Invitrogen

Gnb1 Polyclonal Antibody

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Gnb1 Antibody (PA1-725) in WB

Western blot analysis was performed on whole cell extracts (30 µg lysate) of MCF7 (Lane 1), Jurkat (Lane 2), MADA-MB-231 (Lane 3), T-47D (Lane 4) and tissue extract of Rat Brain (Lane 5). The blot was probed with Anti-Gnb1 Polyclonal Antibody (Product # PA1-725, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4,000 dilution). A 37 kDa band corresponding to Gnb1 was observed across cell lines and tissue tested. Along with the ... View More

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Gnb1 Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein in Gnb1 knockout (KO) cell line compared to control cell line using anti-Gnb1 Polyclonal Antibody (Product # PA1-725). {KO}

Product Details

PA1-725

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 6 publications 6 publications

Product Specifications

Species Reactivity

Bovine, Fruit fly, Human, Mouse, Sheep, Rat

Published species

Bovine, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: R(8) Q E A E Q L K N Q I R D A R K A C(25)

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_325605

Product Specific Information

PA1-725 detects transducin beta (T beta) from sheep, bovine, mouse and drosophila.

PA1-725 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~37 kDa protein representing Tr beta from sheep and bovine retinal and optic nerve extracts as well as mouse eye samples and drosophila.

The PA1-725 immunizing peptide corresponds to amino acid residues 8-25 from human Tr beta. This sequence is completely conserved between human, amphibian, bovine and rat. PA1-725 immunizing peptide (Cat. # PEP-154) is available for use in neutralization and control experiments.

Target Information

Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light into an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentration causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin activity is believed to be shut off by phosphorylation followed by binding of the soluble protein, arrestin. Transducin, once activated by rhodopsin, promotes the hydrolysis of cGMP by PDE. The subunit composition of transducin differs between different photoreceptor cells. Rod transducin consists of rod transducin alpha (Tr alpha), T beta, and T gamma. Cone transducin is composed of cone transducin alpha (Tc alpha), T beta and T gamma. Differential transducin subunit composition of transducin is believed to be responsible for the different light sensitivities between photoreceptive cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Phosphorylation of phosducin accelerates rod recovery from transducin translocation.

Investigative ophthalmology & visual science

Belcastro M,Song H,Sinha S,Song C,Mathers PH,Sokolov M

PA1-725 was used in western blot to study the ability of phosducin phosphorylation to markedly accelerate the recovery of rods from transducin translocation following exposure to rod-saturating light

Tue May 01 00:00:00 UTC 2012

RAS-converting enzyme 1-mediated endoproteolysis is required for trafficking of rod phosphodiesterase 6 to photoreceptor outer segments.

Proceedings of the National Academy of Sciences of the United States of America

Christiansen JR,Kolandaivelu S,Bergo MO,Ramamurthy V

PA1-725 was used in western blot to investigate the role of RAS-converting enzyme 1 RCE1 in intracellular PDE6 transport and photoreceptor viability

Tue May 24 00:00:00 UTC 2011

Mechanism for the regulation of mammalian cGMP phosphodiesterase6. 1: identification of its inhibitory subunit complexes and their roles.

Molecular and cellular biochemistry

Yamazaki A,Bondarenko VA,Matsuura I,Tatsumi M,Kurono S,Komori N,Matsumoto H,Hayashi F,Yamazaki RK,Usukura J

PA1-725 was used in western blot to study the regulatory mechanism for mammalian cGMP phosphodiesterase 6 actvity

Tue Jun 01 00:00:00 UTC 2010

Phosducin regulates the expression of transducin betagamma subunits in rod photoreceptors and does not contribute to phototransduction adaptation.

The Journal of general physiology

Krispel CM,Sokolov M,Chen YM,Song H,Herrmann R,Arshavsky VY,Burns ME

PA1-725 was used in western blot to investigate the role of phosducin during the expression of transducin beta/gamma subunits and phototransduction adaptation in rod photoreceptors

Sat Sep 01 00:00:00 UTC 2007

Disruption of the gene encoding the beta1-subunit of transducin in the Rd4/+ mouse.

Investigative ophthalmology & visual science

Kitamura E,Danciger M,Yamashita C,Rao NP,Nusinowitz S,Chang B,Farber DB

PA1-725 was used in western blot to investigate the role of Gnb1 for Rd4 retinal disease

Sat Apr 01 00:00:00 UTC 2006

Phototransduction in transgenic mice after targeted deletion of the rod transducin alpha -subunit.

Proceedings of the National Academy of Sciences of the United States of America

Calvert PD,Krasnoperova NV,Lyubarsky AL,Isayama T,Nicoló M,Kosaras B,Wong G,Gannon KS,Margolskee RF,Sidman RL,Pugh EN,Makino CL,Lem J

PA1-725 was used in western blot to investigate transducin subunits' functional specificity in phototransduction

Tue Dec 05 00:00:00 UTC 2000

Bioinformatics

Protein Aliases: beta subunit, signal-transducing proteins GS/GI; gbb1; guanine nucleotide binding protein (G protein), beta 1; guanine nucleotide binding protein (G protein), beta polypeptide 1; guanine nucleotide binding protein, beta 1; Guanine nucleotide-binding protein beta 1; Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1; guanine nucleotide-binding protein, beta-1 subunit; T beta; testicular tissue protein Li 72; Transducin beta chain 1

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Gene Aliases: AA409223; BOS_15860; C77571; Gnb-1; GNB1; MRD42

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UniProt ID: (Bovine) P62871, (Human) P62873, (Mouse) P62874, (Rat) P54311

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Entrez Gene ID: (Bovine) 281201, (Sheep) 101104166, (Human) 2782, (Mouse) 14688, (Rat) 24400

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Function(s)

GTPase activity signal transducer activity GTPase binding protein binding spectrin binding type 1 angiotensin receptor binding protein complex binding G-protein coupled receptor binding

Process(es)

signal transduction G-protein coupled receptor signaling pathway phospholipase C-activating G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration phototransduction, visible light cell proliferation cardiac muscle cell apoptotic process sensory perception of taste retina development in camera-type eye cellular response to hypoxia adenylate cyclase-activating dopamine receptor signaling pathway cellular response to prostaglandin E stimulus cellular response to catecholamine stimulus protein folding G-protein coupled acetylcholine receptor signaling pathway Wnt signaling pathway, calcium modulating pathway Ras protein signal transduction rhodopsin mediated signaling pathway platelet activation cellular response to glucagon stimulus metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Gnb1 Polyclonal Antibody

Gnb1 Antibody (PA1-725) in WB

Product Details

PA1-725

Western Blot (WB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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