Invitrogen

Granzyme B Monoclonal Antibody (16G6), eBioscience™

Granzyme B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Granzyme B Antibody (14-8822-82) in IHC (P)

Immunohistochemical analysis of Granzyme B was performed using formalin-fixed paraffin-embedded human tonsil tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Product # 00-4956-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius for 15 minutes... View More

Granzyme B Antibody in Western Blot (WB)

Granzyme B Antibody in Immunohistochemistry (IHC)

Granzyme B Antibody in Flow Cytometry (Flow)

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Product Details

14-8822-82

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 4 publications 4 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-Dependent

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Flow Cytometry (Flow)

View 14 publications 14 publications

ELISA (ELISA)

1-4 µg/mL

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

16G6

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_468530

Product Specific Information

Description: The 16G6 antibody reacts with mouse Granzyme B (GrB) which is a member of the granzyme serine protease family. GrB is found in the granules of cytotoxic T cells and NK cells. Granzyme B has also been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation. GrB has been called CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. GrB is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved has been identified as mannose 6-phosphate receptor. This receptor functions as a death receptor for granzyme B during cytotoxic T cell-induced apoptosis.

For intracellular staining and flow cytometric analysis with direct conjugates of anti-mouse Granzyme B, it is highly recommended to use the Foxp3 Staining Buffer Set (Product # 00-5523). Other buffers may yield varying results.

Applications Reported: This 16G6 antibody has been reported for use in western blotting, immunohistochemical staining, ELISA capture antibody, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

Applications Tested: The unlabelled 16G6 antibody has been tested as the capture antibody in a sandwich ELISA for quantitation of mouse granzyme B protein levels, in combination with the biotinylated eBioLUEE (Product # 13-8821-81) antibody for detection and recombinant mouse granzyme B as the standard. A suitable range of concentrations of this antibody for ELISA detection is 1-4 µg/mL.

The 16G6 antibody has been tested for Western blotting; a recommended starting concentration is 1 µg/mL. The 16G6 antibody has been used for formalin-fixed paraffin embedded tissue using low pH antigen retrieval at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Granzyme B is a member of the granzyme serine protease family, and is found in the granules of cytotoxic T cells and NK cells. Granzyme B has been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation, and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. Granzyme B is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved in this process has been identified as mannose 6-phosphate receptor which functions as a death receptor for Granzyme B during cytotoxic T cell-induced apoptosis. Granzyme B enters target cells to cleave caspase-3 and initiate the caspase cascade leading to DNA fragmentation and apoptosis. Granzyme B can also act through a mitochondrial apoptosis pathway by cleaving the Bid protein. Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes (A to G) have been isolated and cloned from mouse CTLs and NK cells, however in man, fewer have been cloned and identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The Sesquiterpene Lactone-Rich Fraction of Inula helenium L. Enhances the Antitumor Effect of Anti-PD-1 Antibody in Colorectal Cancer: Integrative Phytochemical, Transcriptomic, and Experimental Analyses.

Cancers

Chun J,Park SM,Lee M,Ha IJ,Jeong MK

Published figure using Granzyme B monoclonal antibody (Product # 14-8822-82) in Immunohistochemistry

Fri Jan 20 00:00:00 UTC 2023

Radiofrequency ablation in combination with CD73 inhibitor AB680 reduces tumor growth and enhances anti-tumor immunity in a syngeneic model of pancreatic ductal adenocarcinoma.

Frontiers in oncology

Faraoni EY,Strickland LN,O'Brien BJ,Barraza JF,Thosani NC,Wray CJ,Mills TW,Bailey-Lundberg JM

Published figure using Granzyme B monoclonal antibody (Product # 14-8822-82) in Immunohistochemistry

Wed Sep 04 00:00:00 UTC 2024

Direct Tumor Killing and Immunotherapy through Anti-SerpinB9 Therapy.

Cell

Jiang L,Wang YJ,Zhao J,Uehara M,Hou Q,Kasinath V,Ichimura T,Banouni N,Dai L,Li X,Greiner DL,Shultz LD,Zhang X,Sun ZJ,Curtin I,Vangos NE,Yeoh ZC,Geffken EA,Seo HS,Liu ZX,Heffron GJ,Shah K,Dhe-Paganon S,Abdi R

14-8822-82 was used in Western Blot, Immunohistochemistry (Frozen) to describe a molecular target that permits a combination of tumor ablation, interference within the TME, and immunotherapy in one potential modality.

Wed Nov 25 00:00:00 UTC 2020

Direct Tumor Killing and Immunotherapy through Anti-SerpinB9 Therapy.

Cell

Wed Nov 25 00:00:00 UTC 2020

Regulation of the effector function of CD8⁺ T cells by gut microbiota-derived metabolite butyrate.

Scientific reports

Luu M,Weigand K,Wedi F,Breidenbend C,Leister H,Pautz S,Adhikary T,Visekruna A

14-8822 was used in Flow cytometry/Cell sorting to might have significant implications in adoptive immunotherapy of cancers and in anti-viral immunity.

