,Zahra N,Karimi B,Bomkamp C,Patil CS,Champagne-Jorgensen K,Twilley RE,Zhang P,Jackson MF,Siddiqui TJ

Wed Apr 08 00:00:00 UTC 2020

CD36 inhibits β-catenin/c-myc-mediated glycolysis through ubiquitination of GPC4 to repress colorectal tumorigenesis.

Nature communications

Fang Y,Shen ZY,Zhan YZ,Feng XC,Chen KL,Li YS,Deng HJ,Pan SM,Wu DH,Ding Y

Wed Sep 04 00:00:00 UTC 2019

CD36 inhibits β-catenin/c-myc-mediated glycolysis through ubiquitination of GPC4 to repress colorectal tumorigenesis.

Nature communications

Fang Y,Shen ZY,Zhan YZ,Feng XC,Chen KL,Li YS,Deng HJ,Pan SM,Wu DH,Ding Y

Wed Sep 04 00:00:00 UTC 2019

Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.

Nature communications

Lu IL,Chen C,Tung CY,Chen HH,Pan JP,Chang CH,Cheng JS,Chen YA,Wang CH,Huang CW,Kang YN,Chang HY,Li LL,Chang KP,Shih YH,Lin CH,Kwan SY,Tsai JW

Wed Jun 27 00:00:00 UTC 2018

Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.

Nature communications

Lu IL,Chen C,Tung CY,Chen HH,Pan JP,Chang CH,Cheng JS,Chen YA,Wang CH,Huang CW,Kang YN,Chang HY,Li LL,Chang KP,Shih YH,Lin CH,Kwan SY,Tsai JW

Wed Jun 27 00:00:00 UTC 2018

Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.

Nature communications

Lu IL,Chen C,Tung CY,Chen HH,Pan JP,Chang CH,Cheng JS,Chen YA,Wang CH,Huang CW,Kang YN,Chang HY,Li LL,Chang KP,Shih YH,Lin CH,Kwan SY,Tsai JW

Wed Jun 27 00:00:00 UTC 2018

Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.

Nature communications

Lu IL,Chen C,Tung CY,Chen HH,Pan JP,Chang CH,Cheng JS,Chen YA,Wang CH,Huang CW,Kang YN,Chang HY,Li LL,Chang KP,Shih YH,Lin CH,Kwan SY,Tsai JW

Wed Jun 27 00:00:00 UTC 2018

Astrocyte-Secreted Glypican 4 Regulates Release of Neuronal Pentraxin 1 from Axons to Induce Functional Synapse Formation.

Neuron

Farhy-Tselnicker I,van Casteren ACM,Lee A,Chang VT,Aricescu AR,Allen NJ

Wed Oct 11 00:00:00 UTC 2017

Astrocyte-Secreted Glypican 4 Regulates Release of Neuronal Pentraxin 1 from Axons to Induce Functional Synapse Formation.

Neuron

Farhy-Tselnicker I,van Casteren ACM,Lee A,Chang VT,Aricescu AR,Allen NJ

Wed Oct 11 00:00:00 UTC 2017

Expression of Glypican-4 in the brains of epileptic patients and epileptic animals and its effects on epileptic seizures.

Biochemical and biophysical research communications

Xiong Y,Zhang Y,Zheng F,Yang Y,Xu X,Wang W,Zhu B,Wang X

Fri Sep 09 00:00:00 UTC 2016

Expression of Glypican-4 in the brains of epileptic patients and epileptic animals and its effects on epileptic seizures.

Biochemical and biophysical research communications

Xiong Y,Zhang Y,Zheng F,Yang Y,Xu X,Wang W,Zhu B,Wang X

Fri Sep 09 00:00:00 UTC 2016

Expression of Glypican-4 in the brains of epileptic patients and epileptic animals and its effects on epileptic seizures.

Biochemical and biophysical research communications

Xiong Y,Zhang Y,Zheng F,Yang Y,Xu X,Wang W,Zhu B,Wang X

Fri Sep 09 00:00:00 UTC 2016

Expression of Glypican-4 in the brains of epileptic patients and epileptic animals and its effects on epileptic seizures.

Biochemical and biophysical research communications

Xiong Y,Zhang Y,Zheng F,Yang Y,Xu X,Wang W,Zhu B,Wang X

Fri Sep 09 00:00:00 UTC 2016

Astrocyte glypicans 4 and 6 promote formation of excitatory synapses via GluA1 AMPA receptors.

Nature

Allen NJ,Bennett ML,Foo LC,Wang GX,Chakraborty C,Smith SJ,Barres BA

Sun May 27 00:00:00 UTC 2012

Astrocyte glypicans 4 and 6 promote formation of excitatory synapses via GluA1 AMPA receptors.

Nature

Allen NJ,Bennett ML,Foo LC,Wang GX,Chakraborty C,Smith SJ,Barres BA

Sun May 27 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: dJ900E8.1 (glypican 4); glypican proteoglycan 4; Glypican-4; K-glypican

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Gene Aliases: 9530073D23Rik; AF311610; AI385680; AI661372; GPC4; K-glypican; UNQ474/PRO937

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UniProt ID: (Human) O75487, (Mouse) P51655

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Entrez Gene ID: (Human) 2239, (Rat) 317322, (Mouse) 14735

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Function(s)

heparan sulfate proteoglycan binding coreceptor activity involved in Wnt signaling pathway, planar cell polarity pathway molecular_function

Process(es)

biological_process retinoid metabolic process glycosaminoglycan biosynthetic process glycosaminoglycan catabolic process cell proliferation anatomical structure morphogenesis glycosaminoglycan metabolic process Wnt signaling pathway, planar cell polarity pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Glypican 4 Polyclonal Antibody

Glypican 4 Antibody (13048-1-AP) in WB

Product Details

13048-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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