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Invitrogen
Goat anti-Human IgG Fc Cross-Adsorbed Secondary Antibody, DyLight™ 488
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FIGURE: 1 / 3
Human IgG Fc Cross-Adsorbed Secondary Antibody (SA5-10134) in ICC/IF
Immunofluorescence analysis of Goat anti-Human IgG Fc Cross-Adsorbed, DyLight™ 488 was performedusing log phase ARH-77 cells (IgG producing B-cell line). The cells were fixed with 4% paraformaldehyde for 10 minutes,permeabilized with 0.1% Triton™ X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cellswere labeled with Goat anti-Human IgG Fc Cross-Adsorbed, DyLight™ 488 (Produc... View More
Product Details
SA5-10134
Applications
Tested Dilution
Western Blot (WB)
1:5,000-1:20,000
Immunohistochemistry (IHC)
1:50-1:500
Immunocytochemistry (ICC/IF)
1:50-1:500
Flow Cytometry (Flow)
1:50- 1:200
Immunoprecipitation (IP)
Assay-dependent
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Human IgG-Fc Fragment
Conjugate
Excitation/Emission Max
492/519 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 6.8 to 7.4, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2556714
Target
IgG Fc
Cross Adsorption
Against bovine, chicken, horse, mouse, rabbit, rat and sheep
Antibody Form
Whole Antibody
Product Specific Information
This antibody is cross-adsorbed and exhibits minimum reactivity to bovine, chicken, horse, mouse, rabbit, rat and sheep.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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