Invitrogen

Granzyme B Monoclonal Antibody (NGZB), PE-Cyanine7, eBioscience™

Granzyme B Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 42

Granzyme B Antibody (25-8898-82) in Flow

C57BL/6 mouse splenocytes were stimulated for 72 hours with CD3e and CD28 Monoclonal Antibodies, Functional Grade (Product # 16-0031-85) and (Product # 16-0281-85), followed by Protein Transport Inhibitor Cocktail (Product # 00-4980-03), for an additional 5 hours. Cells were then surface stained with CD8a Monoclonal Antibody, FITC (Product # 11-0081-82) followed by intracellular staining of 0.06 µg of Rat IgG2a kappa Isotype Control, PE-Cyanine7 (Product # 25-4321-82) (left) or 0.06 µg of Granzyme B Monoclonal Antibody, PE-Cyanine7 (right)... View More

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Product Details

25-8898-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 48 publications 48 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

NGZB

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10853339

Product Specific Information

Description: This NGZB monoclonal antibody reacts with mouse Granzyme B, which is a member of the granzyme serine protease family. Granzyme B is found in the granules of cytotoxic T cells and NK cells. Granzyme B has also been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation, and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. Granzyme B is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved has been identified as mannose 6-phosphate receptor. This receptor functions as a death receptor for Granzyme B during cytotoxic T cell-induced apoptosis. This NGZB monoclonal antibody does not cross-react to human Granzyme B nor is staining blocked with GB11, suggesting it recognizes a different epitope.

Applications Reported: This NGZB antibody has been reported for use in intracellular staining and flow cytometric analysis.

Applications Tested: This NGZB antibody has been tested by intracellular staining and flow cytometric analysis of mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to BestProtocols®: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Granzyme B is a member of the granzyme serine protease family, and is found in the granules of cytotoxic T cells and NK cells. Granzyme B has been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation, and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. Granzyme B is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved in this process has been identified as mannose 6-phosphate receptor which functions as a death receptor for Granzyme B during cytotoxic T cell-induced apoptosis. Granzyme B enters target cells to cleave caspase-3 and initiate the caspase cascade leading to DNA fragmentation and apoptosis. Granzyme B can also act through a mitochondrial apoptosis pathway by cleaving the Bid protein. Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes (A to G) have been isolated and cloned from mouse CTLs and NK cells, however in man, fewer have been cloned and identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy.

Nature communications

Xiao X,Shi J,He C,Bu X,Sun Y,Gao M,Xiang B,Xiong W,Dai P,Mao Q,Xing X,Yao Y,Yu H,Xu G,Li S,Ren Y,Chen B,Jiang C,Meng G,Lee YR,Wei W,Freeman GJ,Xie C,Zhang J

Published figure using Granzyme B monoclonal antibody (Product # 25-8898-82) in Flow Cytometry

Fri May 19 00:00:00 UTC 2023

Sphingomyelin-derived nanovesicles for the delivery of the IDO1 inhibitor epacadostat enhance metastatic and post-surgical melanoma immunotherapy.

Nature communications

Wang Z,Li W,Jiang Y,Tran TB,Cordova LE,Chung J,Kim M,Wondrak G,Erdrich J,Lu J

Published figure using Granzyme B monoclonal antibody (Product # 25-8898-82) in Flow Cytometry

Thu Nov 09 00:00:00 UTC 2023

Small-molecule PTPN2 Inhibitors Sensitize Resistant Melanoma to Anti-PD-1 Immunotherapy.

Cancer research communications

Zhu Z,Tang R,Huff S,Kummetha IR,Wang L,Li N,Rana TM

Published figure using Granzyme B monoclonal antibody (Product # 25-8898-82) in Flow Cytometry

Sun Jan 01 00:00:00 UTC 2023

Combined PARP and WEE1 inhibition triggers anti-tumor immune response in BRCA1/2 wildtype triple-negative breast cancer.

NPJ breast cancer

Teo ZL,O'Connor MJ,Versaci S,Clarke KA,Brown ER,Percy LW,Kuykhoven K,Mintoff CP,Savas P,Virassamy B,Luen SJ,Byrne A,Sant S,Lindeman GJ,Darcy PK,Loi S

25-8898-82 was used in Flow cytometry/Cell sorting to identify baseline tumour-infiltrating lymphocyte (TIL) levels as a potential predictive biomarker of response to PARPi, WEE1i and immunotherapies in BRCA1/2 wild-type TNBC.

