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Invitrogen
Glypican 4 Polyclonal Antibody
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Glypican 4 Antibody (PA5-88766) in WB
Western blot analysis of Glypican 4 in extracts of various cell lines. Samples were incubated with Glypican 4 Polyclonal antibody (Product # PA5-88766) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-88766
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 357-556 of human GPC4 (NP_0014392)
Conjugate
Form
Liquid
Concentration
0.63 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2805115
Product Specific Information
Immunogen sequence: SESAFSARFR PHHPEERPTT AAGTSLDRLV TDVKEKLKQA KKFWSSLPSN VCNDERMAAG NGNEDDCWNG KGKSRYLFAV TGNGLANQGN NPEVQVDTSK PDILILRQIM ALRVMTSKMK NAYNGNDVDF FDISDESSGE GSGSGCEYQQ CPSEFDYNAT DHAGKSANEK ADSAGVRPGA QAYLLTVFCI LFLVMQREWR; Positive Samples: U-87MG, A-431, 293T; Cellular Location: Cell membrane, Extracellular side, GPI-anchor, Lipid-anchor, extracellular space
Target Information
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The GPC4 gene is adjacent to the 3' end of GPC3 and may also play a role in Simpson-Golabi-Behmel syndrome.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: dJ900E8.1 (glypican 4); glypican proteoglycan 4; Glypican-4; K-glypican
Gene Aliases: 9530073D23Rik; AF311610; AI385680; AI661372; GPC4; K-glypican; UNQ474/PRO937
UniProt ID: (Human) O75487, (Mouse) P51655
Entrez Gene ID: (Human) 2239, (Mouse) 14735
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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