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Granzyme H Antibody (PA5-47129) in ICC/IF
Immunocytochemistry analysis of Granzyme H in immersion fixed human peripheral blood mononuclear cells (PBMCs). Samples were incubated in Granzyme H polyclonal antibody (Product # PA5-47129) using a dilution of 5 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytop... View More
Product Details
PA5-47129
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Granzyme H Glu19-Leu246
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2609230
Product Specific Information
This antibody detects both the pro and active forms of human Granzyme H in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human Granzyme B is observed and approximately 5% cross-reactivity with recombinant mouse (rm) Granzyme C, rmGranzyme D, and rmGranzyme G is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.01 EU per 1 µg of the antibody by the LAL method.
Target Information
This gene encodes a member of the peptidase S1 family of serine proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate a chymotrypsin-like protease. This protein is reported to be constitutively expressed in the NK (natural killer) cells of the immune system and may play a role in the cytotoxic arm of the innate immune response by inducing target cell death and by directly cleaving substrates in pathogen-infected cells. This gene is present in a gene cluster with another member of the granzyme subfamily on chromosome 14.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Cathepsin G-like 2; cathepsin G-like 2, protein h-CCPX; CCP-X; CSP-C; CTSGL2; Cytotoxic serine protease C; Cytotoxic T-lymphocyte proteinase; cytotoxic T-lymphocyte-associated serine esterase 1; cytotoxin serine protease-C; Granzyme H; granzyme H (cathepsin G-like 2, protein h-CCPX)
Gene Aliases: CCP-X; CGL-2; CGL2; CSP-C; CTLA1; CTSGL2; GZMH
UniProt ID: (Human) P20718
Entrez Gene ID: (Human) 2999
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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