Tue Aug 15 00:00:00 UTC 2023

Meningeal lymphatics regulate radiotherapy efficacy through modulating anti-tumor immunity.

Cell research

Zhou C,Ma L,Xu H,Huo Y,Luo J

25-8898-82 was used in Flow Cytometry to suggest an essential role of the MLV-CLN network in RT-triggered anti-tumor immunity, and highlight the potential of VEGF-C mRNA for brain tumor therapy.

Wed Jun 01 00:00:00 UTC 2022

Histone methyltransferase WHSC1 loss dampens MHC-I antigen presentation pathway to impair IFN-γ-stimulated antitumor immunity.

The Journal of clinical investigation

Ren J,Li N,Pei S,Lian Y,Li L,Peng Y,Liu Q,Guo J,Wang X,Han Y,Zhang G,Wang H,Li Y,Jiang J,Li Q,Tan M,Peng J,Hu G,Xiao Y,Li X,Lin M,Qin J

Published figure using Granzyme B monoclonal antibody (Product # 25-8898-82) in Flow Cytometry

Fri Apr 15 00:00:00 UTC 2022

Wnt5A Signaling Blocks Progression of Experimental Visceral Leishmaniasis.

Frontiers in immunology

Maity S,Chakraborty A,Mahata SK,Roy S,Das AK,Sen M

25-8898-82 was used in Flow Cytometry to indicate that while depletion of Wnt5A promotes susceptibility to visceral leishmaniasis, revamping Wnt5A signaling in the host is able to curb L. donovani infection irrespective of antimony sensitivity or resistance and mitigate the progression of disease.

Tue Mar 22 00:00:00 UTC 2022

Mobilizing phospholipids on tumor plasma membrane implicates phosphatidylserine externalization blockade for cancer immunotherapy.

Cell reports

Wang W,Wu S,Cen Z,Zhang Y,Chen Y,Huang Y,Cillo AR,Prokopec JS,Quarato G,Vignali DAA,Stewart-Ornstein J,Li S,Lu B,Gong YN

Published figure using Granzyme B monoclonal antibody (Product # 25-8898-82) in Flow Cytometry

Tue Nov 01 00:00:00 UTC 2022

RAD21 amplification epigenetically suppresses interferon signaling to promote immune evasion in ovarian cancer.

The Journal of clinical investigation

Deng P,Wang Z,Chen J,Liu S,Yao X,Liu S,Liu L,Yu Z,Huang Y,Xiong Z,Xiao R,Gao J,Liang W,Chen J,Liu H,Hong JH,Chan JY,Guan P,Chen J,Wang Y,Yin J,Li J,Zheng M,Zhang C,Zhou P,Kang T,Teh BT,Yu Q,Zuo Z,Jiang Q,Liu J,Xiong Y,Xia X,Tan J

Published figure using Granzyme B monoclonal antibody (Product # 25-8898-82) in Flow Cytometry

Tue Nov 15 00:00:00 UTC 2022

eEF2K promotes PD-L1 stabilization through inactivating GSK3β in melanoma.

Journal for immunotherapy of cancer

Chen X,Wang K,Jiang S,Sun H,Che X,Zhang M,He J,Wen Y,Liao M,Li X,Zhou X,Song J,Ren X,Yi W,Yang J,Chen X,Yin M,Cheng Y

Published figure using Granzyme B monoclonal antibody (Product # 25-8898-82) in Flow Cytometry

Tue Mar 01 00:00:00 UTC 2022

PRDM1/BLIMP1 induces cancer immune evasion by modulating the USP22-SPI1-PD-L1 axis in hepatocellular carcinoma cells.

Nature communications

Li Q,Zhang L,You W,Xu J,Dai J,Hua D,Zhang R,Yao F,Zhou S,Huang W,Dai Y,Zhang Y,Baheti T,Qian X,Pu L,Xu J,Xia Y,Zhang C,Tang J,Wang X

Published figure using Granzyme B monoclonal antibody (Product # 25-8898-82) in Flow Cytometry

Mon Dec 12 00:00:00 UTC 2022

Intranasal Delivery of Recombinant S100A8 Protein Delays Lung Cancer Growth by Remodeling the Lung Immune Microenvironment.