Wed Sep 26 00:00:00 UTC 2018

PD-1 Blockade Prevents the Development and Progression of Carcinogen-Induced Oral Premalignant Lesions.

Cancer prevention research (Philadelphia, Pa.)

Wang J,Xie T,Wang B,William WN,Heymach JV,El-Naggar AK,Myers JN,Caulin C

Published figure using Granzyme B monoclonal antibody (Product # 14-8822-82) in Immunohistochemistry

Fri Dec 01 00:00:00 UTC 2017

Imaging Granzyme B Activity Assesses Immune-Mediated Myocarditis.

Circulation research

Konishi M,Erdem SS,Weissleder R,Lichtman AH,McCarthy JR,Libby P

Published figure using Granzyme B monoclonal antibody (Product # 14-8822-82) in Western Blot

Fri Aug 28 00:00:00 UTC 2015

The granzyme B-Serpinb9 axis controls the fate of lymphocytes after lysosomal stress.

Cell death and differentiation

Bird CH,Christensen ME,Mangan MS,Prakash MD,Sedelies KA,Smyth MJ,Harper I,Waterhouse NJ,Bird PI

14-8822 was used in Western Blotting to demonstrate that restimulation of previously activated human/mouse lymphocytes induces lysosomal membrane permeabilisation, followed by granzyme B release from lysosome-related organelles into the cytotoxic lymphocyte cytosol.

Sun Jun 01 00:00:00 UTC 2014

A natural genetic variant of granzyme B confers lethality to a common viral infection.

PLoS pathogens

Andoniou CE,Sutton VR,Wikstrom ME,Fleming P,Thia KY,Matthews AY,Kaiserman D,Schuster IS,Coudert JD,Eldi P,Chaudhri G,Karupiah G,Bird PI,Trapani JA,Degli-Esposti MA

14-8822 was used in Western Blotting to describe the biochemical and cytotoxic functions of a common allele of granzyme B.

Mon Dec 01 00:00:00 UTC 2014

IL-2 induction of Blimp-1 is a key in vivo signal for CD8+ short-lived effector T cell differentiation.

Journal of immunology (Baltimore, Md. : 1950)

Boulet S,Daudelin JF,Labrecque N

14-8822 was used in Flow cytometry/Cell sorting to demonstrate that IL-2 induction of Blimp-1 expression is a key regulator of short-lived effector cell differentiation.

Fri Aug 15 00:00:00 UTC 2014

Imiquimod clears tumors in mice independent of adaptive immunity by converting pDCs into tumor-killing effector cells.

The Journal of clinical investigation

Drobits B,Holcmann M,Amberg N,Swiecki M,Grundtner R,Hammer M,Colonna M,Sibilia M

14-8822 was used in Flow cytometry/Cell sorting to demonstrate that imiquimod treatment causes CCL2-dependent recruitment of pDCs and their transformation into killer DCs, enabling elimination of tumour cells.

Wed Feb 01 00:00:00 UTC 2012

Coreceptor gene imprinting governs thymocyte lineage fate.

The EMBO journal

Adoro S,McCaughtry T,Erman B,Alag A,Van Laethem F,Park JH,Tai X,Kimura M,Wang L,Grinberg A,Kubo M,Bosselut R,Love P,Singer A

14-8822 was used in Flow cytometry/Cell sorting to identify coreceptor gene imprinting as a critical determinant of lineage fate determination in the thymus.

Wed Jan 18 00:00:00 UTC 2012

Type-I IFN drives the differentiation of short-lived effector CD8+ T cells in vivo.

European journal of immunology

Wiesel M,Crouse J,Bedenikovic G,Sutherland A,Joller N,Oxenius A

14-8822 was used in Flow cytometry/Cell sorting to study in vivo the role of type-I interferon in the differentiation of CD8+ T cells.

Wed Feb 01 00:00:00 UTC 2012

Serological identification of HSP105 as a novel non-Hodgkin lymphoma therapeutic target.

Blood

Zappasodi R,Bongarzone I,Ghedini GC,Castagnoli L,Cabras AD,Messina A,Tortoreto M,Tripodo C,Magni M,Carlo-Stella C,Gianni AM,Pupa SM,Di Nicola M

14-8822 was used in in vivo experiments to identify HSP105 as a nononcogene that can be exploited as an antilymphoma target.

Thu Oct 20 00:00:00 UTC 2011

A LysM and SH3-domain containing region of the Listeria monocytogenes p60 protein stimulates accessory cells to promote activation of host NK cells.

PLoS pathogens

Schmidt RL,Filak HC,Lemon JD,Potter TA,Lenz LL

14-8822 was used in Flow cytometry/Cell sorting to demonstrate an immune stimulating function for a bacterial LysM domain-containing polypeptide.

Tue Nov 01 00:00:00 UTC 2011

Rescue of tumor-infiltrating lymphocytes from activation-induced cell death enhances the antitumor CTL response in CD5-deficient mice.

Journal of immunology (Baltimore, Md. : 1950)

Tabbekh M,Franciszkiewicz K,Haouas H,Lécluse Y,Benihoud K,Raman C,Mami-Chouaib F

14-8822 was used in Flow cytometry/Cell sorting to compare the T cell response to the B16F10 melanoma engrafted into CD5-deficient and wild-type C57BL/6 mice.

