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Invitrogen
F(ab')2-Goat anti-Rat IgG (H+L) Secondary Antibody, FITC, eBioscience™
FIGURE: 1 / 1
Rat IgG (H+L) Secondary Antibody (11-4017-82) in Flow
Staining of BALB/c splenocytes with Rat IgG2a K Isotype Control Purified (Product # 14-4321-82) (blue histogram) or Anti-Mouse CD4 Purified (clone RM4-5, a rat IgG2a isotype, Product # 14-0042-82) (left) or Anti-Mouse CD8a Purified (clone 53-6-7, a rat IgG2a isotype, Product # 14-0081-82) (right) followed by 0.125 µg of F (ab')2 Anti-Rat IgG FITC (purple histogram). Cells in the lymphocyte gate were used fo... View More
Product Details
11-4017-82
Applications
Tested Dilution
Flow Cytometry (Flow)
0.25 µg/test
Product Specifications
Species Reactivity
Rat
Host/Isotype
Goat
/ Ig
Class
Polyclonal
Type
Secondary Antibody
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2572491
Target
IgG
Antibody Form
F(ab')2 Fragment
Product Specific Information
Description: The goat anti-rat IgG polyclonal antibody reacts with the heavy and light chains of rat IgG, with minimal cross reactivity to human, mouse, bovine, goat, hamster, and rabbit serum proteins. Reactivity has been shown to all IgG subclasses: IgG1, IgG2a, IgG2b, IgG2c, and some crossreactivity to rat IgM. The antibody is a F(ab')2 fragment which contains the 2 Fab domains still linked through the hinge region of the immunoglobulin. Removal of the Fc region helps to reduce issues typically found with Fc-mediated binding.
This polyclonal has been validated as a secondary reagent (its ability to recognize cells stained with a rat IgG followed by this polyclonal antibody).
Applications Reported: This poly antibody has been reported for use in flow cytometric analysis.
Applications Tested: This poly antibody has been tested by flow cytometric analysis of mouse splenocytes stained with a primary antibody of the rat IgG isotype. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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