Frontiers in immunology

Wong SW,McCarroll J,Hsu K,Geczy CL,Tedla N

Published figure using Granzyme B monoclonal antibody (Product # 25-8898-82) in Flow Cytometry

Mon Jun 06 00:00:00 UTC 2022

Enteric viruses evoke broad host immune responses resembling those elicited by the bacterial microbiome.

Cell host & microbe

Dallari S,Heaney T,Rosas-Villegas A,Neil JA,Wong SY,Brown JJ,Urbanek K,Herrmann C,Depledge DP,Dermody TS,Cadwell K

25-8898-82 was used in Flow cytometry/Cell sorting to expand an understanding of the immune space occupied by the enteric virome and underscore the importance of viral exposure events.

Wed Jun 09 00:00:00 UTC 2021

Extracellular Vesicles from Akkermansia muciniphila Elicit Antitumor Immunity Against Prostate Cancer via Modulation of CD8⁺ T Cells and Macrophages.

International journal of nanomedicine

Luo ZW,Xia K,Liu YW,Liu JH,Rao SS,Hu XK,Chen CY,Xu R,Wang ZX,Xie H

Published figure using Granzyme B monoclonal antibody (Product # 25-8898-82) in Flow Cytometry

Tue Jun 01 00:00:00 UTC 2021

Blockade of 6-phosphogluconate dehydrogenase generates CD8⁺ effector T cells with enhanced anti-tumor function.

Cell reports

Daneshmandi S,Cassel T,Lin P,Higashi RM,Wulf GM,Boussiotis VA,Fan TW,Seth P

Published figure using Granzyme B monoclonal antibody (Product # 25-8898-82) in Flow Cytometry

Tue Mar 09 00:00:00 UTC 2021

Circadian Clock Disruption Suppresses PDL1⁺ Intraepithelial B Cells in Experimental Colitis and Colitis-Associated Colorectal Cancer.

Cellular and molecular gastroenterology and hepatology

Liu JL,Wang CY,Cheng TY,Rixiati Y,Ji C,Deng M,Yao S,Yuan LH,Zhao YY,Shen T,Li JM

Published figure using Granzyme B monoclonal antibody (Product # 25-8898-82) in Flow Cytometry

Mon Jan 24 00:00:00 UTC 2022

Integrin αvβ8 on T cells suppresses anti-tumor immunity in multiple models and is a promising target for tumor immunotherapy.

Cell reports

Dodagatta-Marri E,Ma HY,Liang B,Li J,Meyer DS,Chen SY,Sun KH,Ren X,Zivak B,Rosenblum MD,Headley MB,Pinzas L,Reed NI,Del Cid JS,Hann BC,Yang S,Giddabasappa A,Noorbehesht K,Yang B,Dal Porto J,Tsukui T,Niessen K,Atakilit A,Akhurst RJ,Sheppard D

Published figure using Granzyme B monoclonal antibody (Product # 25-8898-82) in Flow Cytometry

Tue Jul 06 00:00:00 UTC 2021

Non-redundant activity of GSK-3α and GSK-3β in T cell-mediated tumor rejection.

iScience

Steele L,Mannion AJ,Shaw G,Maclennan KA,Cook GP,Rudd CE,Taylor A

25-8898-82 was used in Flow Cytometry to demonstrate that both isoforms contribute to T cell function to different degrees.

Fri Jun 25 00:00:00 UTC 2021

Non-redundant activity of GSK-3α and GSK-3β in T cell-mediated tumor rejection.

iScience

Steele L,Mannion AJ,Shaw G,Maclennan KA,Cook GP,Rudd CE,Taylor A

25-8898-82 was used in Flow Cytometry to demonstrate that both isoforms contribute to T cell function to different degrees.

Fri Jun 25 00:00:00 UTC 2021

Aiduqing formula inhibits breast cancer metastasis by suppressing TAM/CXCL1-induced Treg differentiation and infiltration.

Cell communication and signaling : CCS

Li J,Wang S,Wang N,Zheng Y,Yang B,Wang X,Zhang J,Pan B,Wang Z

Published figure using Granzyme B monoclonal antibody (Product # 25-8898-82) in Flow Cytometry

Mon Aug 30 00:00:00 UTC 2021

Metabolic requirements of NK cells during the acute response against retroviral infection.

