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Invitrogen
Green Renilla luciferase Polyclonal Antibody
FIGURE: 1 / 8
Green Renilla luciferase Antibody (PA1-180) in ICC/IF
Immunofluorescent analysis of Green Renilla luciferase (from the Sea pansy Renilla reniformis) in untransfected (UT, left panel) and pCMV-Green Renilla (GR) luciferase transfected (green, right panel) 293 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 2% Blocker BSA (Product # 37525) for 15 minutes at room temperature.... View More
Product Details
PA1-180
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Renilla
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Purified full length recombinant Green Renilla luciferase expressed in E. coli.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS with 30% glycerol, 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2539911
Product Specific Information
Western blot analysis of PA1-180 detects a prominent ~36 kDa protein in Green Renilla luciferase expressing cells. Additional unspecific bands are also detected in control cells with increased exposure time. This antibody has also been successfully tested in IP, Immunofluorescence, and neutralization assays.
Green Renilla luciferase activity can be measured using the Thermo Fisher Firefly Luciferase Flash or Glow Assay Kits or Luciferase Dual Assay Kits.
Target Information
The Green Renilla luciferase is a 36kDa protein produced by a derivative of the wild type Renilla luciferase gene from the sea pansy, Renilla reniformis. Compared to the wild type luciferase, Green Renilla is more stable in serum and has an the emission spectrum that is shifted toward the green region. The protein provides extremely bright flash signal that decays rapidly. The Renilla Flash and Glow Assay Kit reagents allow for Glow (stabilized signal with ~3 hours halflife) or Flash type bioluminescence experiments and are optimized for best results with Thermo Scientific Green Renilla Luc Plasmids. Bioluminescence provides researchers with a non-radioactive assay for measuring transcriptional activity of regulatory elements. Reporter activity can be used to determine promoter activity, to study upstream pathway activity in the cell, or to determine effect of activators or inhibitors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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