Invitrogen

Goat anti-Syrian Hamster IgG (H+L) Secondary Antibody, FITC, eBioscience™

Syrian Hamster IgG (H+L) Secondary Antibody in Flow Cytometry (Flow)

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Syrian Hamster IgG (H+L) Secondary Antibody (11-4211-85) in Flow

Staining of BALB/c splenocytes with Anti-Mouse CD19 APC (Product # 17-0193-82) and 0.25 µg of Golden Syrian hamster IgG Isotype Control Functional Grade Purified (Product # 16-4914-85) (left) or 0.25 µg of Anti-Mouse CD3e Functional Grade Purified (right) followed by Anti-Syrian Hamster IgG FITC (Product # 11-4211-85).Cells in the lymphocyte gate were used for analysis.

Syrian Hamster IgG (H+L) Secondary Antibody in Western Blot (WB)

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Product Details

11-4211-85

Applications

Tested Dilution

Western Blot (WB)

1:3,000-1:5,000

Flow Cytometry (Flow)

0.5 µg/test

Product Specifications

Species Reactivity

Hamster

Host/Isotype

Goat / Ig

Class

Polyclonal

Type

Secondary Antibody

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_465223

Target

IgG

Antibody Form

Whole Antibody

Product Specific Information

Description: This antibody reacts with the heavy chains on Syrian hamster IgG and with light chains common to most Syrian hamster immunoglobulins. Reactions to non-immunoglobulin hamster proteins have not been detected.

Applications Reported: This polyclonal antibody can be used for detection of purified Syrian hamster immunoglobulins (IgG) in flow cytometry. At high concentrations, cross-reaction to Armenian hamster IgG has been observed.

Applications Tested: FITC Anti-Syrian Hamster IgG has been tested by flow cytometric analysis to detect purified Syrian hamster monoclonal antibodies. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. In addition, it has been adsorbed and tested by ELISA to ensure minimal cross reaction to human, bovine, horse, rabbit, rat, and mouse serum proteins, but may cross-react with immunoglobµLins from other species.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Anti-Hamster secondary antibodies are affinity-purified antibodies with well-characterized specificity for hamster immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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