Nature communications

Littwitz-Salomon E,Moreira D,Frost JN,Choi C,Liou KT,Ahern DK,O'Shaughnessy S,Wagner B,Biron CA,Drakesmith H,Dittmer U,Finlay DK

25-8898-82 was used in Flow cytometry/Cell sorting to show that following infection natural killer cells increase nutrient uptake, including amino acids and iron, and reprogram their metabolic machinery by increasing glycolysis and mitochondrial metabolism.

Fri Sep 10 00:00:00 UTC 2021

Knockout of immunotherapy prognostic marker genes eliminates the effect of the anti-PD-1 treatment.

NPJ precision oncology

Yang N,Ji F,Cheng L,Lu J,Sun X,Lin X,Lan X

Published figure using Granzyme B monoclonal antibody (Product # 25-8898-82) in Flow Cytometry

Fri May 07 00:00:00 UTC 2021

Salmonella-Based Therapy Targeting Indoleamine 2,3-Dioxygenase Restructures the Immune Contexture to Improve Checkpoint Blockade Efficacy.

Biomedicines

Ebelt ND,Zuniga E,Marzagalli M,Zamloot V,Blazar BR,Salgia R,Manuel ER

Published figure using Granzyme B monoclonal antibody (Product # 25-8898-82) in Flow Cytometry

Wed Dec 16 00:00:00 UTC 2020

Cancer-Specific Loss of p53 Leads to a Modulation of Myeloid and T Cell Responses.

Cell reports

Blagih J,Zani F,Chakravarty P,Hennequart M,Pilley S,Hobor S,Hock AK,Walton JB,Morton JP,Gronroos E,Mason S,Yang M,McNeish I,Swanton C,Blyth K,Vousden KH

25-8898 was used in Flow cytometry/Cell sorting to show suppressive regulatory T cells accumulate in tumour cells in the absence of p53 indicating a pathway in which cancer cells circumvent immune destruction.

Tue Jan 14 00:00:00 UTC 2020

Cancer-Specific Loss of p53 Leads to a Modulation of Myeloid and T Cell Responses.

Cell reports

Blagih J,Zani F,Chakravarty P,Hennequart M,Pilley S,Hobor S,Hock AK,Walton JB,Morton JP,Gronroos E,Mason S,Yang M,McNeish I,Swanton C,Blyth K,Vousden KH

Tue Jan 14 00:00:00 UTC 2020

Assessment of the WAP-Myc mouse mammary tumor model for spontaneous metastasis.

Scientific reports

Utz B,Turpin R,Lampe J,Pouwels J,Klefström J

Published figure using Granzyme B monoclonal antibody (Product # 25-8898-82) in Flow Cytometry

Fri Oct 30 00:00:00 UTC 2020

Memory CD73+IgM+ B cells protect against Plasmodium yoelii infection and express Granzyme B.

PloS one

Parra M,Weitner M,Yang A,Akue A,Liu X,Schmidt T,Allman WR,Akkoyunlu M,Derrick SC

Published figure using Granzyme B monoclonal antibody (Product # 25-8898-82) in Flow Cytometry

Tue Oct 27 00:00:00 UTC 2020

GAPDH Overexpression in the T Cell Lineage Promotes Angioimmunoblastic T Cell Lymphoma through an NF-κB-Dependent Mechanism.

Cancer cell

Mondragón L,Mhaidly R,De Donatis GM,Tosolini M,Dao P,Martin AR,Pons C,Chiche J,Jacquin M,Imbert V,Proïcs E,Boyer L,Doye A,Luciano F,Neels JG,Coutant F,Fabien N,Sormani L,Rubio-Patiño C,Bossowski JP,Muller F,Marchetti S,Villa E,Peyron JF,Gaulard P,Lemonnier F,Asnafi V,Genestier L,Benhida R,Fournié JJ,Passeron T,Ricci JE,Verhoeyen E

25-8898-82 was used in Flow Cytometry to suggest the therapeutic potential of targeting NF-κB signaling in AITL and provide a model for future AITL therapeutic investigations.