Fri Jul 01 00:00:00 UTC 2011

Naive tumor-specific CD4(+) T cells differentiated in vivo eradicate established melanoma.

The Journal of experimental medicine

Xie Y,Akpinarli A,Maris C,Hipkiss EL,Lane M,Kwon EK,Muranski P,Restifo NP,Antony PA

14-8822 was used in Flow cytometry/Cell sorting to study the regression of tumours due to naive tumor-specific CD4(+) T cell differentiation.

Mon Mar 15 00:00:00 UTC 2010

IL-18, but not IL-15, contributes to the IL-12-dependent induction of NK-cell effector functions by Leishmania infantum in vivo.

European journal of immunology

Haeberlein S,Sebald H,Bogdan C,Schleicher U

14-8822 was used in Flow cytometry/Cell sorting to investigate whether IL-15 or IL-18 mediate the activity of IL-12, showing that IL-18 contributes to the IL-12-dependent induction of NK-cell effector functions by Leishmania infantum.

Tue Jun 01 00:00:00 UTC 2010

Store-operated Ca2+ entry through ORAI1 is critical for T cell-mediated autoimmunity and allograft rejection.

Journal of immunology (Baltimore, Md. : 1950)

McCarl CA,Khalil S,Ma J,Oh-hora M,Yamashita M,Roether J,Kawasaki T,Jairaman A,Sasaki Y,Prakriya M,Feske S

14-8822 was used in Flow cytometry/Cell sorting to reaffirm the critical role of ORAI1 for T cell function, and provide important insights into the in vivo functions of CRAC channels for T cell-mediated immunity.

Mon Nov 15 00:00:00 UTC 2010

IL-15 is critical for the maintenance and innate functions of self-specific CD8(+) T cells.

European journal of immunology

Itsumi M,Yoshikai Y,Yamada H

14-8822 was used in Flow cytometry/Cell sorting to study the role of memory phenotype CD8+ cells in host defence mechanisms and their origin and antigen specificity.

Wed Jul 01 00:00:00 UTC 2009

De novo recruitment of antigen-experienced and naive T cells contributes to the long-term maintenance of antiviral T cell populations in the persistently infected central nervous system.

Journal of immunology (Baltimore, Md. : 1950)

Zhao J,Zhao J,Perlman S

14-8822 was used in Flow cytometry/Cell sorting to establish the source of virus-specific CD4 and CD8 T cells at the site of hepatitis infection.

Thu Oct 15 00:00:00 UTC 2009

Enhanced efficacy and reduced toxicity of multifactorial adjuvants compared with unitary adjuvants as cancer vaccines.

Blood

Ahonen CL,Wasiuk A,Fuse S,Turk MJ,Ernstoff MS,Suriawinata AA,Gorham JD,Kedl RM,Usherwood EJ,Noelle RJ

14-8822 was used in Flow cytometry/Cell sorting to report the therapeutic effects of combinatorial use of CD40 and TLR agonists as a cancer vaccine.

Sat Mar 15 00:00:00 UTC 2008

Enhancement of CD8 T-cell function through modifying surface glycoproteins in young and old mice.

Immunology

Sadighi Akha AA,Berger SB,Miller RA

Published figure using Granzyme B monoclonal antibody (Product # 14-8822-82) in Flow Cytometry

Sun Oct 01 00:00:00 UTC 2006

Bioinformatics

Protein Aliases: C11; Cathepsin G-like 1; CCP1; CTLA-1; CTSGL1; Cytotoxic cell protease 1; cytotoxic serine protease B; Cytotoxic T lymphocyte associated serine esterase 1; Cytotoxic T-lymphocyte proteinase 2; cytotoxic T-lymphocyte-associated serine esterase 1; fragmentin 2; Fragmentin-2; granzyme 2; Granzyme B; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); Granzyme B(G,H); Granzyme-2; GranzymeB; HLP; Human lymphocyte protein; Human lymphocyte protein (Hlp); Lymphocyte protease; OTTHUMP00000028189; SECT; T-cell serine protease 1-3E

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Gene Aliases: AI553453; CCP-1/C11; CCP1; CCPI; CGL-1; CGL1; CSP-B; CSPB; Ctla-1; CTLA1; CTSGL1; GRB; GZB; GZMB; HLP; SECT

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UniProt ID: (Human) P10144, (Mouse) P04187

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Entrez Gene ID: (Human) 3002, (Mouse) 14939

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Function(s)

serine-type endopeptidase activity protein binding serine-type peptidase activity peptidase activity hydrolase activity

Process(es)

apoptotic process protein processing cytolysis natural killer cell mediated cytotoxicity positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway T cell mediated cytotoxicity proteolysis granzyme-mediated apoptotic signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Granzyme B Monoclonal Antibody (16G6), eBioscience™

Granzyme B Antibody (14-8822-82) in IHC (P)

Product Details

14-8822-82

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

ELISA (ELISA)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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