Mon Sep 16 00:00:00 UTC 2019

Inflammation induced by incomplete radiofrequency ablation accelerates tumor progression and hinders PD-1 immunotherapy.

Nature communications

Shi L,Wang J,Ding N,Zhang Y,Zhu Y,Dong S,Wang X,Peng C,Zhou C,Zhou L,Li X,Shi H,Wu W,Long X,Wu C,Liao W

Published figure using Granzyme B monoclonal antibody (Product # 25-8898-82) in Flow Cytometry

Thu Nov 28 00:00:00 UTC 2019

Pharmacological reactivation of MYC-dependent apoptosis induces susceptibility to anti-PD-1 immunotherapy.

Nature communications

Haikala HM,Anttila JM,Marques E,Raatikainen T,Ilander M,Hakanen H,Ala-Hongisto H,Savelius M,Balboa D,Von Eyss B,Eskelinen V,Munne P,Nieminen AI,Otonkoski T,Schüler J,Laajala TD,Aittokallio T,Sihto H,Mattson J,Heikkilä P,Leidenius M,Joensuu H,Mustjoki S,Kovanen P,Eilers M,Leverson JD,Klefström J

25-8898-82 was used in Flow Cytometry to demonstrate that pharmacological reactivation of MYC-dependent apoptosis is a powerful antitumor strategy involving both tumor cell depletion and immunosurveillance.

Wed Feb 06 00:00:00 UTC 2019

Qa-1-Restricted CD8⁺ T Cells Can Compensate for the Absence of Conventional T Cells during Viral Infection.

Cell reports

Anderson CK,Reilly EC,Lee AY,Brossay L

25-8898 was used in Flow cytometry/Cell sorting to study compensatory mechanisms in immune responses to viruses when classical T cell immunity has been evaded.

Tue Apr 09 00:00:00 UTC 2019

The Transcription Factor Runx3 Establishes Chromatin Accessibility of cis-Regulatory Landscapes that Drive Memory Cytotoxic T Lymphocyte Formation.

Immunity

Wang D,Diao H,Getzler AJ,Rogal W,Frederick MA,Milner J,Yu B,Crotty S,Goldrath AW,Pipkin ME

25-8898 was used in Flow cytometry/Cell sorting to conclude that Runx3 governs chromatin accessibility during TCR stimulation and enforces the memory CTL developmental program.

Tue Apr 17 00:00:00 UTC 2018

Regulation of chitinase-3-like-1 in T cell elicits Th1 and cytotoxic responses to inhibit lung metastasis.

Nature communications

Kim DH,Park HJ,Lim S,Koo JH,Lee HG,Choi JO,Oh JH,Ha SJ,Kang MJ,Lee CM,Lee CG,Elias JA,Choi JM

Published figure using Granzyme B monoclonal antibody (Product # 25-8898-82) in Flow Cytometry

Mon Feb 05 00:00:00 UTC 2018

MAIT cells contribute to protection against lethal influenza infection in vivo.

Nature communications

van Wilgenburg B,Loh L,Chen Z,Pediongco TJ,Wang H,Shi M,Zhao Z,Koutsakos M,Nüssing S,Sant S,Wang Z,D'Souza C,Jia X,Almeida CF,Kostenko L,Eckle SBG,Meehan BS,Kallies A,Godfrey DI,Reading PC,Corbett AJ,McCluskey J,Klenerman P,Kedzierska K,Hinks TSC

Published figure using Granzyme B monoclonal antibody (Product # 25-8898-82) in Flow Cytometry

Fri Nov 09 00:00:00 UTC 2018

Crosstalks between mTORC1 and mTORC2 variagate cytokine signaling to control NK maturation and effector function.

Nature communications

Wang F,Meng M,Mo B,Yang Y,Ji Y,Huang P,Lai W,Pan X,You T,Luo H,Guan X,Deng Y,Yuan S,Chu J,Namaka M,Hughes T,Ye L,Yu J,Li X,Deng Y

Published figure using Granzyme B monoclonal antibody (Product # 25-8898-82) in Flow Cytometry

Mon Nov 19 00:00:00 UTC 2018

Inflammatory Ly6C^high Monocytes Protect against Candidiasis through IL-15-Driven NK Cell/Neutrophil Activation.

Immunity

Domínguez-Andrés J,Feo-Lucas L,Minguito de la Escalera M,González L,López-Bravo M,Ardavín C

25-8898 was used in Flow cytometry/Cell sorting to explore the role of inflammatory Ly6Chigh monocytes in natural killer cell-mediated neutrophil activation during the innate immune response against Candida albicans.

Tue Jun 20 00:00:00 UTC 2017

Combined antiangiogenic and anti-PD-L1 therapy stimulates tumor immunity through HEV formation.

Science translational medicine

Allen E,Jabouille A,Rivera LB,Lodewijckx I,Missiaen R,Steri V,Feyen K,Tawney J,Hanahan D,Michael IP,Bergers G

Published figure using Granzyme B monoclonal antibody (Product # 25-8898-82) in Flow Cytometry

Wed Apr 12 00:00:00 UTC 2017

Egr2 and 3 control adaptive immune responses by temporally uncoupling expansion from T cell differentiation.

The Journal of experimental medicine

Miao T,Symonds ALJ,Singh R,Symonds JD,Ogbe A,Omodho B,Zhu B,Li S,Wang P

Published figure using Granzyme B monoclonal antibody (Product # 25-8898-82) in Flow Cytometry

Mon Jun 05 00:00:00 UTC 2017

1810011o10 Rik Inhibits the Antitumor Effect of Intratumoral CD8⁺ T Cells through Suppression of Notch2 Pathway in a Murine Hepatocellular Carcinoma Model.

Frontiers in immunology

Dai K,Huang L,Huang YB,Chen ZB,Yang LH,Jiang YA

25-8898 was used in Flow cytometry/Cell sorting to disclose a novel mechanism underlying the negative regulation of antitumour CD8(+) T cells during HCC progression.

Wed Nov 20 00:00:00 UTC 2019

Targeted calcium influx boosts cytotoxic T lymphocyte function in the tumour microenvironment.

Nature communications

Kim KD,Bae S,Capece T,Nedelkovska H,de Rubio RG,Smrcka AV,Jun CD,Jung W,Park B,Kim TI,Kim M

Published figure using Granzyme B monoclonal antibody (Product # 25-8898-82) in Flow Cytometry

Mon May 15 00:00:00 UTC 2017

Loss of HIF-1α in natural killer cells inhibits tumour growth by stimulating non-productive angiogenesis.

Nature communications

Krzywinska E,Kantari-Mimoun C,Kerdiles Y,Sobecki M,Isagawa T,Gotthardt D,Castells M,Haubold J,Millien C,Viel T,Tavitian B,Takeda N,Fandrey J,Vivier E,Sexl V,Stockmann C

Published figure using Granzyme B monoclonal antibody (Product # 25-8898-82) in Flow Cytometry

Fri Nov 17 00:00:00 UTC 2017

Lack of the programmed death-1 receptor renders host susceptible to enteric microbial infection through impairing the production of the mucosal natural killer cell effector molecules.

Journal of leukocyte biology

Solaymani-Mohammadi S,Lakhdari O,Minev I,Shenouda S,Frey BF,Billeskov R,Singer SM,Berzofsky JA,Eckmann L,Kagnoff MF

25-8898 was used in Flow cytometry/Cell sorting to identify the role of the programmed death-1 receptor in early host microbial defence at the intestinal mucosa.

Tue Mar 01 00:00:00 UTC 2016

The stress kinase GCN2 does not mediate suppression of antitumor T cell responses by tryptophan catabolism in experimental melanomas.

Oncoimmunology

Sonner JK,Deumelandt K,Ott M,Thomé CM,Rauschenbach KJ,Schulz S,Munteanu B,Mohapatra S,Adam I,Hofer AC,Feuerer M,Opitz CA,Hopf C,Wick W,Platten M

Published figure using Granzyme B monoclonal antibody (Product # 25-8898-82) in Flow Cytometry

Tue Jan 12 00:00:00 UTC 2021

Caribbean and La Réunion Chikungunya Virus Isolates Differ in Their Capacity To Induce Proinflammatory Th1 and NK Cell Responses and Acute Joint Pathology.

Journal of virology

Teo TH,Her Z,Tan JJ,Lum FM,Lee WW,Chan YH,Ong RY,Kam YW,Leparc-Goffart I,Gallian P,Rénia L,de Lamballerie X,Ng LF

Published figure using Granzyme B monoclonal antibody (Product # 25-8898-82) in Flow Cytometry

Sat Aug 01 00:00:00 UTC 2015

Intestinal microbiota modulates gluten-induced immunopathology in humanized mice.

The American journal of pathology

Galipeau HJ,McCarville JL,Huebener S,Litwin O,Meisel M,Jabri B,Sanz Y,Murray JA,Jordana M,Alaedini A,Chirdo FG,Verdu EF

25-8898 was used in Flow cytometry/Cell sorting to assess whether specific microbiota compositions influence immune responses to gluten in mice expressing the human DQ8 gene, which confers moderate CD genetic susceptibility.

Sun Nov 01 00:00:00 UTC 2015

mTORC1-dependent metabolic reprogramming is a prerequisite for NK cell effector function.

Journal of immunology (Baltimore, Md. : 1950)

Donnelly RP,Loftus RM,Keating SE,Liou KT,Biron CA,Gardiner CM,Finlay DK

25-8898 was used in Flow cytometry/Cell sorting to investigate whether mTORC1-controlled metabolism underpins normal natural killer cell proinflammatory functions.

Sat Nov 01 00:00:00 UTC 2014

Damage to the blood-brain barrier during experimental cerebral malaria results from synergistic effects of CD8+ T cells with different specificities.

Infection and immunity

Poh CM,Howland SW,Grotenbreg GM,Rénia L

25-8898 was used in Immunocytochemistry to show that blood-brain barrier damage during experimental cerebral malaria is mediated by synergistic actions of CD8T cells recognizing different malaria-specific epitopes.

Sat Nov 01 00:00:00 UTC 2014

Tim-3 negatively mediates natural killer cell function in LPS-induced endotoxic shock.

PloS one

Hou H,Liu W,Wu S,Lu Y,Peng J,Zhu Y,Lu Y,Wang F,Sun Z

Published figure using Granzyme B monoclonal antibody (Product # 25-8898-82) in Flow Cytometry

Mon Jul 06 00:00:00 UTC 2015

Metastasis is regulated via microRNA-200/ZEB1 axis control of tumour cell PD-L1 expression and intratumoral immunosuppression.

Nature communications

Chen L,Gibbons DL,Goswami S,Cortez MA,Ahn YH,Byers LA,Zhang X,Yi X,Dwyer D,Lin W,Diao L,Wang J,Roybal J,Patel M,Ungewiss C,Peng D,Antonia S,Mediavilla-Varela M,Robertson G,Suraokar M,Welsh JW,Erez B,Wistuba II,Chen L,Peng D,Wang S,Ullrich SE,Heymach JV,Kurie JM,Qin FX

Published figure using Granzyme B monoclonal antibody (Product # 25-8898-82) in Flow Cytometry

Tue Oct 28 00:00:00 UTC 2014

Both NK cell-intrinsic and -extrinsic STAT1 signaling are required for NK cell response against vaccinia virus.

Journal of immunology (Baltimore, Md. : 1950)

Fortin C,Huang X,Yang Y

25-8898 was used in Flow cytometry/Cell sorting to explore the role of STAT1 in natural killer cell responses to vaccinia virus infection in vivo.

Mon Jul 01 00:00:00 UTC 2013

Bioinformatics

Protein Aliases: CCP1; CTLA-1; Cytotoxic cell protease 1; Cytotoxic T lymphocyte associated serine esterase 1; Fragmentin-2; Granzyme B(G,H); GranzymeB; Human lymphocyte protein (Hlp); OTTHUMP00000028189

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Gene Aliases: AI553453; CCP-1/C11; CCP1; Ctla-1; Ctla1; GZB; Gzmb

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UniProt ID: (Mouse) P04187

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Entrez Gene ID: (Mouse) 14939

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Function(s)

serine-type endopeptidase activity protein binding peptidase activity serine-type peptidase activity hydrolase activity

Process(es)

T cell mediated cytotoxicity proteolysis apoptotic process granzyme-mediated apoptotic signaling pathway protein processing cytolysis

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Granzyme B Monoclonal Antibody (NGZB), PE-Cyanine7, eBioscience™

Granzyme B Antibody (25-8898-82) in Flow

Product Details

25-8898-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

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RRID

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